Sensitive fluorescence monitoring of carbohydrates eluted by a borate mobile phase from an anion-exchange column

Indirect cationic chromatography with fluorometric detection

“…The fluorescence of aqueous solutions of cerium(III) has been known for some time and its use in analytical chemistry has been reported (11)(12)(13)(14)(15). Prevously in our laboratory, cerium(III) was characterized as an eluent for indirect photometric chromatography of cations (16).…”

Instrumental effects on limits of detection in gas phase fluorescence detection of gas chromatographic effluents

“…Two new procedures are presented for determining the precision of rate measurements under noise limited conditions and the contribution of sample Introduction Imprecision to the total precision. Fluorometric kinetics-based analysis is concerned with measuring the rate of a reaction by monitoring the fluorescence 1 Present address, Department of Chemistry, University of Wisconsin-Eau Claire, Eau Claire, Wis. 54701 signal from a species involved in the reaction.…”

“…Molecular fluorescence, as well as being a powerful analytical tool by itself, has become well-established as an ancillary technique to both liquid (1)(2)(3)(4)(5)(6)(7)(8)(9)(10) and thin layer (11)(12)(13)(14)(15) chromatography. There have also been several examples of molecular fluorescence being used to identify and quantify gas chromatographic eluents (16)(17)(18)(19)(20)(21).…”

High Performance Liquid Chromatography of Natural Products

“…Ion-exchange chromatography continues to be widely used in carbohydrate separations, particularly for mono-, di-, and tri-saccharides, with some workers using anion exchange (216)(217)(218), some using cation exchange (217)(218)(219)(220), and others using combinations of both methods (221)(222)(223)(224)(225)(226). The use of borate complexes to separate sugars on ion-exchange columns has also been reported (227)(228)(229), while the incorporation of boronic acid residues into the column has also yielded good separations of vicinal diols (230). Molecular exclusion chromatography has been applied to the separation of polysaccharides ( 231), oligosaccharides (232) and substituted carbohydrates (233).…”