Sensitive fluorescent sensor for the fuzzy exosomes in serum based on the exosome imprinted polymer sandwiched with aggregation induced emission

Liao, Zerong; Peng, Jirun; Shi, Chen; Zhang, Pengjie; Chen, Hui; Feng, Dongwei; Zhang, Tuanjie; Ye, Kaida; Deng, Yulin; Dong, Yuping; Li, Geng

doi:10.1016/j.snb.2021.131182

Cited by 17 publications

(11 citation statements)

References 82 publications

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“…Compared with the methods for EV determination reported previously, the GIP sensor has the lowest LOD (Table S1). In addition, compared with the molecularly imprinted polymer-based EV sensor, − the GIP sensor has the advantages of easy realization of template molecular elution and EV recognition.…”

Section: Resultsmentioning

confidence: 99%

“…Molecularly imprinted films, such as custom polymeric films with complementary binding sites to the template molecule, have a strong affinity and selectivity for the template molecule compared to the natural receptor. Recently, analytical methods have been developed for detecting EVs using a whole imprinted method using EVs directly as templates. − However, EVs, as vesicles with a particle size of 30–150 nm, do not have a rigid structure and are easily deformed, making it difficult to form regular cavities in the molecularly imprinted polymer during its preparation, thus affecting the selective readsorption of EVs in the sample. In addition, it is also difficult to perform operations, such as elution and readsorption, due to its large size and fragility.…”

mentioning

confidence: 99%

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An Electrochemiluminescent Sensor Based on Glycosyl Imprinting and Aptamer for the Detection of Cancer-Related Extracellular Vesicles

Jiang,

Luo,

Yang

et al. 2024

Anal. Chem.

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Cancer-related extracellular vesicles (EVs) are considered important biomarkers for cancer diagnosis because they can convey a large amount of information about tumor cells. In order to detect cancer-related EVs efficiently, an electrochemiluminescence (ECL) sensor for the specific identification and highly sensitive detection of EVs in the plasma of cancer patients was constructed based on dual recognitions by glycosyl-imprinted polymer (GIP) and aptamer. The characteristic glycosyl Neu5Ac-α-(2,6)-Gal-β-(1−4)-GlcNAc trisaccharide on the surface of EVs was used as a template molecule and 3-aminophenylboronic acid as a functional monomer to form a glycosyl-imprinted polymer by electropolymerization. After glycosyl elution, the imprinted film specifically recognized and adsorbed the EVs in the sample, and then the CD63 aptamer− bipyridine ruthenium (Aptamer−Ru(bpy)) was added to combine with the CD63 glycoprotein on the extracellular vesicle's surface, thus providing secondary recognition of the EVs. Finally, the EVs were quantitatively detected according to the ECL signal produced by the labeled bipyridine ruthenium. When more EVs were captured by the imprinted film, more probes were obtained after incubation, and the ECL signal was stronger. Under the optimized conditions, the ECL signal showed a good linear relationship with the concentration of EVs in the range of 9.5 × 10 2 to 9.5 × 10 7 particles/mL, and the limit of detection was 641 particles/mL. The GIP sensor can discriminate between the EV contents of cancer patients and healthy controls with high accuracy. Because of its affordability, high sensitivity, and ease of use, it is anticipated to be employed for cancer early detection and diagnosis.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

An Electrochemiluminescent Sensor Based on Glycosyl Imprinting and Aptamer for the Detection of Cancer-Related Extracellular Vesicles

Jiang,

Luo,

Yang

et al. 2024

Anal. Chem.

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“…In vivo imaging of cancer biomarkers is another possible alternative to tissue biopsy [ 93 ]. Either way, MIP-based sensors and imaging probes have demonstrated great results for the detection of cancer biomarkers, such as nucleic acids, proteins, exosomes, and cancer cells [ 94 – 96 ]. As each biomarker comes with its own detection challenges, MIP-based technologies, as well as offering great stability and sensitivity, present an unprecedented adaptability of the detection probes [ 96 – 98 ].…”

Section: Mip-based Cancer Diagnosismentioning

confidence: 99%

“…The GO quenched the fluorescence of the aptamer-bound FAM; however, in the presence of the target, the oligonucleotide would be released from the GO and the fluorescence would increase proportionally to the availability of the analyte. Liao et al developed the ‘turn-on’ fluorescent sensor to detect the presence of exosomes in patient serum with a sensitivity comparable to the enzyme-linked immunosorbent assay [ 94 ]. The detection process was achieved through aptamer-mediated aggregation-induced emission.…”

Section: Mip-based Cancer Diagnosismentioning

confidence: 99%

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

et al. 2023

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Cancer is a disease caused by abnormal cell growth that spreads through other parts of the body and threatens life by destroying healthy tissues. Therefore, numerous techniques have been employed not only to diagnose and monitor the progress of cancer in a precise manner but also to develop appropriate therapeutic agents with enhanced efficacy and safety profiles. In this regard, molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. Taken together, the topics discussed in this review provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment. Graphical Abstract Molecularly imprinted polymers (MIPs), synthetic receptors that recognize targeted molecules with high affinity and selectivity, have been intensively investigated as one of the most attractive biomaterials for cancer theragnostic approaches. This review describes diverse synthesis strategies to provide the rationale behind these synthetic antibodies and provides a selective overview of the recent progress in the in vitro and in vivo targeting of cancer biomarkers for diagnosis and therapeutic applications. The topics discussed in this review aim to provide concise guidelines for the development of novel MIP-based systems to diagnose cancer more precisely and promote successful treatment.

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“…Furthermore, exosomes are easy to identify, attracting attention as optimal biomarkers in liquid cancer biopsies. Recently, efforts have been made to detect exosomes based on various detection platforms, such as on electricity- [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ], electrochemical- [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 ], and optical-based platforms [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis

et al. 2022

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Exosomes have been gaining attention for early cancer diagnosis owing to their biological functions in cells. Several studies have reported the relevance of exosomes in various diseases, including pancreatic cancer, retroperitoneal fibrosis, obesity, neurodegenerative diseases, and atherosclerosis. Particularly, exosomes are regarded as biomarkers for cancer diagnosis and can be detected in biofluids, such as saliva, urine, peritoneal fluid, and blood. Thus, exosomes are advantageous for cancer liquid biopsies as they overcome the current limitations of cancer tissue biopsies. Several studies have reported methods for exosome isolation, and analysis for cancer diagnosis. However, further clinical trials are still required to determine accurate exosome concentration quantification methods. Recently, various biosensors have been developed to detect exosomal biomarkers, including tumor-derived exosomes, nucleic acids, and proteins. Among these, the exact quantification of tumor-derived exosomes is a serious obstacle to the clinical use of liquid biopsies. Precise detection of exosome concentration is difficult because it requires clinical sample pretreatment. To solve this problem, the use of the nanobiohybrid material-based biosensor provides improved sensitivity and selectivity. The present review will discuss recent progress in exosome biosensors consisting of nanomaterials and biomaterial hybrids for electrochemical, electrical, and optical-based biosensors.

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Sensitive fluorescent sensor for the fuzzy exosomes in serum based on the exosome imprinted polymer sandwiched with aggregation induced emission

Cited by 17 publications

References 82 publications

An Electrochemiluminescent Sensor Based on Glycosyl Imprinting and Aptamer for the Detection of Cancer-Related Extracellular Vesicles

An Electrochemiluminescent Sensor Based on Glycosyl Imprinting and Aptamer for the Detection of Cancer-Related Extracellular Vesicles

Molecularly imprinted polymers (MIPs): emerging biomaterials for cancer theragnostic applications

Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis

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