Sensitive time-resolved fluoroimmunoassay for quantitative determination of clothianidin in agricultural samples

Abstract

Europium (Eu(3+))-labeled antibody was used as a fluorescent label to develop a highly sensitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues in agricultural samples. Toward this goal, the Eu(3+)-labeled polyclonal antibody and goat anti-rabbit antibody were prepared for developing and evaluating direct competitive TRFIA (dc-TRFIA) and indirect competitive TRFIA (ic-TRFIA). Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 9.20 and 0.0909 μg/L for the dc-TRFIA and 2.07 and 0.0220 μg/L for the ic-TRFIA, respectively. The ic-TRFIA has no obvious cross-reactivity with the analogues of clothianidin except for dinotefuran. The average recoveries of clothianidin from spiked water, soil, cabbage, and rice samples were estimated to range from 74.1 to 115.9 %, with relative standard deviations of 3.3 to 11.7 %. The results of TRFIA for the blind samples were largely consistent with gas chromatography (R (2) = 0.9902). The optimized ic-TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural samples.

Sensitive time-resolved fluoroimmunoassay for the quantitative detection of okadaic acid

“…In this study, we established an indirect competition TRFIA for OA in shellfish. Goat anti-mouse IgG, i.e., secondary antibody, was labeled with europium for low detection limit and high sensitivity (Esteve-Turrillas et al, 2010;Sesay et al, 2013;Li et al, 2014;Sheng et al, 2020). Compared with labeling the primary antibody, labeling the secondary antibody avoids the interference of the marker to the binding site of the antigen on the first antibody.…”

Trends of Diagnostic Methods for Human Viral Diseases

“…The fluorophore generates fluorescence at a distinct wavelength when excited by light, which can be quantified using a timelapse fluorometer. The target antibody or antigen is marked using the time-resolved fluorescence immunoassay (TR-FIA) technique, which makes use of lanthanide elements with distinctive fluorescence properties and the associated chelates as markers [18,19]. It frequently makes use of antibody that is adhered to the microplate well's bottom.…”

Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments

“…B 0 /IC 50 was selected as important criteria to evaluate the performance of the immunoassays (Brun et al, 2005;Korpimaki et al, 2004). In addition, IC 50 and B 0 also were regarded as primary physicochemical factors to estimate the performance of the methods (Li et al, 2014).…”