Sensitive fluorometric method for tissue tocopherol analysis

Taylor, Steve L.; Lamden, Merton P.; Tappel, Al L.

doi:10.1007/bf02532898

Cited by 342 publications

(96 citation statements)

References 30 publications

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“…Circulating carotenoids (␣-and ␤-carotene, cryptoxanthin, lutein/zeaxanthin, and lycopene), in addition to retinol, and ␣-and ␥-tocopherol, were measured on samples from free-ranging rhinoceroses using reversed-phase high performance liquid chromatography (Sowell et al, 1994), and 25(OH)D 3 was measured by vitamin D-binding protein assay (Chen et al, 1990). Other tissues were saponified prior to extraction (Taylor et al, 1976), and concentrations of vitamins A and E were measured via modifications of the general method of Taylor et al (1976;see Barker et al, 1998 for details).…”

Section: Methodsmentioning

confidence: 99%

Fat Soluble Vitamins in Blood and Tissues of Free-Ranging and Captive Rhinoceros

Clauß

Jessup

Norkus

et al. 2002

Journal of Wildlife Diseases

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ABSTRACT. Several disease syndromes in captive rhinoceroses have been linked to low vitamin status. Blood samples from captive and free-ranging black (Diceros bicornis) and white rhinoceros (Ceratotherium simum) and tissue samples of captive individuals from four rhinoceros species were analysed for vitamins A and E. Circulating vitamin A levels measured as retinol for freeranging versus captive black and white rhinoceros were 0.04 (Ϯ0.03 SD) vs. 0.08 (Ϯ0.08) and 0.07 (Ϯ0.04) vs. 0.06 (Ϯ0.02) g/ml, respectively. Circulating vitamin E levels measured as ␣-tocopherol were 0.58 (Ϯ0.30) vs. 0.84 (Ϯ0.96) and 0.62 (Ϯ0.48) vs. 0.77 (Ϯ0.32) g/ml, respectively. In contrast to earlier findings, there was no significant difference in vitamin E concentration between captive and free-ranging black rhinoceros. When the samples of captive black rhinoceros were grouped into those taken before 1990 and after 1990, however, those collected before 1990 had significantly lower (P Ͻ 0.001) vitamin E levels (0.46 Ϯ 0.83 g/ml) and those collected in 1990 or later significantly higher (P Ͻ 0.001) vitamin E levels (1.03 Ϯ 1.04 g/ml) than the captive population as a whole. This is probably due to increased dietary supplementation. There were significant differences in circulating vitamin concentrations in black rhinoceroses from different regions in the wild. Serum 25-hydroxy (OH) vitamin D 3 averaged 55.7 ng/ml in freeranging rhinoceroses; no carotenoids were detected in any blood samples. Captive black and white rhinoceroses appear to be adequately supplemented in vitamin A and E. Captive Indian rhinoceroses (Rhinoceros unicornis) had significantly lower vitamin A concentrations in blood (P Ͻ 0.001) and higher vitamin A concentrations in liver tissue samples (P Ͻ 0.001) than other rhinoceros species. Equine requirements are not recommended as a model for rhinoceros vitamin requirements.

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Section: Methodsmentioning

confidence: 99%

Fat Soluble Vitamins in Blood and Tissues of Free-Ranging and Captive Rhinoceros

Clauß

Jessup

Norkus

et al. 2002

Journal of Wildlife Diseases

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“…Both tissue and serum samples were read at 532 nm and were reported as nmol/g tissue or nmol/mL, respectively. The tissue and serum α-tocopherol concentrations were determined by fluorometric methods (Hitachi F-2000 fluorometer) with the samples read at excitation λ 290 nm and emission λ 329 nm (19,20), and they were expressed in µg/g tissue or µg/mL, respectively. Serum, total cholesterol, and triglycerides concentrations were measured enzymatically (Flexor E, Vital Scientific, the Netherlands) and reported as mmol/L.…”

Section: Biochemical Estimationsmentioning

confidence: 99%

Lipid peroxidation in neoplastic tissue of dogs with mammary cancer fed with different kinds of diet

KARAYANNOPOULOU¹,

FYTIANOU²,

ASSALOUMIDIS³

et al. 2013

Turk J Vet Anim Sci

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“…Vitamin E concentrations in RBC was determined by ā uorimetric technique, sensitive in the range of 0.10±50 mg tocopherol/ml, with a recovery of between 91±100% (Taylor et al, 1976). Lipid peroxidation was estimated indirectly as a derivative of malondialdehyde (MDA), generated by the reactivity of MDA with thiobarbituric acid (TBA).…”

Section: Methodsmentioning

confidence: 99%

Erythrocyte membrane fatty acid composition of smokers and non-smokers: effects of vitamin E supplementation

Brown¹,

Morrice²,

Duthie³

1998

Eur J Clin Nutr

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Objective: Adequate dietary intakes of vitamin E and essential polyunsaturated fatty acids are important to maintain cell membrane integrity, and de®ciencies have been associated with smoking related cardiovascular disease. Suf®cient vitamin E is required to prevent free radical mediated peroxidation of membrane lipids. Consequently, smokers may have a greater requirement for this antioxidant. To investigate, we assessed the concurrent in¯uences of smoking, vitamin E supplementation and red blood cell (RBC) PUFA composition on the susceptibility of the cells to lipid peroxidation in adult males. Design and subjects: Thirty male smokers and thirty male non-smokers were randomly ascribed to daily 280 mg vitamin E or placebo supplements for 10 weeks. RBC were analysed at weeks 0 and 10 for fatty acid methyl esters, vitamin E, and their susceptibility to in vitro H 2 O 2 induced lipid peroxidation. Results: Concentrations of essential fatty acids (EFA) in RBC were lower in smokers than in non-smokers. Supplementation with vitamin E increased levels of RBC EFA in smokers to match those of non-smokers. Furthermore, the ratio of vitamin E to PUFA in RBC from smokers and non-smokers was inversely correlated with their susceptibility to peroxidation. Conclusions: An adequate vitamin E to PUFA ratio is required to protect cell membranes from oxidative damage. The signi®cant correlation between susceptibility to peroxidation and the PUFA content of RBC before supplementation suggests an inadequate intake of vitamin E in relation to PUFA intake. Moreover, the requirement for vitamin E appears to be greater in smokers than in non-smokers.

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Sensitive fluorometric method for tissue tocopherol analysis

Cited by 342 publications

References 30 publications

Fat Soluble Vitamins in Blood and Tissues of Free-Ranging and Captive Rhinoceros

Fat Soluble Vitamins in Blood and Tissues of Free-Ranging and Captive Rhinoceros

Lipid peroxidation in neoplastic tissue of dogs with mammary cancer fed with different kinds of diet

Erythrocyte membrane fatty acid composition of smokers and non-smokers: effects of vitamin E supplementation

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