Sensitive electrochemical detection of A549 exosomes based on DNA/ferrocene-modified single-walled carbon nanotube complex

Si, Fuchun; Liu, Zenghui; Li, Jinge; Yang, Huaixia; Liu, Yanju; Kong, Jinming

doi:10.1016/j.ab.2022.114971

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…44–46 Some biosensors exhibit better analytical performance in certain aspects than ours, but their wide application faces the following challenges: (1) the electrochemical techniques need to be improved for clinical sample analysis because the electrode generally suffers from biofouling and inactivation, thus influencing their stability and sensitivity. 47 (2) Some DNA sensors require tedious nucleic acid sequence designing and covalent assembly, leading to increased complexity, testing duration and cost. 48,49 (3) As for colorimetric detection, a high amount of H 2 O 2 might induce a harsh reaction environment and destroy the sensing element.…”

Section: Resultsmentioning

confidence: 99%

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

Zheng,

Huang,

Liu

et al. 2024

Anal. Methods

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Section: Resultsmentioning

confidence: 99%

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

Zheng,

Huang,

Liu

et al. 2024

Anal. Methods

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“…CNTs can also be used to develop electrochemical-based EV sensors. For example, Si et al reported electrochemcial detection of lung cancer exosomes using DNA/ferrocene-modified SWCNT [ 176 ]. Sahraei et al developed a biodegradable electrochemical paper-based device (ePAD) by incorporating synthetic carbon, a silver ink screen, and AuNPs on paper [ 177 ].…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

et al. 2023

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Extracellular vesicles (EVs) have emerged as a novel resource of biomarkers for cancer and certain other diseases. Probing EVs in body fluids has become of major interest in the past decade in the development of a new-generation liquid biopsy for cancer diagnosis and monitoring. However, sensitive and specific molecular detection and analysis are challenging, due to the small size of EVs, low amount of antigens on individual EVs, and the complex biofluid matrix. Nanomaterials have been widely used in the technological development of protein and nucleic acid-based EV detection and analysis, owing to the unique structure and functional properties of materials at the nanometer scale. In this review, we summarize various nanomaterial-based analytical technologies for molecular EV detection and analysis. We discuss these technologies based on the major types of nanomaterials, including plasmonic, fluorescent, magnetic, organic, carbon-based, and certain other nanostructures. For each type of nanomaterial, functional properties are briefly described, followed by the applications of the nanomaterials for EV biomarker detection, profiling, and analysis in terms of detection mechanisms.

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“…Labelling of the surface-bound analyte was carried out not by the typical molecular recognition of aptamers, but by the formation of PO 4 3− -Ti 4+ -PO 4 3− bridges between DNA backbones and the exosome (see Figure 22 ). Such an immunoassay enables analyte detection by SWV in the range 4.66 × 10 6 –9.32 × 10 9 exosomes/mL with an LOD of 9.38 × 10 4 exosomes/mL [ 131 ].…”

Section: Role Of Carbon Nanomaterials and Their Composites In The Des...mentioning

confidence: 99%

“… A549 exosomes detection principle by means of sandwich immunoassay labeled with SWCNTs conjugated with DNA and ferrocene. Adapted with permission from Si et al [ 131 ] (copyright © (2023), Elsevier). …”

Section: Figurementioning

confidence: 99%

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

Szymczyk

Ziółkowski

Malinowska

2023

Sensors

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To meet the requirements of novel therapies, effective treatments should be supported by diagnostic tools characterized by appropriate analytical and working parameters. These are, in particular, fast and reliable responses that are proportional to analyte concentration, with low detection limits, high selectivity, cost-efficient construction, and portability, allowing for the development of point-of-care devices. Biosensors using nucleic acids as receptors has turned out to be an effective approach for meeting the abovementioned requirements. Careful design of the receptor layers will allow them to obtain DNA biosensors that are dedicated to almost any analyte, including ions, low and high molecular weight compounds, nucleic acids, proteins, and even whole cells. The impulse for the application of carbon nanomaterials in electrochemical DNA biosensors is rooted in the possibility to further influence their analytical parameters and adjust them to the chosen analysis. Such nanomaterials enable the lowering of the detection limit, the extension of the biosensor linear response, or the increase in selectivity. This is possible thanks to their high conductivity, large surface-to-area ratio, ease of chemical modification, and introduction of other nanomaterials, such as nanoparticles, into the carbon structures. This review discusses the recent advances on the design and application of carbon nanomaterials in electrochemical DNA biosensors that are dedicated especially to modern medical diagnostics.

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Sensitive electrochemical detection of A549 exosomes based on DNA/ferrocene-modified single-walled carbon nanotube complex

Cited by 7 publications

References 36 publications

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

Nanomaterials for Molecular Detection and Analysis of Extracellular Vesicles

Modern Electrochemical Biosensing Based on Nucleic Acids and Carbon Nanomaterials

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