Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests

Kim, Hyo Eun; Schuck, Ariadna; Lee, See Hi; Lee, Yong Kyu; Kang, Minhee; Kim, Yong−Sang

doi:10.1016/j.bios.2021.113168

Cited by 90 publications

(52 citation statements)

References 52 publications

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“…More than 40% of the published results in 2020 used lateral flow based detection of RPA amplicons. Nevertheless CRISPR-Cas based systems 12 are used in the same way like colorimetric assays 13 , gel electrophoresis 10 , electrochemical detection systems 14 , 15 and microarray based techniques 16 .…”

Section: Introductionmentioning

confidence: 99%

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

Warmt

Fenzel

Henkel

et al. 2021

Sci Rep

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In this report we describe Cy5-dUTP labelling of recombinase-polymerase-amplification (RPA) products directly during the amplification process for the first time. Nucleic acid amplification techniques, especially polymerase-chain-reaction as well as various isothermal amplification methods such as RPA, becomes a promising tool in the detection of pathogens and target specific genes. Actually, RPA even provides more advantages. This isothermal method got popular in point of care diagnostics because of its speed and sensitivity but requires pre-labelled primer or probes for a following detection of the amplicons. To overcome this disadvantages, we performed an labelling of RPA-amplicons with Cy5-dUTP without the need of pre-labelled primers. The amplification results of various multiple antibiotic resistance genes indicating great potential as a flexible and promising tool with high specific and sensitive detection capabilities of the target genes. After the determination of an appropriate rate of 1% Cy5-dUTP and 99% unlabelled dTTP we were able to detect the blaCTX-M15 gene in less than 1.6E−03 ng genomic DNA corresponding to approximately 200 cfu of Escherichia coli cells in only 40 min amplification time.

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Section: Introductionmentioning

confidence: 99%

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

Warmt

Fenzel

Henkel

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, a relatively long analysis time of at least 3 h and the need for professional experts with sophisticated analysis procedures limit the rapid screening of samples in large quantities [207]. Hence, the development of a new type of biosensor with a POCT platform is imperative for the detection of viral species to prevent the spread of SARS-CoV-2 by isolating patients [208][209][210][211][212]. Step-like response transitions upon the increasing concentration of IgG with the limit of detection of 1 ng/mL in the inset.…”

Section: Biosensorsmentioning

confidence: 99%

Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea

Choi

Lee

Choi

et al. 2022

Sensors

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Herein, state-of-the-art research advances in South Korea regarding the development of chemical sensing materials and fully integrated Internet of Things (IoT) sensing platforms were comprehensively reviewed for verifying the applicability of such sensing systems in point-of-care testing (POCT). Various organic/inorganic nanomaterials were synthesized and characterized to understand their fundamental chemical sensing mechanisms upon exposure to target analytes. Moreover, the applicability of nanomaterials integrated with IoT-based signal transducers for the real-time and on-site analysis of chemical species was verified. In this review, we focused on the development of noble nanostructures and signal transduction techniques for use in IoT sensing platforms, and based on their applications, such systems were classified into gas sensors, ion sensors, and biosensors. A future perspective for the development of chemical sensors was discussed for application to next-generation POCT systems that facilitate rapid and multiplexed screening of various analytes.

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“…Other research attempts supported the role of various technological arrangements as POCT. For instance, the combination of electrochemical biosensors and the recombinase polymerase amplification (RPA) assay is a rapid, sensitive and convenient platform that can be potentially used as a Point-of-Care test to diagnose COVID-19 [208]. The combination worked without expensive thermo-cycling equipment and allowed the detection of multiple genes by differential pulse voltammetry, which was possible because of the hybridisation of the RPA amplicon with modified primers followed by amplicons' build-up.…”

Section: Point Of Care Testing (Poct)mentioning

confidence: 99%

Point-of-Care Testing—The Key in the Battle against SARS-CoV-2 Pandemic

et al. 2021

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The deleterious effects of the coronavirus disease 2019 (COVID-19) pandemic urged the development of diagnostic tools to manage the spread of disease. Currently, the “gold standard” involves the use of quantitative real-time polymerase chain reaction (qRT-PCR) for SARS-CoV-2 detection. Even though it is sensitive, specific and applicable for large batches of samples, qRT-PCR is labour-intensive, time-consuming, requires trained personnel and is not available in remote settings. This review summarizes and compares the available strategies for COVID-19: serological testing, Point-of-Care Testing, nanotechnology-based approaches and biosensors. Last but not least, we address the advantages and limitations of these methods as well as perspectives in COVID-19 diagnostics. The effort is constantly focused on understanding the quickly changing landscape of available diagnostic testing of COVID-19 at the clinical levels and introducing reliable and rapid screening point of care testing. The last approach is key to aid the clinical decision-making process for infection control, enhancing an appropriate treatment strategy and prompt isolation of asymptomatic/mild cases. As a viable alternative, Point-of-Care Testing (POCT) is typically low-cost and user-friendly, hence harbouring tremendous potential for rapid COVID-19 diagnosis.

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Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests

Cited by 90 publications

References 52 publications

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes

Nanomaterials for IoT Sensing Platforms and Point-of-Care Applications in South Korea

Point-of-Care Testing—The Key in the Battle against SARS-CoV-2 Pandemic

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