Sensitive method for the determination of the antiarrhythmic drug detajmium in serum by solid-phase extraction and high-performance liquid chromatography with fluorimetric detection

Liquid chromatographic assay with fluorescence detection to determine ajmaline in serum from patients with suspected Brugada syndrome

“…Further, the presence of typical electrocardiogram (ECG) abnormalities can be investigated with or without a drug challenge test [1]. The most efficient drug in unmasking the Brugada 1,9 .0 2,7 .0 10,15 .0 12,17 ]nonadeca-2(7),3,5-triene-14,18-diol). This sodium channel blocking, class 1A anti-arrhythmic drug that was shown to be safe when used in a challenge test for the diagnosis of the syndrome [1,4].…”

“…The first assay that was useful for pharmacokinetic evaluation of the drug was reported by Clemans et al [6] using GC-MS after an extremely tedious sample pre-treatment using five liquid-liquid extraction steps and derivatization. Easier assays all use liquid chromatography with fluorescent detection after an ethereal liquid-liquid extraction [7,8], automated solid-phase extraction [9] or on-line extraction [10] procedure. In addition, protein precipitation with acetonitrile was investigated shortly as an alternative for liquid-liquid extraction by Koppel et al [8].…”