Sensitivity determination and resistance risk assessment of <i>Rhizoctonia solani</i> to <scp>SDHI</scp> fungicide thifluzamide

Mu, Wei; Wang, Z.; Bi, Yang; Ni, Xiaoxia; Hou, Yanhua; Zhang, S.; Liu, X.

doi:10.1111/aab.12334

Cited by 28 publications

(20 citation statements)

References 29 publications

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“…The plates were incubated at 25°C in the dark for 2 days, and radial mycelial growth was measured by measuring colony diameter perpendicularly. Mean colony diameter (minus the diameter of the inoculation plug or 5 mm) was measured and expressed as a percentage of growth inhibition (the percentage of growth inhibition determined relative to the fungicide-free check; Mu et al, 2017 ). Concentrations of different fungicides for 40% to 60% mycelial inhibition were selected, and mycelial plug from the periphery of 3-day-old colony was placed on the center of PDA plates amended with the fungicides.…”

Section: Methodsmentioning

confidence: 99%

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

et al. 2020

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The fungicide SYP-14288 has a high efficiency, low toxicity, and broad spectrum in inhibiting both fungi and oomycetes, but its mode of action (MoA) remains unclear on inhibiting fungi. In this study, the MoA was determined by analyzing the metabolism and respiratory activities of Rhizoctonia solani treated by SYP-14288. Wild-type strains and SYP-14288-resistant mutants of R. solani were incubated on potato dextrose agar amended with either SYP-14288 or one of select fungicides acting on fungal respiration, including complex I, II, and III inhibitors; uncouplers; and ATP synthase inhibitors. Mycelial growth was measured under fungicides treatments. ATP content was determined using an ATP assay kit, membrane potential of mitochondria was detected with the JC-1 kit, and respiratory rate was calculated based on the measurement of oxygen consumption of R. solani. A model of metabolic fingerprinting cluster was established to separate oxidation inhibitors and phosphorylation inhibitors. All the results together displayed a clear discrimination between oxidation inhibitors and phosphorylation inhibitors, and the latter inhibited ATP synthase production having or uncoupling activities. Based on the model, SYP-14288 was placed in phosphorylation inhibitor group, because it significantly reduced ATP content and membrane potential of mitochondria while increasing respiratory rate in R. solani. Therefore, the MoA of SYP-14288 on R. solani was confirmed to involve phosphorylation inhibition and possibly uncoupling activity.

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Section: Methodsmentioning

confidence: 99%

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

et al. 2020

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“…The pure active ingredient thifluzamide (96%; Dow AgroSciences Company) was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution (1 × 10 5 μg ml −1 ), which was stored at 4°C in the dark. Fungicide sensitivity assays were conducted according to our previous study 38 . Concentration series of thifluzamide (0, 0.01, 0.025, 0.05, 0.1, and 0.5 μg ml −1 for sensitive isolates; 0, 0.2, 0.5, 1.5, 5, and 20 μg ml −1 for mutants with low to intermediate resistance; and 0, 0.5, 1.5, 5, 20, and 200 μg ml −1 for highly resistant mutants) were used.…”

Section: Methodsmentioning

confidence: 99%

“…The fitness of 14 R. solani was assessed using several criteria including in vitro mycelial growth and biomass, in vitro sclerotia production and germination, and in vivo virulence according to our previous study 38 …”

Section: Methodsmentioning

confidence: 99%

“…Thifluzamide, developed by Dow Agrosciences, belongs to the thiazole–carboxamide group of SDHIs, and has excellent protective and curative activity against rice sheath blight 5,33,34 . Interestingly, although thifluzamide had been used for almost 21 years, no field SDHIs‐resistant R. solani isolates are detected in China according to some recent studies 5,35–38 . According to our previous study, the risk of R. solani developing resistance to thifluzamide is low to moderate, 38 and a point mutation H249Y in protein SDHB was found in thifluzamide‐resistant R. solani isolates 39 .…”

Section: Introductionmentioning

confidence: 97%

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Heterokaryotic state of a point mutation (H249Y) in SDHB protein drives the evolution of thifluzamide resistance in Rhizoctonia solani

