Sensitive methods for detection of SARS-CoV-2 RNA

Chen, Xi

doi:10.1016/bs.mim.2021.06.001

Cited by 5 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the regression equations were different for each of the Allplex™ assays. Differences are most likely due to primer- and probe design, inhibitors or RNA detection methods [11] .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performances of four commercially available assays for the identification of SARS-CoV-2, influenza type A/B virus and RSV

Houwen¹,

Lisdonk²,

Bolier³

et al. 2023

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

“…However, the regression equations were different for each of the Allplex™ assays. Differences are most likely due to primer- and probe design, inhibitors or RNA detection methods [11] .…”

Section: Discussionmentioning

confidence: 99%

Diagnostic performances of four commercially available assays for the identification of SARS-CoV-2, influenza type A/B virus and RSV

Houwen¹,

Lisdonk²,

Bolier³

et al. 2023

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

“…In addition, a study revealed that the polymerase amplification (RT-RPA) method has been introduced as an ultrahigh-sensitive method in disease diagnosis (24).…”

Section: Specimen Type and Collection Methodsmentioning

confidence: 99%

Molecular Diagnosis of COVID-19; Biosafety and Pre-analytical Recommendations

Vasei,

Jafari,

Falah Azad

et al. 2023

Iran J Pathol

View full text Add to dashboard Cite

From the beginning of the COVID-19 epidemic, clinical laboratories around the world have been involved with tests for detection of SARS-CoV-2. At present, RT-PCR (real-time reverse transcription polymerase chain reaction assay) is seen as the gold standard for identifying the virus. Many factors are involved in achieving the highest accuracy in this test, including parameters related to the pre-analysis stage. Having instructions on the type of sample, how to take the sample, and its storage and transportation help control the interfering factors at this stage. Studies have shown that pre-analytical factors might be the cause of the high SARS-CoV-2 test false-negative rates. Also, the safety of personnel in molecular laboratories is of utmost importance, and it requires strict guidelines to ensure the safety of exposed individuals and prevent the virus from spreading. Since the onset of the outbreak, various instructions and guidelines have been developed in this field by the institutions and the Ministry of Health of each country; these guidelines are seriously in need of integration and operation. In this study, we try to collect all the information and research done from the beginning of this pandemic in December 2019 - August 2022 concerning biosafety and protective measures, sample types, sampling methods, container, and storage solutions, sampling equipment, and sample storage and transportation for molecular testing of SARS-CoV-2.

show abstract

“…WGS can be used for virus detection as well, but it costs too much both in economy and time. Nowadays reverse transcription-polymerase chain amplification (RT-PCR) has been widely used for the detection of viral RNA [23]. Real-time fluorescent quantitative PCR is regarded as the gold standard for coronavirus detection owing to its high sensitivity, strong specificity, low cost, rapidity and simplicity [24].…”

Section: Diagnose Covid-19mentioning

confidence: 99%

The role of genomics in COVID-19

Ji¹

2022

HSET

View full text Add to dashboard Cite

Since the coronavirus disease 2019 (COVID-19) pandemic swept the globe, the infectious population has proliferated dramatically with each passing day. With the spread of the pandemic around the world, the virus mutated to different lineages in different regions. To better control COVID-19 and trace the source of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-COV-2), genomics has played an indispensable role in the epidemic. Scientists have carried out relevant research in genomics and made significant discoveries recently. The author focuses on analyzing the application of genomics to the novel coronavirus pneumonia and delves into the significance of genomics to COVID-19. This paper demonstrates the significance of genomics in tracing the origin of viruses, forecasting the trajectory of the pandemic, and diagnosing and treating COVID-19. This has been of great help in beginning to bring the pandemic under control.

show abstract

Sensitive methods for detection of SARS-CoV-2 RNA

Cited by 5 publications

References 46 publications

Diagnostic performances of four commercially available assays for the identification of SARS-CoV-2, influenza type A/B virus and RSV

Diagnostic performances of four commercially available assays for the identification of SARS-CoV-2, influenza type A/B virus and RSV

Molecular Diagnosis of COVID-19; Biosafety and Pre-analytical Recommendations

The role of genomics in COVID-19

Contact Info

Product

Resources

About