Sensitivity and Specificity of Various Serological Tests for Detection of <i>Brucella spp</i>. Infection in Male Goats and Sheep

Abstract

Brucellosis is a zoonosis found throughout the world with major implications both in the field of public health and farming economy. It is most often caused by Brucella abortus and Brucella melitensis. This study aimed for evaluation of the sensitivity and specificity of immunochromatographic assay (ICA) and comparing the results with those obtained from Rose Bengal Plate Test (RBPT) and in direct Enzyme Linked Immunsorbent Assay (ELISA) for serodiagnosis of sheep and goat brucellosis. Out of 41 sera samples (21 ram and 20 male goats) which were examined, antibodies against Brucella were detected in ram 8 (38.09%), 6 (28.57%) and 16 (76.19%) in ram samples, whereas they were detected in 3 (15%), 1 (5%) and 12 (60%) serum sample of goats males by RBPT, (ICA) and iELISA respectively. The kappa value between iELISA and ICA was −0.08 and that between RBPT and ELISA was −0.01 in ram and the kappa value between iELISA and ICA was −0.06 and that between RBPT and ELISA was −0.14 in male goats. The sensitivity for RBPT and ICA were 37.5% and 25% while specificity was 60% and 60% in ram; whereas, in male goats, sensitivity for RBPT was 8.33% and ICA was 8.33% while specificity was 75% and 100% respectively. It can be concluded that the developed ICA is immunodiagnostic assay, and it is rapid, non-expensive, economical and suitable for large-scale screening in developing countries and rural areas. KeywordsBrucellosis, RBPT, ICA, iELISA, Sheep, Goat W. A. Ahmed et al. 99

Patho-Morphological Valuation of Acute Infection of Brucella melitensis in Goats

“…Serological tests are useful for screening large size herds, very easy to perform, convenient and cheap. Nevertheless, cross-reactions with non-specific antibodies may generate false positive results (Ahmed et al, 2016). Molecular diagnostic methods have considerably reduced this type of risk and are the most reliable tools in terms of sensitivity and specificity (Leyla et al, 2003).…”

Seroprevalence of Neospora caninum, Toxoplasma gondii and Brucella Species in Sheep

“…Numerous surveillances have reported a higher prevalence in males compared to females (Ahmed et al, 2016;Alrodhan, 2017 andAbdelbaset et al, 2018). The increased incidence of infection in males could be attributed to their regular movement, such as during grazing, trading activities, or mating.…”