Miao

et al. 2020

Pest Management Science

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BACKGROUND Sheath blight, caused by Rhizoctonia solani, can be effectively controlled by application of the succinate dehydrogenase inhibitor thifluzamide. Although the risk of resistance to thifluzamide in R. solani had been reported, the thifluzamide‐resistance mechanism and the evolution of thifluzamide‐resistance in R. solani have not been investigated in detail. RESULTS No differences were found between the sequences of proteins SDHA, SDHC, and SDHD in thifluzamide‐sensitive isolates and thifluzamide‐resistant mutants, but a single point mutation H249Y was found in SDHB. Two different types of thifluzamide‐resistant R. solani mutants were characterized: homokaryotic, carrying only the resistance allele; and heterokaryotic, retaining the wild‐type allele in addition to the resistance allele. The resistance level differed according to the nuclear composition at codon 249 in the sdhB gene. Molecular docking results suggested that the point mutation (H249Y) might significantly alter the affinity of thifluzamide and SDHB protein. Heterokaryotic mutants were able to evolve into a homokaryon when repeatedly cultured on agar media or rice plants in the presence of thifluzamide, but thifluzamide treatment had no effect on the genotypes of homokaryotic mutants or sensitive isolates. CONCLUSION This study showed that H249Y in SDHB protein could cause thifluzamide resistance in R. solani. Fungicide application could promote heterokaryotic mutants to evolve into a homokaryon. © 2020 Society of Chemical Industry

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“…Thifluzamide is mainly used to prevent and control diseases caused by Rhizoctonia spp. of the phylum Basidiomycota [17,18].…”

Section: Introductionmentioning

confidence: 99%

Establishment of baseline sensitivity ofRhizoctonia solanito thifluzamide in corn and its field application

Shang¹,

Yao

et al. 2018

Preprint

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23In recent years, banded leaf sheath blight in corn has become an important disease 24 in corn that seriously affects quality and yield. This paper aims to evaluate the 25 sensitivity of Rhizoctonia solani to thifluzamide in corn, to clarify the effect of seed 26 coating using a thifluzamide suspension agent on safety and physiological indicators 27 and to determine banded leaf sheath blight in corn control effectiveness in the field, 28 thereby providing a basis for the application of thifluzamide suspension agent as a 29 seed coating. In this study, the thifluzamide sensitivity of 102 strains of R. solani in 30 corn in different regions of Shandong was determined using the mycelial growth rate 31 method, and the average half-maximal effective concentration value (EC 50 ) was 32 0.086±0.004 μg/mL. The sensitivity was consistent with a continuous and skewed 33 normal distribution, and the sensitivity distribution frequency exhibited a continuous, 34 unimodal curve, indicating that thifluzamide had strong inhibitory activity on the 35 mycelial growth of R. solani in corn. The impacts of using a thifluzamide suspension 36 agent for seed coating on safety and physiological indicators as well as the control 37 effect in corn were evaluated by combining seed coating, an indoor pot test, and a 38 field trial. The root activities under 24 g a.i. 100 kg -1 seed and 12 g a.i. 100 kg -1 seed 39 were found to increase by 78.01% and 77.40%, respectively, compared with that 40 under the blank control; the chlorophyll content of corn increased most significantly 41 at a dosage of 24 g a.i. 100 kg -1 , which was a 32.32% increase compared to the blank 42 control. Thifluzamide (FS) could significantly increase the hundred-grain weight of 43 corn and the per-plot yield. Among the examined dosages, 24 g a.i. 100 kg -1 seed had 44 the most significant treatment effect, with the hundred-grain weight increasing by 45 12.47% and the yield rate increasing by 15.72% compared to the control in 2016, 46 Simultaneously, the hundred-grain weight increasing by 13.44% and the yield rate 47 increasing by 14.11% compared to the control in 2017. Three dosages of 24% 48 thifluzamide (FS) increased the emergence rate and seedling growth of corn to 49 varying extents. The field control effectiveness against banded leaf sheath blight in 50 corn was best at the dosage of 24 g a.i. 100 kg -1 seed for seed dressing with 51 thifluzamide (FS); in 2016 and 2017, the control effects in the small bell stage, large 52 bell stage, tasseling and pollen-shedding stage, silking stage, milk-ripening stage, and 53 wax-ripening stage were 100%, 66.73%, 52.8%, 67.81%, 68.48%, and 62.68% 54 (2016), respectively, and 74.97%, 63.17%, 50.90%, 53.60%, 61.42%, and 55.88% 55 (2017). These results indicated that thifluzamide had enormous potential for 56 controlling banded leaf sheath blight in corn. 57 59 civilization in recent years, straw burning has been fully prohibited, while straw 60 returning has been widely promoted in various places throughout China. Howeve...

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Sensitivity determination and resistance risk assessment of Rhizoctonia solani to SDHI fungicide thifluzamide

Cited by 28 publications

References 29 publications

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

Metabolic Fingerprinting for Identifying the Mode of Action of the Fungicide SYP-14288 on Rhizoctonia solani

Heterokaryotic state of a point mutation (H249Y) in SDHB protein drives the evolution of thifluzamide resistance in Rhizoctonia solani

Establishment of baseline sensitivity ofRhizoctonia solanito thifluzamide in corn and its field application

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