Sensitive monitoring of RNA transcription by optical amplification of cationic conjugated polymers

Poly(1,3,5-tris(4-ethynylphenyl)-benzene) Conjugated Polymers as Electrochemical Sensors for Hydrogen Peroxide Detection

“…The TEM image of PTEPB (Figure S1a) shows the presence of overlapping nanosheets, as was previously reported. 26 The local chemical structures of PTEPB are investigated by 13 C cross-polarization magic angle spinning (CP-MAS) solid-state NMR spectroscopy. As shown in Figure S1b, the peaks at approximately 92.2 ppm can be assigned to the 1,3-diyne carbons in PTEPB.…”

“…Conjugated polymers (CPs) are a unique class of materials consisting of π-conjugated frameworks that have drawn increasing attention because of the remarkable features of CPs, such as having tunable structures and optoelectronic properties. − Currently, numerous CPs with different structures and special properties have been developed by employing various chemical reactions, building blocks, and synthetic methods. , The distinctive advantages of CPs have made these polymers an ideal type of biomedical material in many fields, including cell imaging, , fluorescence sensors, drug delivery, , and disease diagnosis, , due to the excellent photostability, low cytotoxicity, and good biocompatibility of CPs. − …”

“…2−5 Currently, numerous CPs with different structures and special properties have been developed by employing various chemical reactions, building blocks, and synthetic methods. 6,7 The distinctive advantages of CPs have made these polymers an ideal type of biomedical material in many fields, including cell imaging, 8,9 fluorescence sensors, 10 drug delivery, 11,12 and disease diagnosis, 13,14 due to the excellent photostability, low cytotoxicity, and good biocompatibility of CPs. 15−18 In recent years, there have been many excellent studies focused on the optical and electrochemical properties of CPs, especially those with special structures.…”

The First FRET-Based RNA Aptamer NanoKit for Sensitively and Specifically Detecting c-di-GMP

“…Recently, biosensors based on conjugated polymers (CPs) have attracted great research interest in many biological and chemical applications, due to their energy-harvesting nature and Förster resonance energy transfer (FRET) property, in which a proper acceptor can efficiently transfer energy and lead to significantly quenched donor fluorescence intensity, thereby amplifying the acceptor fluorescence to enhance the signals. , Particularly, cationic CPs (CCPs) are applied in the optical sensing of DNA/RNA through the electrostatic complexation, which combines the unique optical amplification properties of π-conjugated polymers and the electrostatic behaviors of polyelectrolytes, making them an ideal FRET donor for oligonucleotides detection. − Importantly, the multiple absorbing units and backbone of CCPs can amplify the fluorescence intensity of a tightly bound acceptor fluorophore by approximately 1 order of magnitude, allowing for trace detection of target substances. − Therefore, combining CCPs and RNA aptamers may provide a new platform for sensitive and specific c-di-GMP detection.…”

Incompatibility of DFHBI based fluorescent RNA aptamers with particular commercial cell-free expression systems

“…1 They consist of single stranded RNA sequences that have been selectively evolved and designed to bind and activate a fluorophore in their solution 2 , which can then be detected by fluorescence spectrophotometry or fluorescence microscopy. 3 These sequences can be used as a direct fluorescent reporter of transcription without the need for translation 4 , or can be included as a tag on other RNA transcripts and visualised in vivo to locate an RNA molecule within a live cell. 5,6 Commonly used examples of fluorophore-binding RNAs include Broccoli 7 and Spinach, 8 which use 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) as a fluorophore.…”