Sensitive enantiomer-specific high-performance liquid chromatographic analysis of methamphetamine and amphetamine from serum using precolumn fluorescent derivatization

Hutchaleelaha, Athiwat; Walters, Andrea E.; Chow, H.‐H. Sherry; Mayersohn, Michael

doi:10.1016/0378-4347(94)00229-0

Cited by 25 publications

(9 citation statements)

References 20 publications

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“…The original SPE procedure developed for the pretreatment of the samples (using C2 cartridges) allowed to obtain good purification of the biological samples and satisfactory precision in relatively shorter times using low volumes of solvents. The extraction yields (>97%) obtained with the proposed method are higher than those obtained by other authors who used liquid -liquid extraction [18,25,27,36,37], or SPE with C18 [16,28], Oasis HLB or MCX cartridges [14,38]. The method is very selective: none of the drugs tested caused any interference in the analysis.…”

Section: Discussioncontrasting

confidence: 52%

“…The sensitivity is higher when using precolumn derivatisation with UV detection [17]. Precolumn derivatisation with fluorimetric detection has also been carried out using labelling reagents such as 9-fluorenylethylchloroformate (overnight reaction) [18], 4-(N,N-dimethylaminosulphonyl)-7-fluoro-1,2,3-benzoxadiazole [19], 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride [20,21] and FITC [22] obtaining good results but making the method quite complicated and time-consuming. Gas-chromatographic methods coupled with MS [23 -25] or LC-MS methods are reported, but these techniques are more expensive and complicated [26,27].…”

Section: Introductionmentioning

confidence: 99%

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Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Bugamelli

Mandrioli

Cavallini

et al. 2006

J of Separation Science

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A precise and feasible HPLC method has been developed for the analysis of amphetamine (AMPH), methamphetamine (MAMPH) and methylenedioxymethamphetamine (MDMA, ecstasy) in human urine. A chromatographic run on a C8 Genesis (150 mm x 4.6 mm, 5 microm) column maintained at 30 degrees C lasts about 17 min, using a mobile phase composed of ACN (12%) and a pH 2.5 phosphate buffer (88%) containing 0.3% triethylamine. Mirtazapine was used as the internal standard. Good linearity was found in the 100-2000 ng/mL concentration range for AMPH and MAMPH and in the 12-2000 ng/mL concentration range for MDMA. The pretreatment of urine samples was carried out by means of a careful SPE procedure on C2 cartridges. The extraction yields were very satisfactory for all analytes, with average values greater than 97%. The leading conditions allowed the determination of AMPH, MAMPH and MDMA with satisfactory precision and accuracy. The method has been successfully applied to the determination of the analytes in urine of AMPH users.

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Section: Discussioncontrasting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Bugamelli

Mandrioli

Cavallini

et al. 2006

J of Separation Science

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Section: Methodsmentioning

confidence: 99%

“…11,12 Serum samples were analyzed for the enantiomers of MAP and AP. 11 Urine samples were assayed for enantiomers of MAP, AP, and both unconjugated and conjugated forms of the enantiomers of OHMAP and OHAP. 12 Linear standard curves for the assay of enantiomers of MAP and AP in serum were in the concentration range of 5-250 ng/mL with the intraday and interday coefficients of variation in the range of 2.1-18.6%.…”

Section: Methodsmentioning

confidence: 99%

Influence of Activated Charcoal on the Disposition Kinetics of Methamphetamine Enantiomers in the Rat Following Intravenous Dosing

Hutchaleelaha

Mayersohn

1996

Journal of Pharmaceutical Sciences

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Methamphetamine (MAP) is a central nervous system stimulant that is widely abused by populations of several countries. There is no specific antidote for the treatment of an overdose. Activated charcoal administered orally has been used to enhance the systemic elimination of certain toxic substances via "gastrointestinal dialysis". The results of in vitro studies have shown that MAP can be rapidly adsorbed from solution by activated charcoal. We have evaluated the effect of a single oral dose of activated charcoal on the disposition kinetics of MAP following a short iv infusion. Male Sprague-Dawley rats were given an oral dose of activated charcoal (Actidose-aqua, 1 g/kg) 10 min before a short iv infusion of racemic MAP; whereas the control group was given an equivalent volume of water. Enantiomers of MAP and metabolites in serum and urine were analyzed by an enantiomer-specific method which employed HPLC and detection of a fluorescent derivative. There were no differences in any of the disposition parameters between the two groups. Within each group, the clearance (CLs) of l-MAP was greater than that of d-MAP. However, there were no differences in the steady-state volume of distribution (Vss). The CLs (mL/(min kg)) and Vss (L/kg) values for l- and d-MAP in the control group were (mean +/- SD): 55.8 +/- 20.4, 48.7 +/- 17.9, 2.64 +/- 1.16, and 2.90 +/- 1.36, respectively. The corresponding values in the charcoal-pretreated group were (mean +/- SD): 57.4 +/- 23.4, 51.1 +/- 20.7, 2.79 +/- 1.32, and 2.98 +/- 1.47. These results suggest that oral activated charcoal does not enhance the elimination of MAP from the body.

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“…Usually, enantiomer ratio of methamphetamine is measured through capillary electrophoresis (CE) [6][7][8], HPLC [9][10][11][12] and GC-MS [13][14][15][16][17][18][19][20]. Among the analytical techniques, GC-MS has been widely used for its convenience.…”

Section: Introductionmentioning

confidence: 99%

Monitoring precursor chemicals of methamphetamine through enantiomer profiling

Lee

Yang

Han

et al. 2007

Forensic Science International

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Sensitive enantiomer-specific high-performance liquid chromatographic analysis of methamphetamine and amphetamine from serum using precolumn fluorescent derivatization

Cited by 25 publications

References 20 publications

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Determination of amphetamines in human urine by liquid chromatography with fluorimetric detection using a solid‐phase extraction procedure

Influence of Activated Charcoal on the Disposition Kinetics of Methamphetamine Enantiomers in the Rat Following Intravenous Dosing

Monitoring precursor chemicals of methamphetamine through enantiomer profiling

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