Sensitivity comparison of absorption and grating-based phase tomography of paraffin-embedded human brain tissue

Bikis, Christos; Rodgers, Griffin; Deyhle, Hans; Thalmann, Peter; Hipp, Alexander; Beckmann, Felix; Weitkamp, Timm; Theocharis, Stamatios; Rau, Christoph; Schulz, Georg; Müller, Bert

doi:10.1063/1.5085302

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…Combination of these two contrast modes is very useful when the sample has multiple phases, for example highly absorbing components combined with slightly absorbing or purely phase-sensitive materials. [1][2][3][4][5] The third contrast mode that we can obtain from XGTI is the Small Angle X-ray Scattering (SAXS) contrast which manifests itself as reduced visibility of the interference pattern due to presence of the sample. It gives access to spatial information below the pixel size of the detector.…”

Section: Introductionmentioning

confidence: 99%

Development of synchrotron pink beam x-ray grating interferometer at the Diamond Light source I13-2 beamline

Marathe

Storm

Kuppilli

et al. 2019

Developments in X-Ray Tomography XII

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X-ray Grating Talbot Interferometer (XGTI) is already routinely used for quantitative phase contrast imaging of soft tissue samples. XGTI can be realized using various measurement techniques, depending on the X-ray source used, the required spatial resolution and the speed of acquisition. The phase-stepping measurement technique, which is commonly used for XGTI data acquisition, needs multiple acquisitions for a single projection. For fast imaging the Moiré technique, a single-shot technique, is often preferred. However, it requires two gratings which increases the dose on the sample. We have therefore examined the Spatial Harmonic Imaging (SHI) technique which is low-dose and single-shot, using the I13-2 Diamond-Manchester Beamline at Diamond Light Source (DLS). The DLS I13-2 beamline is equipped with a Double Crystal Monochromator (DCM) and a Multi-Layer Monochromator (MLM) to deliver monochromatic beam, which work at the energy bandwidths (ΔE/E) of 10 -4 and 10 -2 , respectively. However, the disadvantage of using these monochromators, especially for fast imaging, is loss of X-ray flux. It has already been shown that XGTI can work with an energy bandwidth (ΔE/E) of 10 -1 . Our aim is to develop a single phase grating interferometer with pink beam from an undulator source, with X-ray mirror optics and multiple absorption filters, to obtain maximum possible flux with sufficient coherence and monochromaticity. We demonstrate performance with optimized beamline parameters for a photon energy of 15 keV with some demonstrative image reconstructions.

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Section: Introductionmentioning

confidence: 99%

Development of synchrotron pink beam x-ray grating interferometer at the Diamond Light source I13-2 beamline

Marathe

Storm

Kuppilli

et al. 2019

Developments in X-Ray Tomography XII

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“…However, it should be noted that the scans were not adapted to absorption imaging. Excellent results have been obtained for imaging of paraffin-embedded brain tissue with absorption-based microCT using optimised setups at synchrotron and also laboratory X-ray sources [Khimchenko et al, 2016a,b;Bikis et al, 2019]. On the other hand, it has been shown that for brain specimens scanned in formalin the phase-contrast signal is nonetheless superior to the absorption image [Schulz et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

X-ray Phase-Contrast Imaging Using Near-Field Speckles

Zdora¹

2021

Springer Theses

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In the last decades, X-ray phase-contrast imaging has proven to be a powerful method for unveiling the inner structure of samples and is capable of visualising even minute density differences. Recently, speckle-based imaging (SBI), the youngest X-ray phase-sensitive technique, has received great interest due to its high sensitivity, quantitative character and relaxed requirements on the setup components and beam properties. This thesis is focussed on the development, experimental optimisation and applications of SBI, with the aim of simplifying its implementation, increasing its flexibility and expanding its potential.For this, a robust, flexible data acquisition and reconstruction approach, the unified modulated pattern analysis (UMPA), was developed, which lifts previous constraints of SBI. UMPA allows for tuning of the sensitivity and spatial resolution by adjusting the scan and reconstruction parameters. It is applicable not only to random speckle, but also periodic interference patterns, bridging the gap and improving the performance of both speckle-and single-grating-based techniques.Following the first demonstration of UMPA, its potential for a range of applications is illustrated in this thesis. It is shown that UMPA can be employed for X-ray optics characterisation to quantify aberrations in the focussing behaviour of X-ray refractive lenses with high precision and accuracy. UMPA phase tomography is applied to the field of biomedical imaging for high-sensitivity three-dimensional (3D) virtual histology of unstained, hydrated soft tissue, giving unprecedented structural and quantitative density information.Further developments of SBI explored in this thesis include the testing of novel customisable speckle diffusers, the extension of SBI to higher X-ray energies for geology and materials science applications, and the demonstration of UMPA at a laboratory X-ray source. These progresses promise new possibilities of SBI for high-sensitivity, robust and high-throughput imaging in previously inaccessible fields and make SBI accessible to a wider range of users in research and industry.

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“…Many experiments are carried out with the in-line phase contrast geometry, but apply also to other techniques. For increased sensitivity the (single-) grating interferometry setup is used [19][20][21][22][23][24] , which is compatible not only with the energy bandwidth provided by the multilayer monochromator but also in pink beam mode 10,11 . Grating interferometry provides in addition phase and small angle scattering information.…”

Section: Operando Imagingmentioning

confidence: 99%

High-throughput micro and nano-tomography

Rau

Marathe

Bodey

et al. 2021

Developments in X-Ray Tomography XIII

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We report about the current and future multi-scale imaging capabilities at the Diamond beamline I13L. Operando and high-throughput imaging is achieved by using either the so-called pink-beam or a multilayer monochromator. The beam modes are compatible with the different techniques covering the micro-to the nano-length scale. In-line phase contrast tomography with up to 300 samples per day is achieved when using an automated sample changer. Fast recording (without the sample changing robot) enables operando studies with a vast variety of dedicated sample environments. For imaging with highest spatial resolution we managed to improve significantly the recording speed of ptycho-tomography, which is now in the order of hours. The Diamond II upgrade planned for 2027 will close the gap in recording times for real and reciprocal space imaging, permitting operando imaging across all length scales. The beamline covers a large range of scientific areas such as materials science, biology and medical research, environmental sciences.

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Sensitivity comparison of absorption and grating-based phase tomography of paraffin-embedded human brain tissue

Cited by 16 publications

References 28 publications

Development of synchrotron pink beam x-ray grating interferometer at the Diamond Light source I13-2 beamline

Development of synchrotron pink beam x-ray grating interferometer at the Diamond Light source I13-2 beamline

X-ray Phase-Contrast Imaging Using Near-Field Speckles

High-throughput micro and nano-tomography

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