Sensitivity of Saturation Transfer Electron Spin Resonance Extended to Extremely Slow Mobility in Glassy Materials

Dries, I.J. van den; Jager, P.A. de; Hemminga, M.A.

doi:10.1006/jmre.1997.1354

Cited by 21 publications

(54 citation statements)

References 17 publications

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“…Ϫ5 s or higher (Dzuba et al, 1984;Roozen et al, 1991;Van den Dries et al, 1998). These R values cannot be determined using Equation 1, because the shapes of the lines in the spectra change due to the appearance of a powder spectrum (Fig.…”

Section: Epr and St-epr Measurementsmentioning

confidence: 99%

“…Here, spectra of CP in anhydrous glycerol were used to construct a calibration curve according to the method of Van den Dries et al (1998). Because the viscosity for anhydrous glycerol is known over a broad temperature range, R of CP in glycerol can be obtained from Equation 2.…”

Section: Epr and St-epr Measurementsmentioning

confidence: 99%

“…From the EPR spectra of the spin probe, R can be assessed (Kumler and Boyer, 1976;Kovarskii et al, 1978;Spielberg and Gelerinter, 1982;Ohta and Kuwata, 1985;Roozen and Hemminga, 1990;Roozen et al, 1991;Dzuba et al, 1993). Whereas R values of 10 Ϫ12 to 10 Ϫ9 s can be calculated from conventional EPR spectra (Knowles et al, 1976), ST-EPR spectroscopy further expands this range to very slow (10 Ϫ6 -10 Ϫ3 s; Hemminga, 1983) and ultra slow (10 Ϫ3 -10 2 s; Van den Dries et al, 1998) molecular motions. ST-EPR spectroscopy has been successfully applied to determine R values of spin probes in sugar glasses (Roozen et al, 1991;Van den Dries et al, 1998) and organic liquids at low temperatures (Ito, 1983).…”

mentioning

confidence: 99%

“…Whereas R values of 10 Ϫ12 to 10 Ϫ9 s can be calculated from conventional EPR spectra (Knowles et al, 1976), ST-EPR spectroscopy further expands this range to very slow (10 Ϫ6 -10 Ϫ3 s; Hemminga, 1983) and ultra slow (10 Ϫ3 -10 2 s; Van den Dries et al, 1998) molecular motions. ST-EPR spectroscopy has been successfully applied to determine R values of spin probes in sugar glasses (Roozen et al, 1991;Van den Dries et al, 1998) and organic liquids at low temperatures (Ito, 1983). In glassy Suc-water and malto-oligosaccharide mixtures, R decreases by several orders of magnitude upon approaching T g (Roozen et al, 1991).…”

mentioning

confidence: 99%

“…These ratios are dependent on the R of the spin probe. To determine the R of CP corresponding to a certain line-height ratio, anhydrous glycerol was used as a reference solvent (this solvent was previously used as a reference in sugar glasses in Roozen et al [1991] and Van den Dries et al [1998]). Although the composition of the cytoplasm in seeds and pollen is not comparable to glycerol, at present the use of glycerol will give the best approximation of the relationship between the line-height ratios and R .…”

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confidence: 99%

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Influence of Water Content and Temperature on Molecular Mobility and Intracellular Glasses in Seeds and Pollen

Buitink

Claessens

Hemminga³

et al. 1998

Plant Physiology

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Although the occurrence of intracellular glasses in seeds and pollen has been established, physical properties such as rotational correlation times and viscosity have not been studied extensively. Using electron paramagnetic resonance spectroscopy, we examined changes in the molecular mobility of the hydrophilic nitroxide spin probe 3-carboxy-proxyl during melting of intracellular glasses in axes of pea (Pisum sativum L.) seeds and cattail (Typha latifolia L.) pollen. The rotational correlation time of the spin probe in intracellular glasses of both organisms was approximately 10 ؊3 s. Using the distance between the outer extrema of the electron paramagnetic resonance spectrum (2A zz ) as a measure of molecular mobility, we found a sharp increase in mobility at a definite temperature during heating. This temperature increased with decreasing water content of the samples. Differential scanning calorimetry data on these samples indicated that this sharp increase corresponded to melting of the glassy matrix. Molecular mobility was found to be inversely correlated with storage stability. With decreasing water content, the molecular mobility reached a minimum, and increased again at very low water content. Minimum mobility and maximum storage stability occurred at a similar water content. This correlation suggests that storage stability might be at least partially controlled by molecular mobility. At low temperatures, when storage longevity cannot be determined on a realistic time scale, 2A zz measurements can provide an estimate of the optimum storage conditions.

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Section: Epr and St-epr Measurementsmentioning

confidence: 99%

Section: Epr and St-epr Measurementsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of Water Content and Temperature on Molecular Mobility and Intracellular Glasses in Seeds and Pollen

Buitink

Claessens

Hemminga³

et al. 1998

Plant Physiology

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133

152

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Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser‐enhanced magnetic resonance imaging

Jebaraj

Utsumi

Benial³

2017

Magnetic Reson in Chemistry

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The electron spin resonance studies were carried out for 2 mm concentration of N-labeled and N-labeled 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl and their deuterated nitroxyl radicals using X-band electron spin resonance spectrometer. The electron spin resonance line shape analysis was carried out. The electron spin resonance parameters such as linewidth, Lorentzian component, signal intensity ratio, rotational correlation time, hyperfine coupling constant and g-factor were estimated. The deuterated nitroxyl radicals have narrow linewidth and an increase in Lorentzian component, compared with undeuterated nitroxyl radicals. The dynamic nuclear polarization factor was observed for all nitroxyl radicals. Upon H labeling, about 70% and 40% increase in dynamic nuclear polarization factor were observed for N-labeled and N-labeled nitroxyl radicals, respectively. The signal intensity ratio and g-value indicate the isotropic nature of the nitroxyl radicals in pure water. Therefore, the deuterated nitroxyl radicals are suitable spin probes for in vivo/in vitro electron spin resonance and Overhauser-enhanced magnetic resonance imaging modalities. Copyright © 2017 John Wiley& Sons, Ltd.

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Spin Label Applications to Food Science

Hemminga¹,

Dries²

2002

Biological Magnetic Resonance

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Sensitivity of Saturation Transfer Electron Spin Resonance Extended to Extremely Slow Mobility in Glassy Materials

Cited by 21 publications

References 17 publications

Influence of Water Content and Temperature on Molecular Mobility and Intracellular Glasses in Seeds and Pollen

Influence of Water Content and Temperature on Molecular Mobility and Intracellular Glasses in Seeds and Pollen

Electron spin resonance studies on deuterated nitroxyl spin probes used in Overhauser‐enhanced magnetic resonance imaging

Spin Label Applications to Food Science

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