With the advent of precision guidance systems, maize (Zea mays L.) farmers in various tillage systems have more options in preplant nutrient banding relative to the intended crop rows. Anhydrous ammonia (NH 3 ) placement during pre-plant application is of interest because of concerns for possible ammonia toxicity to maize seedlings when high NH 3 rates are applied too close to the seed row and the need to improve plant-nitrogen (N) use efficiencies. Field studies were conducted between 2010 and 2012 near West Lafayette, IN, to compare traditional angled (diagonally) vs. precision-guided parallel NH 3 applications (the latter was offset 15 cm from the future row) in no-till and conventional tillage systems. The NH 3 was injected to depths of about 12 cm at N rates of 145 and 202 kg N ha -1 . Maize was planted with additional starter N (20 kg N ha -1 ) within 6 d of NH 3 application. Neither NH 3 application placement resulted in significant maize seedling mortality. Conventional tillage increased mean grain yields across N rates and placement treatments from 10.6 to 11.6 Mg ha -1 . Tillage did not impact reproductive-stage leaf chlorophyll content (SPAD), or whole-plant N content at maturity when NH 3 was parallel applied, but these plant responses were significantly lower in no-till after diagonal application. Lowering the pre-plant N rate to 145 from 202 kg N ha -1 significantly lowered maize whole-plant biomass and N accumulation at maturity with diagonal application, but not when NH 3 was parallel applied. Abbreviations: %Nel, ear-leaf nitrogen concentration, ACRE, Agronomy Center for Research and Education, DM R6 , aboveground biomass production at physiological maturity, GDU°C, Celsius degree based growing degree unit, GY A , grain yield per unit area, HI, harvest index; NH 3 , anhydrous ammonia; NHI, nitrogen harvest index, N total , whole-plant nitrogen uptake at physiological maturity, RTK, real-time kinematic, Sp xx , SPAD readings at xx growth stage, UAN, urea-ammonium-nitrate.Today's agriculture faces two major challenges: (i) satisfying the growing food demand of the ever-expanding world population, while (ii) stabilizing or lowering the associated production risks and environmental pollution. Modern maize production relies heavily on N fertilizer use. Anhydrous ammonia is the most concentrated N source (82% N) and is one of the most widely applied N fertilizers in the last few decades in the United States (IFA, 2013) primarily due to its lower cost per unit of N relative to the cost of other N sources.With the advent of precision guidance systems, maize farmers in various tillage systems have more options in pre-plant nutrient banding relative to the intended crop rows. The first concern in NH 3 placement during pre-plant applications is possible ammonia toxicity to maize seedlings when high NH 3 rates are applied too close in space and time to the future maize rows. Maize farmers in the Midwest region of the United States traditionally apply their pre-plant NH 3 at some degree of angle (i.e...