Sensitive Nonradiometric Method for Determining Thymidine Kinase 1 Activity

Abstract

Conclusion:The TK1 enzyme-labeled immunoassay uses a stable substrate, is precise, appears to be accurate, and

Liquid chromatography–tandem mass spectrometry method for quantification of thymidine kinase activity in human serum by monitoring the conversion of 3′-deoxy-3′-fluorothymidine to 3′-deoxy-3′-fluorothymidine monophosphate

“…The reaction is performed at 37 • C in a 0.1 M sodium acetate phosphorylation buffer supplemented with enzyme co-factors and stabilizing reagents [11,23]. ATP serves as a phosphate donor in the reaction.…”

“…In particular, TK1 can phosphorylate nucleotide analogs with modifications at the 5 -position of the pyrimidine ring, and the 3 -position of the ribose including 5-fluoro-2 -dideoxythymidine and 3 -azido-3 -deoxythymidine (Zidovudine) [9,10]. These nucleotide analogs can therefore be used to monitor the activity of TK1 [3,6,11]. For example, 18 F-3 -deoxy-3 -fluorothymidine ( 18 F-FLT), has been used to monitor tumor proliferation via positron emission tomography [12][13][14].…”

“…This assay is time-consuming and requires radioactive waste management. A competitive enzyme-linked immunosorbent assay (ELISA) has also been used for measuring serum TK1 activity for measuring phosphorylation of the selective TK1 substrate 3 -azido-2 -deoxythymidine (AZT) [11]. Alternatively, 5-bromodeoxyuridine (BrdU) incorporation into DNA can be monitored by ELISA [16].…”

The Thymidine Kinase-1(tk-1) as a Potential Tumor Marker: Serum Levels in Serum of Patients With Solid and System Malignance Neoplasms

“…Проводилось сравнение тест-систем для выявления ТК-1, изучалась корреляция между сыворо-точной концентрацией и активностью ТК-1 при различных ЗНО и в норме [15][16][17][18].…”

Parsimonious Selection of Useful Genes in Microarray Gene Expression Data

“…[NM 003258] TK1-Thymidine kinase 1, soluble is a cytoplasmic enzyme. Studies have shown that in patients with primary breast cancer, high TK values were shown to be an important risk factor in nodenegative patients and seemed to be associated with beneficial effect of adjuvant chemotherapy [28]. [NM 003239] TGFB3-transforming growth factor, beta 3 is a potent growth inhibitor of normal epithelial cells.…”