Sensitivity of<i>Colletotrichum truncatum</i>to Four Fungicides and Characterization of Thiabendazole-Resistant Isolates

Torres-Calzada, Claudia; Tapia-Tussell, Raúl; Higuera‐Ciapara, I.; Martín-Mex, Rodolfo; Nexticapan-Garcéz, Ángel; Pérez‐Brito, Daisy

doi:10.1094/pdis-11-14-1183-re

Cited by 45 publications

(46 citation statements)

References 28 publications

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“…These fungicides are listed in the sanitary registry of plaguicides of the Comisión Federal para la Protección contra Riesgos Sanitarios (Torres-Calzada et al, 2015;COFEPRIS, 2017); azoxystrobin, clorotalonil, mancozeb and tiabendazole fungicides are also used for controling anthracnose in papaya and are registered by the Agencia de Protección al Ambiente (EPA) of the United States of America (Santamaría-Basulto et al, 2011).…”

Section: Controlmentioning

confidence: 99%

“…Dentro del control químico, los fungicidas de la clase benzimidazol y estrobilurinas (actúan inhibiendo la división nuclear y la respiración mitocondrial, respectivamente) se usan ampliamente en campo, estos fungicidas se encuentran vigentes en el registro sanitario de plaguicidas de la Comisión Federal para la Protección contra Riesgos Sanitarios (Torres-Calzada et al, 2015;COFEPRIS, 2017); además, fungicidas como azoxystrobin, clorotalonil, mancozeb y tiabenzadol se usan para el control de antracnosis en papaya y cuentan con registro por parte de la Agencia de Protección al Ambiente (EPA) de los Estados Unidos de América (Santamaría-Basulto et al, 2011).…”

Section: Controlunclassified

“…For example, Torres-Calzada et al (2015) reported C. truncatum isolates resistant to azoxystrobin and tiabendazole, and susceptible to mancozeb and cyprodinil-fludioxonil fungicides. This confirms that the resistance to tiabendazol is caused by a mutation in the β-tubulin gen caused by the replacement of glutamic acid by alanine.…”

Section: Controlmentioning

confidence: 99%

“…Carrillo-Fasio et al (2005) Por otro lado, se ha reportado que el uso continuo de fungicidas sintéticos ha generado resistencia en cepas de Colletotrichum. Por ejemplo, Torres-Calzada et al (2015) determinaron que en C. truncatum existieron aislados resistentes a los fungicidas azoxystrobin y tiabendazol, y susceptibles a los fungicidas mancozeb y cyprodinil-fludioxonil; confirmando que la resistencia a tiabendazol se debe a una mutación en el gen β-tubulina debido a la sustitución de ácido glutámico a alanina. La presencia de cepas resistentes, a algunos productos químicos, ha incrementado el interés por desarrollar otros métodos de control que sean efectivos y seguros para el consumidor y el ambiente, tal es el caso del control biológico.…”

Section: Controlunclassified

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Situación actual de Colletotrichums spp. en México: Taxonomía, caracterización, patogénesis y control

Rojo-Báez

Álvarez-Rodríguez

García‐Estrada

et al. 2017

RMF

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Section: Controlmentioning

confidence: 99%

Section: Controlunclassified

Section: Controlmentioning

confidence: 99%

Section: Controlunclassified

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Situación actual de Colletotrichums spp. en México: Taxonomía, caracterización, patogénesis y control

Rojo-Báez

Álvarez-Rodríguez

García‐Estrada

et al. 2017

RMF

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“…Berdasarkan warna koloni yang bervariasi, Colletotrichum dalam kelompok I ini diidentifikasi sebagai C. gloesporioides seperti yang dikemukakan oleh Rampersad (2011). Tipe morfologi kelompok I yang dicirikan dengan koloni berwarna abuabu sampai abu-abu kehijauan (olive) dan terbentuknya aservulus dengan massa konidia berwarna oranye juga dikuatkan oleh peneliti lain sebagai C. gloeosporioides (MolinaChaves et al 2017;Torres-Calzada et al 2013). Mengingat C. gloeosporioides oleh Weir et al (2012) diketahui sebagai kompleks spesies (species complex), maka Colletotrichum tipe morfologi I yang dalam penelitian ini baru diidentifikasi secara morfologi masih perlu diklarifikasi lebih lanjut secara molekuler.…”

