Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope

Abstract

Objectives: To evaluate the sensitivity of the real-time PCR assay melting curve method in the diagnosis of pulmonary tuberculosis (PTB) by analyzing bronchoalveolar lavage fluid (BALF) obtained from bronchoscopy.
 Methods: A total of 214 PTB patients who were treated at Xuancheng People’s Hospital respiratory and infection department during January 2018 to January 2021 were included in this study. Bronchoscopic bronchoalveolar lavage fluid (BALF) examined by polymerase chain reaction (melting curve method), BALF smear, BALF culture, lipoarabinomannan (LAM) antigen test for diagnosis of tuberculosis (TB) (LAM-TB), acid-fast stain (AFS), and serum adenosine deaminase (ADA) test were conducted respectively to compare their positive predictive values (PPVs).
 Results: Of the 214 patients with confirmed PTB, 84.11% were BALF melting curve method positive, significantly higher than the positive results yielded by other PTB screening tests, i.e., LAM-TB (69.16%), AFS (51.87%), ADA (49.07%), BALF culture (62.15%), and BALF smear (41.12%) (p<0.05, respectively). The PPVs were increased to 92.06%, 93.93%, 92.99%, 95.79%, and 91.12% when BALF melting curve method was performed in combination with LAM-TB, AFS, ADA, BALF culture, and BALF smear, respectively, significantly higher than that produced by BALF melting curve method or the combined use of any two of the non-BALF melting curve method tests (p<0.05, respectively).
 Conclusion: BALF melting curve method is an ideal diagnostic approach to PTB, which is of a higher diagnostic value compared with LAM-TB, AFS, ADA, BALF culture, and BALF smear.
 doi: https://doi.org/10.12669/pjms.38.5.5480
 How to cite this:Wei J, Sun J, Shuai X, Ren J, Chen X. Sensitivity of PCR analysis (melting curve method) in diagnosis of Pulmonary Tuberculosis (PTB) based on Bronchoalveolar Lavage (BAL) by bronchoscope. Pak J Med Sci. 2022;38(5):---------. doi: https://doi.org/10.12669/pjms.38.5.5480
 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Diagnostic value of a nanopore sequencing assay of bronchoalveolar lavage fluid in smear- negative pulmonary tuberculosis

“…Their results thus demonstrated that sequence-based assay accuracy and sensitivity exceeded corresponding standard pathogen detection assay performance indicators [23]. Similarly, Wang et al found that their sequence-based assay was more sensitive than traditional methods for detection of mixed pulmonary infections [24], whereas Chen et al obtained sequence-based test results for BALF specimens collected from patients with severe PTB disease that closely aligned with standard culture method-based results [25]. Here we report for the rst time successful nanopore sequencing assay-based detection of M. tuberculosis in BALF collected from sputum-negative TB patients and demonstrate its value for use in diagnosing suspected PTB cases.…”