Sensitivity of an insect mechanoreceptor after destruction of dendritic microtubules by means of vinblastine

Erler, Gernot

doi:10.1007/bf00207705

Cited by 14 publications

(3 citation statements)

References 20 publications

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“…Erler (1983), however, showed (1) that vinblastine degrades the proximal tubules of mechanoreceptive dendrites in Acheta, but not those of the tubular body proper, and (2) that this does not necessarily destroy the electrical activity of the dendrite. Thus, it seems that the micro tubules act in the more passive role of a dendritic cytoskeleton, and that the tubular body supports the dendritic membrane just in the place where it is deformed by the extracellular structures.…”

Section: Cellular Partsmentioning

confidence: 93%

Mechanosensitive and Olfactory Sensilla of Insects

Keil¹,

Steinbrecht²

1984

Insect Ultrastructure

217

169

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Section: Cellular Partsmentioning

confidence: 93%

Mechanosensitive and Olfactory Sensilla of Insects

Keil¹,

Steinbrecht²

1984

Insect Ultrastructure

217

169

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“…The early part of the response is distorted by the stimulus artifact below that in our saline. However, several workers studying mechanoreceptors with methods similar to ours have obtained stable recording conditions using salines containing high Na + and CIand low K + (e.g.. Erler and Thurm 1981;Erler 1983;French 1984;Corfas and Dudai 1990a). We used a high-K + saline for some experiments, and this did not appear to alter the spike frequency response in wild-type or mutant sensilla.…”

Section: Preparation and Recordingmentioning

confidence: 99%

Altered mechanoreceptor response in Drosophila bang-sensitive mutants

Engel

1994

J Comp Physiol A

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Bang-sensitive mutants of Drosophila melanogaster (bas1, bssMW1, eas2, tko25t) display seizure followed by paralysis when subjected to mechanical shock. However, no physiological or biochemical defect has been found to be common to all of these mutants. In order to observe the effects of bang-sensitive mutations upon an identified neuron, and to study the nature of mechanically induced paralysis, we examined the response of a mechanosensory neuron in these mutants. In each single mutant and the double mutant bas1 bssMW1, the frequency of action potentials in response to a bristle displacement was reduced. This is the first demonstration of a physiological defect common to several of the bang-sensitive mutations. Adaptation of spike frequency, cumulative adaptation to repeated stimulation (fatigue) and the time course of recovery from adaptation were also examined. Recovery from adaptation to a conditioning stimulus was examined in two mutants (bas1 and bssMW1), and initial recovery from adaptation was greater in both mutants. Quantification of receptor potentials was complicated by variability inherent in extracellular recording conditions, but examination of the waveform and range of amplitudes did not indicate clear mutant defects. Therefore the differences observed in the spike response may be due to an alteration of the transfer from receptor potentials to action potential production. DNA sequence analysis of tko and eas has indicated that they encode apparently unrelated biochemical products. Our results suggest that these biochemical lesions lead to a common physiological defect in mechanoreceptors.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Thus, the transduction mechanism appears not to depend on the integrity of the microtubule cytoskeleton. This also has been confirmed by Erler (1983) who recorded electrical responses in the hair receptor of the cricket after destruction of the tubular body by visblatine. Interestingly, Gö pfert & Robert (2001) recorded flagellar oscillations in mosquitoes, which failed to elicit electrical responses in the JO.…”

Section: Results (A) Spontaneous Oscillations Of the Flagellummentioning

confidence: 54%

The dynein–tubulin motor powers active oscillations and amplification in the hearing organ of the mosquito

2010

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The design principles and specific proteins of the dynein -tubulin motor, which powers the flagella and cilia of eukaryotes, have been conserved throughout the evolution of life from algae to humans. Cilia and flagella can support both motile and sensory functions independently, or sometimes in parallel to each other. In this paper we show that this dual sensory -motile role of eukaryotic cilia is preserved in the most sensitive of all invertebrate hearing organs, the Johnston's organ of the mosquito. The Johnston's organ displays spontaneous oscillations, which have been identified as being a characteristic of amplification in the ears of mosquitoes and Drosophila. In the auditory organs of Drosophila and vertebrates, the molecular basis of amplification has been attributed to the gating and adaptation of the mechanoelectrical transducer channels themselves. On the basis of their temperature-dependence and sensitivity to colchicine, we attribute the molecular basis of spontaneous oscillations by the Johnston's organ of the mosquito Culex quinquefasciatus, to the dynein -tubulin motor of the ciliated sensillae. If, as has been claimed for insect and vertebrate hearing organs, spontaneous oscillations epitomize amplification, then in the mosquito ear, this process is independent of mechanotransduction.

show abstract

Sensitivity of an insect mechanoreceptor after destruction of dendritic microtubules by means of vinblastine

Cited by 14 publications

References 20 publications

Mechanosensitive and Olfactory Sensilla of Insects

Mechanosensitive and Olfactory Sensilla of Insects

Altered mechanoreceptor response in Drosophila bang-sensitive mutants

The dynein–tubulin motor powers active oscillations and amplification in the hearing organ of the mosquito

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