Sensitive electrochemical determination of yohimbine in primary bark of natural aphrodisiacs using boron-doped diamond electrode

Abstract

For the first time, a simple and sensitive analytical method for the direct determination of yohimbine is presented using differential pulse voltammetry with a boron-doped diamond electrode. Two irreversible oxidation peaks, a distinct one at +0.80 and a second poorly-defined one at +1.65 V, were observed when cyclic voltammetry was carried out in Britton-Robinson buffer solution at pH 7 (vs. Ag/AgCl). With optimized differential pulse voltammetric parameters (pulse amplitude 100 mV, pulse time 25 ms, step potential 5 mV and scan rate 10 mV s À1), the current response of yohimbine at +0.80 V was linearly proportional to the concentration in the range from 0.25 to 90.9 mmol L À1 with a low detection limit of 0.13 mmol L À1 (0.046 mg L À1) and a good repeatability (relative standard deviation of 2.5% at 18.4 mmol L À1 for n ¼ 6). The practical applicability of the developed method was demonstrated by the assessment of the total content of yohimbine in extracts of the primary bark of natural aphrodisiacs such as Pausinystalia yohimbe and Rauvolfia serpentina with recoveries in the range of 92-97%. The proposed electrochemical procedure represents an inexpensive and effective analytical alternative for the quality control analysis of products containing yohimbine and other biologically and structurally related alkaloids used as natural dietary supplements.

Flow-injection amperometric determination of yohimbine alkaloid in dietary supplements using a boron-doped diamond electrode

“…The analytical performance of herein described method is comparable with that recently achieved on BDD electrode in batch arrangement with the linear concentration range from 0.25 to 90.9 M and the detection limit of 0.13 M [18]. However, the significance of proposed FIA methodology embodies in rapid determination of YOH with sampling frequency about 70 injections h −1 .…”

“…Some data regarding the electrochemical behavior of YOH on BDD electrode have been reported by Swain and co-workers [17]. Our research group has recently published the paper regarding the voltammetric determination of YOH by differential pulse voltammetry using BDD electrode [18]. In this study, two irreversible oxidation peaks, a distinct (quantification) peak at +0.80 V assigned to the oxidation of the hydroxyl group of YOH including the loss of two electrons and two protons to give a ring ketone and a second poorly defined peak at +1.65 V associated with the oxidative deprotonization of the carbon at position 6, were observed in Britton-Robinson buffer solution at pH 7.…”

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

“…Nowadays, the use of boron-doped diamond electrode (BDD) is significantly increasing and it is one of the most preferred solid electrode material due to a chemical inertness, very low background currents, low adsorption, and a wide applicable potential window (Svorc et al 2014;Pecková et al 2009). The electrode is also environmentally friendly, highly sensitive, long-term stable and it can be used for the determination of different biologically active compounds with significant importance in the field of food, environment, and drug analysis (Stanković and Kalcher 2015;Stanković 2015).…”

Voltammetric determination of harmaline in natural food products using boron-doped diamond electrode

“…BDD electrodes represent effective alternatives to conventional carbonaceous electrodes, and our research group extensively used them as versatile electrochemical tool for the sensitive determination of miscellaneous biologically active compounds [28][29][30][31]. Recently, several applications of BDD electrode for electrochemical determination of harmful organic compounds in food and beverage samples such as kresoxim-methyl [32] and dimethoform [33] as well as important additives in food industry such as caffeine [31], yohimbine [34], vanillin [35], chlorogenic acid [36] and capsaicin [37] were reported.…”