Sensitivity of Cercospora beticola populations to fentin-acetate, benomyl and flutriafol in Greece

Karaoglanidis, G. S.; Karadimos, D. A.; Ioannidis, P. M.; Ioannidis, Panayotis

doi:10.1016/s0261-2194(03)00036-x

Cited by 27 publications

(11 citation statements)

References 18 publications

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“…The frequent use of fungicides has consequently increased the chances that resistant populations will develop, which led to an eventual reduction of their efficacy (Karaoglanidis et al, 2003). Moreover, farmers who are faced with problems arising from resistance tend to continue to Wolf and Verreet (2002).…”

Section: Discussionmentioning

confidence: 99%

“…To avoid or delay the evolution of resistant populations, these fungicides are applied with additional protectants, such as chlorothalonil, fentinacetate and fentin-hydroxide (Karaoglanidis and Ioannidis, 2010). The manner in which using multiple treatments of select MBCs and DMIs in the control of C. beticola gradually influenced the development of pathogen resistance has been reported by several authors in North Dakota and Minnesota, USA (Campbell et al, 1998;Weiland and Smith, 1999;Secor et al, 2010) and in Greece (Karaoglanidis et al, 2003).…”

Section: Introductionmentioning

confidence: 93%

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Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management

et al. 2015

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“…In India, yield loss of 80-100% has been attributed to fungal diseases (Anjani et al 2004) and this has affected the income of farmers in terms of foreign exchange. Many in vitro studies have demonstrated that some fungicides restrict or prevent the growth of fungal pathogens (Karaoglanidis et al 2003;Marley and Gbenga 2004). In the literature there are only a few reports about the influence of fungicides on mycelia growth of Fusarium pallidoroseum from Castor plant.…”

Section: Introductionmentioning

confidence: 99%

Effect of six fungicides on sporulation of Fusarium pallidoroseum isolated from castor (Ricinus communis) in Samaru, Nigeria

Mamza¹

2012

IOSRJAVS

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“…Control of the fungus in Greece and other areas with a warm climate and irrigation is based mainly on frequent fungicide applications. However, serious problems have resulted from the extensive appearance of resistant isolates to the intensively used benzimidazoles, organotin fungicides and sterol biosynthesis inhibiting triazoles (Karaoglanidis et al, 2003). Isolates resistant to the benzimidazoles appeared in the early of 1970s resulting in complete failure of disease control (Georgopoulos and Dovas, 1973;Weiland and Halloin, 2001).…”

Section: Introductionmentioning

confidence: 99%

Biological and molecular characterization of laboratory mutants of Cercospora beticola resistant to Qo inhibitors

et al. 2006

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The resistance to strobilurin-related fungicides and its molecular basis in laboratory mutant isolates of Cercospora beticola was investigated. After ultraviolet mutagenesis, mutants with high, moderate or low resistance levels to pyraclostrobin were isolated from a wild-type strain of C. beticola. Fungitoxicity tests on the response of resistant isolates on medium containing pyraclostrobin and salicylhydroxamate (SHAM), a specific inhibitor of cyanide-resistant (alternative) respiration, indicated that the biochemical mechanism of alternative oxidase was not responsible for the reduced sensitivity to pyraclostrobin for half of the mutants. Cross-resistance studies with other inhibitors of the cytochrome bc 1 complex of the mitochondrial respiratory chain showed that the mutation(s) for resistance to pyraclostrobin also reduced the sensitivity of mutant strains to other Qo inhibitors such as azoxystrobin and fenamidone, but not to the Qi inhibitor cyazofamid. No effect of pyraclostrobin-resistant mutation(s) on fungitoxicity of the carboxamide boscalid, the triazoles epoxiconazole and flutriafol and to the benzimidazole benomyl, which affect other cellular pathways or other steps of the respiratory chain, was observed. Study of fitness parameters showed that most mutants had a significant reduction in sporulation and pathogenicity compared to the wild-type parental isolate. However, experiments on the stability of the resistant phenotype did not show a significant reduction of the resistance for half of the mutants when grown for at least four generations on pyraclostrobin-free medium. Molecular analysis of cytochrome b cDNA, isolated from the wild-type and the pyraclostrobin-resistant mutant isolates, revealed two novel amino acid replacements at positions involved in Qo resistance in other species. The glycine (GGT) to serine (AGT) replacement at position 143 (G143S) was found in the isolate with the highly resistant phenotype. The second amino acid change was the replacement of phenylalanine (TTC) by valine (GTC) at position 129 (F129V), which was found in a mutant strain with the moderately resistant phenotype. Four additional mutations located in conserved regions of the mitochondrial cytochrome b gene (I154L, N250D, E256G and V261D) were detected in some mutant isolates of C. beticola but their possible role in Qo-resistance needs further investigation. This is the first study reporting C. beticola strains resistant to Qo inhibitor fungicides due to the biochemical mechanism of target-site modification, resulting from amino acid changes in the mitochondrial cytochrome b gene.

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Sensitivity of Cercospora beticola populations to fentin-acetate, benomyl and flutriafol in Greece

Cited by 27 publications

References 18 publications

Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management

Occurrence of Cercospora beticola populations resistant to benzimidazoles and demethylation-inhibiting fungicides in Serbia and their impact on disease management

Effect of six fungicides on sporulation of Fusarium pallidoroseum isolated from castor (Ricinus communis) in Samaru, Nigeria

Biological and molecular characterization of laboratory mutants of Cercospora beticola resistant to Qo inhibitors

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