Section: Pembahasanunclassified

Identifikasi Colletotrichum spp. Asal Tanaman Pepaya

Rangkuti

Wiyono

Widodo

2018

J Fitopatol Indones

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ABSTRAKColletotrichum spp. sebagai penyebab penyakit antraknosa merupakan salah satu faktor pembatas produksi pepaya (Carica papaya). Tujuan penelitian ini ialah mengidentifikasi spesies Colletotrichum spp. dari berbagai bagian tanaman pepaya yang menunjukkan gejala antraknosa. Sebanyak 20 galur Colletotrichum spp. berhasil diisolasi, selanjutnya dilakukan pengelompokkan menjadi 3 tipe morfologi berdasarkan kemiripan koloni dan bentuk konidia. Sebanyak 12 galur dari kelompok I dan 3 galur dari kelompok II diidentifikasi sebagai C. gloeosporioides sensu lato berdasarkan morfologi koloni dan bentuk konidia silinder yang kedua ujungnya membulat. Sementara itu 1 galur dari kelompok II secara molekuler diidentifikasi sebagai C. magnum. Empat galur dari kelompok III dengan bentuk konidia melengkung dan berujung runcing, serta membentuk setae pada medium buatan diidentifikasi secara molekuler sebagai C. truncatum. C. gloeosporioides ditemukan pada batang, tangkai daun, dan buah. C. magnum dan C. truncatum masing-masing ditemukan pada tangkai daun dan buah. Selain karakter morfologi dan molekuler, perbedaan respons pertumbuhan koloni terhadap suhu dapat digunakan untuk mengonfirmasi spesies Colletotrichum tersebut. Keberadaan Colletotrichum yang menginfeksi pada bagian batang dan tangkai daun pepaya di pertanaman dan temuan spesies C. magnum dan C. truncatum pada tanaman ini merupakan laporan pertama di Indonesia.Kata kunci: C. gloeosporioides, C. magnum, C. truncatum, morfologi, molekuler ABSTRACT Colletotrichum spp. is known as the causal agent of anthracnose and considered as an important limiting factors on papaya production. The objective of this study was to determine the species of Colletotrichum spp. from various plant parts of papaya (Carica papaya) showing anthracnose symptom. Twenty isolates of Colletotrichum spp. was isolated and were grouped into 3 morphological groups based on colony similarity, conidial morphology, and setae formation. A total of 12 isolates of group I and 3 isolates of group II were identified morphologically as C. gloeosporioides sensu lato based on cylindrical conidia with rounded on both ends and colony morphology. Meanwhile, 1 isolate of group II was molecularly identified as C. magnum. Four isolates of group III with a curved and pointedend conidia, and produced setae on artificial medium were identified molecularly as C. truncatum. C. gloeosporioides was obtained on stem, leaf petiole, and fruit. C. magnum and C. truncatum were only obtained on leaf petiole and fruit, respectively. In addition to morphological and molecular characters, differences in colony growth responses to temperature can be used to distinguish the species of Colletotrichum. To our knowledge the existence of anthracnose symptom on the stems and leaf petioles of papaya in the fields and the discovery of C. magnum and C. truncatum isolated from papaya was the first report in Indonesia.

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Caprylic acid in Vitex mollis fruit and its inhibitory activity against a thiabendazole‐resistant Colletotrichum gloeosporioides strain

López‐Velázquez

Ayón‐Reyna

Vega‐García

et al. 2022

Pest Management Science

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BACKGROUND: Colletotrichum gloeosporioides causes anthracnose in a large number of crops. Synthetic fungicides are employed to prevent this disease, even though their effectiveness and safety is questionable. Thus, effective and innocuous antifungal compounds are proposed as natural alternatives against anthracnose. The hexane fraction of Vitex mollis pulp (HF-VM) reduces anthracnose incidence in papaya fruit; however, the active compounds and antifungal mechanism of HF-VM are unknown. The aims of this study were to characterize the activity of HF-VM sub-fractions (sHF 1 -sHF 7 ) against a thiabendazole-resistant Colletotrichum gloeosporioides strain, identify the chemical components and investigate the mechanism of the most active sub-fraction. RESULTS: The sHF 3 showed the highest inhibitory activity against Colletotrichum gloeosporioides with a minimal inhibitory concentration (MIC) of 0.5 mg mL −1 , whereas thiabendazole (TBZ) had a MIC value higher than 2 mg mL −1 . The gas chromatography-mass spectrometry (GC-MS) analysis showed that the compounds in sHF 3 were methyl 4-decenoate, caprylic acid, and 24-methylencycloartanol. These compounds are rarely found in fruits and are reported for the first time on Vitex species. The purified 24-methylencycloartanol was inactive (MIC > 0.5 mg mL −1 ). In contrast, the commercial standard of caprylic acid presented an elevated activity (MIC = 0.125 mg mL −1 ), indicating that this compound is the main one responsible for the antifungal properties of sHF 3 . Furthermore, the sHF 3 inhibited the spore germination and induced membrane disruption in both the spore and mycelium of Colletotrichum gloeosporioides.CONCLUSION: Vitex mollis fruit is a novel source of antifungal caprylic acid that could be employed as a marker to prepare standardized extracts with antifungal properties.

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Sensitivity ofColletotrichum truncatumto Four Fungicides and Characterization of Thiabendazole-Resistant Isolates

Cited by 45 publications

References 28 publications

Situación actual de Colletotrichums spp. en México: Taxonomía, caracterización, patogénesis y control

Situación actual de Colletotrichums spp. en México: Taxonomía, caracterización, patogénesis y control

Identifikasi Colletotrichum spp. Asal Tanaman Pepaya

Caprylic acid in Vitex mollis fruit and its inhibitory activity against a thiabendazole‐resistant Colletotrichum gloeosporioides strain

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