Sensitivity of Serologically Classified Strains of Escherichia Coli of Human Origin to the Serum Bactericidal System

Vosti, Kenneth L.; Randall, Eileen L.

doi:10.1097/00000441-197002000-00005

Cited by 90 publications

(57 citation statements)

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“…Clinical isolates of gram-negative bacteria bearing O-Ag vary in serum sensitivity (33,41). This observation has led to some confusion about the role of smooth LPS in protecting cells from complement attack.…”

Section: Resultsmentioning

confidence: 99%

Lipopolysaccharide Size and Distribution Determine Serum Resistance in Salmonella montevideo

1987

J Bacteriol

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The survival of Salmonella montevideo during serum treatment depends on the presence of an 0 antigen (0-Ag) associated with the lipopolysaccharide molecule. In this organism, the 0 antigen is a polysaccharide composed of 0 to more than 55 subunits, each containing 4 mannose residues together with glucose and n-acetylglucosamine. We used a mutant strain of S. montevideo that smooth, enteric gram-negative bacteria is efficient, and the bactericidal C5b-9 complex is formed on the surface of such smooth cells (13). However, in contrast to the stable, hydrophobic interaction of C5b-9 with rough cells, the C5b-9 complex is bound by weak hydrophilic interactions to smooth cells and is released from the surface (14). Consequently, it is not bactericidal. We and others (13,14,36) have suggested that C5b-9 complexes are shed because complement activation takes place on long O-Ag side chains and that C5b-9 complexes formed are sterically inhibited from inserting into hydrophobic membrane domains. In a recent paper (12)

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Section: Resultsmentioning

confidence: 99%

Lipopolysaccharide Size and Distribution Determine Serum Resistance in Salmonella montevideo

1987

J Bacteriol

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“…The serum bactericidal test was performed in a microtitre system using a disposable Uwell trays (1,3,5). Before use the sera were sterilised using a 0.45ul membrane filter as described by Baker et al (23).…”

Section: Performance Of Bacterial Assaymentioning

confidence: 99%

“…A sterilised standard wire loops were then used to deliver a drop of mixture from each well unto blood agar plates. The plates were incubated aerobically for 18hours at 37°C and the colony count was obtained as earlier described (1,3,5). Briefly, the culture plates were opened and a magnifying hand lens was raised above it and looking through the lens count was made .…”

Section: Performance Of Bacterial Assaymentioning

confidence: 99%

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Bactericidal activity of human sera against <i> Salmonella Typhi</i>and <i>Salmonella paratyphi</i> A,B,C

Igumbor¹,

Osayande²

2009

E Af Med Jrnl

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Objectives: To determine the sensitivity of Salmonella typhi and paratyphi A,B,C, to normal human blood serum; and assess the role of blood groups (ABO system), Complement and Immunoglobulin in the resistance or susceptibility of Salmonella typhi and Salmonella paratyphi A,B,C infections. Design: Cross sectional study. Subjects: Ninety-six apparently healthy males and females volunteers, aged 18-24 years. Main outcome measures: Resistance of Salmonella typhi and Salmonella paratyphi A,B,C infections may be blood group and immune status dependent. Results: Blood group B was most resistant to Salmonella typhi and Salmonella paratyphi A,B,C while blood group O showed least resistance (51.9%) and (22.2%) for Salmonella typhi and Salmonella paratyphi A,B,C. There was no difference in resistance pattern when blood was pooled in respect to their group types. Age or sex of the blood donors had no effect on the bactericidal activity of the sera. Conclusion: Blood group is an important factor in the susceptibility or resistance of an individual to Salmonella typhi and paratyphi infections. Individuals of blood group O are likely to be more susceptible to infections caused by Salmonella typhi and paratyphi A,B,C.

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“…Resistance to these bactericidal effects has since been shown to be correlated with virulence of gram-negative bacteria in various infections, including burn infections (Lowbury and Ricketts, 1957), urinary tract infections (Vosti and Randall, 1970;Gower et al, 1972;Olling, 1977;Bjorksten and Kaijser, 1978;Hughes, Phillips and Roberts, 1982), endocarditis (Archer and Fekety, 1976;Durack and Beeson, 1977) and with the severity of bacteraemia (McCabe et al, 1978;Clumeck et al, 1982). Most studies have been conducted with Escherichia coli isolates from various sources but serum resistance has also been reported among other genera of Enterobacteriaceae including Klebsiella, Serratia and Proteus (Roantree and Rantz, 1960;Olling, 1977;Clumeck et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Contribution of the traT Gene to Serum Resistance Among Clinical Isolates of Enterobacteriaceae

Kanukollu¹,

Bieler²,

Hull³

et al. 1985

Journal of Medical Microbiology

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SUMMARY. Antimicrobial resistance plasmids containing the traT gene confer resistance to serum bactericidal activity upon some laboratory strains of Escherichia coli. We have examined the DNA of Enterobacteriaceae from human extraintestinal infections to determine the frequency of traT-like genes. DNA sequences homologous with traT were found among 58% of Escherichieae but among none of the Klebsielleae or Proteeae tested and were found as frequently among serum-sensitive E. coli as among serum-resistant strains. Sequences related to traT were always associated with large plasmids. The potential contribution of traT-containing plasmids to serum resistance of clinical isolates is discussed.

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Sensitivity of Serologically Classified Strains of Escherichia Coli of Human Origin to the Serum Bactericidal System

Cited by 90 publications

References 0 publications

Lipopolysaccharide Size and Distribution Determine Serum Resistance in Salmonella montevideo

Lipopolysaccharide Size and Distribution Determine Serum Resistance in Salmonella montevideo

Bactericidal activity of human sera against <i> Salmonella Typhi</i>and <i>Salmonella paratyphi</i> A,B,C

Contribution of the traT Gene to Serum Resistance Among Clinical Isolates of Enterobacteriaceae

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