Sensitive electrochemical detection of hydroxyl radical based on MBs–DNA–AgNPs nanocomposite

Yang, Yan; Zhou, Jun; Zhang, Yunsong; Zou, Qiong; Zhang, Xiaohua; Chen, Jinhua

doi:10.1016/j.snb.2013.03.071

Cited by 28 publications

(10 citation statements)

References 36 publications

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“…salicylic acid, phenylalanine) with high kinetic constants. To date, quantitative determination of •OH has been performed using different analytical techniques such as fluorescence, chemiluminescence, electron spin resonance, colorimetric, and electrochemical methods . However, electrochemical techniques have been proven to be highly efficient tool for the determination of •OH as they ensure their direct quantification with high sensitivity, selectivity and ease of operation.…”

Section: Introductionmentioning

confidence: 99%

Real Time Detection of Hazardous Hydroxyl Radical Using an Electrochemical Approach

et al. 2019

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The proposed work demonstrates an electrochemical strategy for hydroxyl radical (•OH) detection based on aptamer immobilized glassy carbon electrode (GCE). To enhance the sensitivity of the detection method, nitrogen doped porous carbon nanostructures (N−C) and gold nanoparticles (AuNPs) were used as signal amplifiers. During biosensor fabrication, thiolated DNA and 6‐mercaptohexanol (MCH) were self assembled on N−C/AuNPs modified GCE surface. The fabricated biosensor was characterized electrochemically by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) which justified its high electronic conductivity. The aptamer immobilized electrode (N−C/AuNPs/DNA/MCH/GCE) was immersed in Fenton's reagent where oxidative damage of DNA occurred due to generated •OH. The DNA damage was monitored by hexaammineruthenium(III) (Ru(NH3)63+) which acted as electrochemical probe for sensitive detection of •OH. Square wave voltammetry (SWV) was used for •OH biosensing using simple, sensitive and rapid strategy at the fabricated electrode.

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Section: Introductionmentioning

confidence: 99%

Real Time Detection of Hazardous Hydroxyl Radical Using an Electrochemical Approach

et al. 2019

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“…The developed method showed a linear range from 4.06

10 −15 to 1.23

10 −13 M, and the detection limit is 1.35

10 −15 M (S/N = 3). As shown in Table 1 , compared with other methods, the prepared sensor in this paper showed a lower linear range and detection limit [ 42 , 44 , 45 , 46 , 47 ]. The results proved that the carboxylated- g -C 3 N 4 NP S had a great effect on the electrochemical response of the electrode reaction for • OH and provided a suitable microenvironment for ssDNA and MB to transfer electrons with underlying GCE.…”

Section: Resultsmentioning

confidence: 89%

A Water-Dispersible Carboxylated Carbon Nitride Nanoparticles-Based Electrochemical Platform for Direct Reporting of Hydroxyl Radical in Meat

Han

Huang

Sun

et al. 2021

Foods

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In this paper, carboxylated carbon nitride nanoparticles (carboxylated-g-C3N4 NPs) were prepared through a one-step molten salts method. The synthesized material was characterized by transmission electron microscope (TEM), Fourier transform-infrared spectra (FTIR), and X-ray photoelectron spectroscopy (XPS), etc. An electrochemical sensor based on single-stranded oligonucleotide/carboxylated-g-C3N4/chitosan/glassy carbon electrode (ssDNA/carboxylated-g-C3N4/chitosan/GCE) was constructed for determination of the hydroxyl radical (•OH), and methylene blue (MB) was used as a signal molecule. The sensor showed a suitable electrochemical response toward •OH from 4.06 to 122.79 fM with a detection limit of 1.35 fM. The selectivity, reproducibility, and stability were also presented. Application of the sensor to real meat samples (i.e., pork, chicken, shrimp, and sausage) was performed, and the results indicated the proposed method could be used to detect •OH in practical samples. The proposed sensor holds a great promise to be applied in the fields of food safety.

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“…However, excessive cOH is extremely harmful to human health and leads to a series of physiological diseases like aging and Parkinson's disease by reacting with numerous biomacromolecules including DNA bases, lipids, and proteins. 6 Therefore, rapid, sensitive and accurate detection of intracellular cOH concentration is of vital importance in order to well understand its biological impact and timely hamper its damage. Whereas, selectively monitoring cOH is very difficult due to its short life-time (about 10 À9 s).…”

Section: Introductionmentioning

confidence: 99%

A sensitive BODIPY-based fluorescent probe for detecting endogenous hydroxyl radicals in living cells

Bian

Chen

et al. 2020

RSC Adv.

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Sensitive electrochemical detection of hydroxyl radical based on MBs–DNA–AgNPs nanocomposite

Cited by 28 publications

References 36 publications

Real Time Detection of Hazardous Hydroxyl Radical Using an Electrochemical Approach

Real Time Detection of Hazardous Hydroxyl Radical Using an Electrochemical Approach

A Water-Dispersible Carboxylated Carbon Nitride Nanoparticles-Based Electrochemical Platform for Direct Reporting of Hydroxyl Radical in Meat

A sensitive BODIPY-based fluorescent probe for detecting endogenous hydroxyl radicals in living cells

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