Sensitivity of bulb tissue section for detection of Fusarium causes Moler disease of shallot

Abstract

Moler caused by Fusarium oxysporum f.sp.cepae is a seed-borne disease, so the disease intensity in the field is depend on the amount of initial inoculum in the infected bulbs as seeds. Therefore, the detection of seed-borne pathogens is important to determine the potential of their transmission. The aim of this research was to evaluate the sensitivity of the bulb tissue of shallot based on the pathogen Infection Index by incubation method on agar media to detect the presence of F. oxysporum f.sp. cepae. The experiment was conducted using a completely randomized design with five replications and three varieties of shallots (Thailand, Bima, and Bauji). Each treatment unit was placed in a Petri’s dish with a diameter of 20 cm, containing 40 mL PDA on which 16 pieces of the tip, middle, and base of the bulb tissues were placed, which were incubated for 7 days. Pathogen Infection Index is a parameter that was observed macroscopically and microscopically. The results showed that the base of the bulbs tissue section of the three varieties tested showed higher sensitivity than the other part to detect the presence of F. oxysporum f.sp. cepae in shallot. It indicates that the basal bulb is the major infection side of the pathogen.

Deteksi Fusarium Patogenik Terbawa Umbi Benih Beberapa Varietas Bawang Merah (Allium cepa L. group aggregatum)

“…Umbi dengan bagian basal plate terdapat gejala nekrosis, merupakan Fusarium patogenik, sedangkan yang tidak nekrosis merupakan Fusarium non patogenik (Gambar 1). Bagian basal plate umbi bawang merah merupakan jaringan yang dapat menunjukkan sensitivitas terhadap adanya infeksi Fusarium dibandingkan dengan dengan bagian lainnya (Widono et al, 2022) . Umbi sebagai bahan perbanyakan tanaman rentan terhadap infeksi patogen dibandingkan dengan biji.…”

Antagonistic activity of Trichoderma sp. against pathogens in the leaves of Allium ascalonicum L.

“…The government has put a lot of effort into optimizing the commodity development. Nonetheless, one of the primary challenges in shallot cultivation is the supply of good quality seeds, where accumulation of pathogens is plausible [2]. The presence of pathogens and its accumulation in shallots could reduce the productivity of its cultivation.…”

Relationship of weather factors on the progress of shallot moler disease in Brebes, Central Java in the rainy and dry seasons: intensity increases in humid and warm air

“…This attack pathogen is difficult to control because FOCe can survive for a long time in soil without a host plant by living as a saprophyte. In addition, FOCe can survive environmental stresses by forming chlamydospores and are always carried by seeds [5][6][7]. Losses or yield losses caused by FOCe vary [3,8,9] and, in susceptible varieties, can be more than 50% [1,3,8,10].…”