Sensitive recognition of prostate‐specific antigen using biotinylated antibody encapsulated on D‐penicillamine decorated wrinkled silicate nanoparticles (WSN): An innovative sandwich‐type biosensor toward diagnosis of prostate cancer

Abstract

In this study, a new sandwich-type biosensor was developed to specific recognition of prostate-specific antigen (PSA) and early-stage diagnosis of prostate cancer using encapsulation of biotinylated antibody (Ab1) of prostate-specific antigen on D-penicillamine decorated wrinkled silicate nanoparticles (WSN). For the first time, KCC-1-NH-DPA was synthesized and used to immobilization of biomacromolecules. So, fabricated immunosensor was performed by on sandwich-type strategy. After fabrication of immunosensor, cyclic voltammetry, differential pulse voltammetry, and square wave voltammetry techniques were used to electrochemical evaluation of immune-platform for PSA detection. The proposed biocompatibility immune-platform provided a novel interface toward sensitive bioanalysis of PSA biomarker in human plasma samples. Due to the use of gold nanoparticles functionalized Cysteamine (Cys A) in the structure of the secondary antibody (Ab2 [HRP-Ab2]), the intensity of the electrochemical signal has increased, resulting in a more accurate detection of the target molecules. Under the right conditions, the engineering immunosensor provides good bioanalytical performance for determining the PSA biomarker in the linear range of 0.002 to 60 μg L À1 which low limit of quantification was 0.002 μg L À1 . As a result, it is suggested to use these immune-devices in the clinical pre-diagnosis of prostate cancer.

An innovative transportable immune device for the recognition of α-synuclein using KCC-1-nPr-CS2 modified silver nano-ink: integration of pen-on-paper technology with biosensing toward early-stage diagnosis of Parkinson's disease

“…36 Notably, the high surface-to-volume ratio of these advanced mesoporous nanomaterials can be a determinant factor in the sensitivity of the sensor. 37 So far, some of the modified KCC-1 including KCC-1-NH 2 -FA for sensing HT 29 cancer cells, 38 KCC-1-NH 2 -DPA for sensing prostate-specific antigen, 39 and KCC-1-NH 2 -Tb for detection of aflatoxin M1 40 have been applied successfully.…”

Accelerated synthesis of 1,8-dioxo-octahydroxanthene and 1,8-dioxo-decahydroacridine derivatives using dendritic mesoporous nanosilica functionalized by hexamethylenetetramine: a novel nanocatalyst

“…Also, the ability to loading various guest molecules, reactants, and solvents into their internal surface and create the available active catalytic centers, are the main advantages of these nanomaterials in different research areas. [40][41][42] Hexamine, 43 also known as methamine and hexamethylenetetramine (HMTA) (Scheme 1), is a white and biocompatible crystalline heterocyclic organic compound that is highly soluble in water and polar organic solvents. This ammable solid has a cage-like structure and it has been used in the synthesis of some chemical compounds like plastic, pharmaceuticals and rubber additives.…”