Sensitive HPLC method for quantitation of paclitaxel (Genexol®) in biological samples with application to preclinical pharmacokinetics and biodistribution

Kim, Sung Chul; Yu, Jae-Won; Lee, Jang-Won; Park, Eun-Seok; Chi, Sang-Cheol

doi:10.1016/j.jpba.2005.02.023

Cited by 60 publications

(35 citation statements)

References 22 publications

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“…The current study details a high performance liquid chromatography (HPLC) method to quantify PTX concentrations in mouse plasma and tissues following intravenous administration of PTX/TS-CS-PEG-FA micelles in tumor-bearing mice. The separation and analysis of PTX in biological fluids and tissues has been mainly performed by HPLC techniques using different clean-up procedures including protein precipitation (PP) (17)(18)(19)(20), solidphase extraction (SPE) (21,22), and multiple step of liquid-liquid extraction (LLE) (23)(24)(25)(26)(27)(28). Although direct injection of samples after precipitation of the plasma proteins does not require an evaporation step and make the procedure rapid and simple, this method could deteriorate the chromatographic column due to inadequate protein precipitation.…”

Section: Introductionmentioning

confidence: 99%

“…In most instances, LLE has been considered as the main procedure for the isolation and determination of drug substances in biological matrices. Several publications have appeared with regard to HPLC determination of PTX in biological fluids after LLE (23)(24)(25)(26)(27)(28). Some assays employ a relatively large volume (0.5-2 ml) of biological sample to maximize the sensitivity of the assays (24,25).…”

Section: Introductionmentioning

confidence: 99%

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A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

et al. 2015

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-A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of paclitaxel (PTX) in plasma, various organs and tumor tissues of tumor-bearing mice. Tissue specimens of liver, kidneys, spleen, lungs, heart and tumor were separately homogenized in normal saline. Plasma or tissue homogenate (250 µl) containing PTX and internal standard (diazepam) were extracted by diethyl ether (6 ml). The separation was achieved on a µ-Bondapak C 18 HPLC column using sodium acetate buffer solution (0.01 M)/acetonitrile (58/42 v/v) at pH 5 ± 0.1 and flow rate of 1.9 mL/min. The effluent was monitored at 227 nm and column temperature was adjusted at 58 º C. The internal standard and PTX were eluted at 4.2 and 5.2 min, respectively and no interfering peaks were observed. Calibration curves were linear over the concentration range of 0.25-10 µg/ml of PTX in plasma and 0.3-20 µg/ml PTX in tissue homogenates with acceptable precision and accuracy (<15%). The mean recoveries of the drug after plasma extraction was 87.4% ± 3.6 while those of tissue homogenates ranged from 62.1± 4.5 to 75.5± 3.2 depending on the type of tissues studied. PTX was stable in samples with no evidence of degradation during 3 freeze-thaw cycles and 3 months storage at −70 °C. The developed HPLC method was applied to quantify PTX in the mouse plasma and tissues after intravenous administration of 10 mg equivalent PTX/Kg dose of PTX-loaded tocopherol succinate-chitosan-polyethylene glycol-folate (TS-CS-PEG-FA) micelles formulation or Anzatax ® (Cremophor ®

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

et al. 2015

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“…Monitoring of paclitaxel concentration and verapamil in rats blood plasma is possible using a HPLC method with isocratic mode and UV detection [67]. First, the plasma samples were exposed to one-step liquid-liquid extraction by terc-buthyl methyl ether.…”

Section: Methods For Monitoring Of Paclitaxel Concentrationmentioning

confidence: 99%

Paclitaxel as an anticancer agent: isolation, activity, synthesis and stability

et al. 2011

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AbstractPaclitaxel is isolated from the Pacific yew. It can be obtained from the European yew, but only after chemical modification of the isolated compound by a semi-synthesis procedure. The procedure for total synthesis of paclitaxel is very complicated, involving multiple steps, and the yields of paclitaxel are meagre. This substance is also a metabolite of certain kinds of fungus. The microbiological pathway for producing paclitaxel compared with isolation from plant material involves shorter procuction times but a small yield. Cyclodextrins are usually used for improving the solubility of paclitaxel in aqueous media, with polymeric and other substances added. Paclitaxel has anticancer activity and use for preparing the formulations intravenously administrated to patients with tumors. The paclitaxel concentration in these formulations is determined using validated HPLC methods.

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“…This expansion of use is largely due to the efficacy of paclitaxel in treatment of a wide range of solid tumors [28]. This drug moves to block mitotic activity through interference with the formation of microtubules [29], thus preventing cancer cell division. However, due to the prevalence of significant side effects, paclitaxel is almost always used in a comparatively low dose to other bioactive agents [30].…”

Section: Metronidazolementioning

confidence: 99%

“…This need for a precise window of treatment combined with the important nature of the drugs indications make paclitaxel an important drug for quantification. HPLC is uniquely suited for the low levels of drug used as well as the common use of paclitaxel with other drugs and excipients such as Chremophor EL ® [28][29][30][31]. The hydrophobic nature of the drug does necessitate different mobile phase ratios than other drugs described, but hydrophobic columns can be used and detection is usually around 230 nm [28][29][30][31].…”

Section: Metronidazolementioning

confidence: 99%

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

Corbett¹

2013

Mod Chem Appl

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Capability to quantify drug release is vital in many areas of pharmaceutical research. Accurate, rapid and repeatable detection of pharmaceutical agents allows for comparisons between drug delivery constructs prior to in vivo studies as well as identification of therapeutic levels in samples both in vitro and in vivo. Perhaps the foremost scientific method for monitoring of therapeutics is High Performance Liquid Chromatography. This process allows for clear and simple quantification of therapeutic presence in a sample, with an inherent or induced chromophore, through the detection of bound specimen to the utilized column. Multiple drugs can be used through this process and exemplary methods are presented for the pharmaceutics; cefuroxime, clindamycin, dexamethasone, dicloxacillin, doxycycline, metronidazole, oxymetazoline, paclitaxel, tobramycin, and vancomycin. Each of these drugs is analyzed using Reversed-Phase High Performance Liquid Chromatography utilizing a hydrophobic column. Independent, repeatable methods are developed for each drug. Where necessary a pre-column derivatization is used to allow for visualization of otherwise undetectable tobramycin. In the case of each drug, a standard curve is presented to show the linearity of response for the proposed method within a range of absorbance. This work shows the ability to analyze multiple drugs with a single simple, quick, cost effective system and detection methods are evaluated for each drug with a R 2 value of greater than 0.99.

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Sensitive HPLC method for quantitation of paclitaxel (Genexol®) in biological samples with application to preclinical pharmacokinetics and biodistribution

Cited by 60 publications

References 22 publications

A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

A Rapid and Sensitive HPLC Method for Quantitation of Paclitaxel in Biological Samples using Liquid-Liquid Extraction and UV Detection: Application to Pharmacokinetics and Tissues Distribution Study of Paclitaxel Loaded Targeted Polymeric Micelles in.....

Paclitaxel as an anticancer agent: isolation, activity, synthesis and stability

Development and Utilization of Reversed Phase High Performance Liquid Chromatography Methods for a Series of Therapeutic Agents

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