Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Grabias, Bryan; Essuman, Edward; Quakyi, Isabella A.; Kumar, Sanjai

doi:10.1186/s12936-019-2743-9

Cited by 15 publications

(14 citation statements)

References 34 publications

(37 reference statements)

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“…Blood film microscopy and rapid diagnostic tests are the mainstay of malaria diagnosis that can adequately detect Plasmodium infections in patients with high levels of parasitaemia [3,4]. However, both methods lack the sensitivity to detect the infection in individuals carrying low parasite density [5,6]. Given that low-grade parasitaemia in asymptomatic individuals can persist for a year or more, important sources of further transmission must be considered.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of malaria parasite amongst patients in a displaced people’s camp in Sudan

Eshag¹,

Elnzer²,

Nahied³

et al. 2020

Trop Med Health

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Background: Despite the importance of epidemiological studies in the development of effective control strategies and provision of basic health services for refugees and internally displaced persons (IDPs), data on the prevalence of malaria are limited. Thus, this study was conducted to estimate the molecular prevalence of malaria amongst the displaced population in Ardamata IDP camp in Al-Geneina City, Sudan. Methods: A cross-sectional study was conducted from July 2018 to December 2018 to estimate malaria prevalence amongst the displaced population in Ardamata IDP camp in Al-Geneina City, Sudan. A total of 380 patients with suspected malaria were recruited. Nested polymerase chain reaction (nPCR) assays were performed to detect the Plasmodium genus and species. Results: Of 380 patients, 232 (61.1%) were positive for malaria. Plasmodium falciparum was the only prevalent species detected amongst the study population. nPCR analysis revealed that none of the samples had Plasmodium vivax, Plasmodium ovale or Plasmodium malariae. The malaria prevalence rate was higher amongst males (67.1%) than in females (56.8%), and gender was the only risk factor that was significantly associated with malaria infection (p = .042). Conclusions: Despite control programmes, malaria remains a significant cause of illness amongst a displaced population. The high prevalence of malaria infection in this study indicates that additional health facilities and control strategies should be implemented in displaced camps and the surrounding areas.

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Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of malaria parasite amongst patients in a displaced people’s camp in Sudan

Eshag¹,

Elnzer²,

Nahied³

et al. 2020

Trop Med Health

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show abstract

“…For example, for Plasmodium falciparum , a study determined by PCR the limit of detection at 6.5 parasites/µL in fecal samples from NHPs from the Brazilian Amazon [ 9 ]. In human blood samples, the limit of detection of Plasmodium falciparum ranges from 0.03 parasites/µL to 9 parasites/ml using methods such as qPCR [ 352 ] and RT-PCR [ 353 ]. The sensitivity of parasite DNA extraction for both stool and blood samples will depend on sample storage [ 354 ], DNA extraction methods [ 355 ] and parasite densities in the population and in individuals [ 356 , 357 ].…”

Section: Discussionmentioning

confidence: 99%

Zoonotic Blood-Borne Pathogens in Non-Human Primates in the Neotropical Region: A Systematic Review

2021

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A systematic review was carried out using PRISMA guidelines from 1927 until 2019 about blood-borne pathogens present in NHPs of the Neotropical region (i.e., South America and Middle America). Results: A total of 127 publications were found in several databases. We found in 25 genera (132 species) of NHPs a total of 56 blood-borne pathogens in 197 records where Protozoa has the highest number of records in neotropical NHPs (n = 128) compared to bacteria (n = 12) and viruses (n = 57). Plasmodium brasilianum and Trypanosoma cruzi are the most recorded protozoa in NHP. The neotropical primate genus with the highest number of blood-borne pathogens recorded is Alouatta sp. (n = 32). The use of non-invasive samples for neotropical NHPs remains poor in a group where several species are endangered or threatened. A combination of serological and molecular techniques is common when detecting blood-borne pathogens. Socioecological and ecological risk factors facilitate the transmission of these parasites. Finally, a large number of countries remain unsurveyed, such as Ecuador, which can be of public health importance. Conclusions and significance: NHPs are potential reservoirs of a large number of blood-borne pathogens. In Ecuador, research activities should be focused on bacteria and viruses, where there is a gap of information for neotropical NHPs, in order to implement surveillance programs with regular and effective monitoring protocols adapted to NHPs.

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“…The studies mentioned above were performed by detecting the Rep marker by conventional PCR. In the last decade, the detection of parasite DNA in the blood has been enhanced with the development of different real-time PCR systems [18][19][20]. Real-time PCR has several advantages over conventional PCR and includes speed, simplicity, reproducibility and quantitative capacity [21].…”

Section: Design Of Primersmentioning

confidence: 99%

Untitled

2019

Int J Trop Dis

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Early serological diagnosis of trichinellosis is affected by a long-term immunological "silent" period following infection. This emphasises the need for the development of sensitive diagnostic methods to be used when antibodies cannot be detected. In this study, we assessed the usefulness of three DNA sequences as a direct diagnosis method to detect early infection with Trichinella spiralis in peripheral blood by SYBR green real-time PCR using a murine model. Primers were designed from a nuclear repetitive DNA element (Rep), the nuclear ribosomal internal transcribed spacer 2 (ITS2) region, and the mitochondrial large subunit of the ribosomal RNA gene (LSU). CF-1 mice were orally inoculated with 500 muscle larvae of T. spiralis and molecular detection was assessed in blood samples between 1 and 20 days post-inoculation (pi). In addition, antibody detection was evaluated every 5 days by ELISA using excretory-secretory antigens. Results showed that the Rep primers were found to amplify between 5 and 19 days pi and the ITS2 and LSU primers between 6 and 15 days pi, whereas the antibodies were detected at 30 days pi. Therefore, this molecular system could be a useful tool for the detection of early T. spiralis infection, in particularly by using the repetitive DNA element.

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Sensitive real-time PCR detection of Plasmodium falciparum parasites in whole blood by erythrocyte membrane protein 1 gene amplification

Cited by 15 publications

References 34 publications

Molecular epidemiology of malaria parasite amongst patients in a displaced people’s camp in Sudan

Molecular epidemiology of malaria parasite amongst patients in a displaced people’s camp in Sudan

Zoonotic Blood-Borne Pathogens in Non-Human Primates in the Neotropical Region: A Systematic Review

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