Sensitivity and Specificity Improvement of an Ion Sensitive Field Effect Transistors-Based Biosensor for Potato Glycoalkaloids Detection

Abstract

Butyryl cholinesterase of different origin along with variations of the time of enzyme immobilization on the potentiometric transducer surface is offered to control the ion sensitive field effect transistor (ISFET)-based biosensor sensitivity. Because butyryl cholinesterase has been already used to develop the sensors for heavy metals, organophosphorus/carbamate pesticides, and steroidal glycoalkaloids analysis, the present study has been focused on the investigation and adjustment of the ISFET-based biosensor specificity exclusively to the glycoalkaloids. Utilization of ethylendiaminetetracetate (a complexon of heavy metal ions) and phosphotriesterase (a highly efficient catalyst for the hydrolysis of organophosphorus compounds) enabled the highly specific determination of glycoalkaloids at the background of lead and mercury (up to 500 microM of ions concentration) and paraoxon (up to 100 microM of pesticide concentration). The developed biosensor has been validated for glycoalkaloids detection in potato varieties cultivated in Ukraine, and the results obtained are compared to those measured by the methods of HPLC and TLC.

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

“…The selectivity was investigated by Korpan et al by adding the ethylenediamine tetraacetate in the working solution in order to decrease the interferences of heavy metals and also to co-immobilise phosphotriesterase to render the biosensor insensitive to organophosphorus insecticides [215].…”

Development of acetylcholinesterase biosensor based on CdTe quantum dots modified cysteamine self-assembled monolayers

“…The extensive use of organophosphorus and carbamate pesticides in modern agriculture has raised serious public concern regarding the environment and food safety [15][16][17][18]. In order to determine trace levels of these toxic compounds rapidly and reliably, various inhibition and non-inhibition biosensor systems based on the immobilization of acetylcholinesterase (AChE) or organophosphorus hydrolase (OPH) have been proposed [19,20].…”

Recent advances in biosensors based on enzyme inhibition

“…Another interesting approach was reported by Korpan et al for the detection of glycoalkaloids using acetylcholinesterase biosensor in presence of heavy metals and pesticides. To this regard, the interference of heavy metals was removed by complexing them with ethylendiamminetetraacetate, while the interference of organophosphate pesticides was reduced using the phosphotriesterase enzyme, able to hydrolyse these pesticides (Korpan et al, 2006).…”