Sensitivity of Tilapia (<i>Oreochromis mossambicus</i>
) to <i>Clostridium botulinum</i>
 toxins

Lalitha, K.V.; Gopakumar, K.

doi:10.1046/j.1365-2109.2001.00616.x

Cited by 7 publications

(7 citation statements)

References 8 publications

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“…In contrast, a reduction of color intensity has been reported in BoNT/E-intoxicated tilapia (Lalitha and Gopakumar, 2001) and rainbow trout (Cann and Taylor, 1982) but was not observed in the rainbow trout intoxicated in this study.…”

Section: Discussioncontrasting

confidence: 52%

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Toxicity of Clostridium Botulinum Type E Neurotoxin to Great Lakes Fish: Implications for Avian Botulism

Yule

Barker

Austin

et al. 2006

Journal of Wildlife Diseases

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ABSTRACT:Since 1999, large-scale mortalities of fish-eating birds have been observed on the Great Lakes, and more specifically on Lake Erie. Type E botulism has been established as the primary cause of death. The mechanism of type E botulism exposure in fish-eating birds is unclear. Given that these birds are thought to eat live fish exclusively, it seems likely that their prey play a key role in the process, but the role of fish as potential transport vectors of botulinum neurotoxin type E (BoNT/E) to birds has not been adequately investigated. Between June 2003 and April 2004 a methodological model for exposing fish to Clostridium botulinum was developed and used to compare the sensitivity of rainbow trout (Oncorhynchus mykiss), round goby (Neogobius melanostomas), walleye (Stizostedion vitreum), and yellow perch (Perca flavescens) to four doses (0, 800, 1,500, and 4,000 Mouse Lethal Doses) of Clostridium botulinum type E neurotoxin. Each fish species expressed unique changes in both behavior and skin pigmentation prior to death. Yellow perch survived significantly longer (P,0.05) than the three other species at all toxin treatments. Results of this study suggest that live fish can represent a significant vector for transfer of BoNT/E to birds.

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Section: Discussioncontrasting

confidence: 52%

“…This orientation has been reported in tilapia exposed to BoNT/E (Lalitha and Gopakumar, 2001). Similarly, coho salmon exposed to BoNT/E moved toward the surface of the water, following which they would sink to the bottom as though they were ''tail heavy'' (Eklund et al, 1982(Eklund et al, , 2004.…”

Section: Discussionmentioning

confidence: 85%

Toxicity of Clostridium Botulinum Type E Neurotoxin to Great Lakes Fish: Implications for Avian Botulism

Yule

Barker

Austin

et al. 2006

Journal of Wildlife Diseases

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“…comm.). In another study, C. botulinum types A, B, C, D, and E were shown to be toxic to tilapia when injected intraperitoneally, with the fish being most sensitive to type E (Lalitha & Gopakumar 2001).…”

Section: Introductionmentioning

confidence: 98%

Susceptibility of coho salmon, Oncorhynchus kisutch (Walbaum), to different toxins of Clostridium botulinum

Eklund¹,

Poysky

Paranjpye

et al. 2004

Aquac Research

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Coho salmon Oncorhynchus kisutch (Walbaum), held at 15°C were tested for their susceptibility to toxins of proteolytic and nonproteolytic Clostridium botulinum types A, B, C1, C2, D, E, F, and G administered by the oral and intraperitoneal (i.p.) routes. By the oral route, the fish were most susceptibile to type E neurotoxin, which was lethal at a dose equivalent to 90 mouse intraperitoneal minimum lethal doses (MLDs). The oral lethal dose increased to 2000 MLD for nonproteolytic and proteolytic type F neurotoxins, but the toxin types A, B, and C1 were not lethal to fish at 2000 MLD and type D was not lethal at 20 000 MLD (highest titre tested). The fish were not susceptible to 200 MLD (the highest titres tested) of type G neurotoxin or C2 cytotoxin. By the i.p. route, all of the toxins except type G were lethal to coho salmon. Type E neurotoxin was the most toxic at a level of one‐half the mouse MLD. Coho salmon held at temperatures ranging from 1 to 20°C were sensitive to type E neurotoxin by both the oral and i.p. routes. As the temperature decreased the fish became more resistant to type E neurotoxin by the oral route, but the i.p. dose remained one‐half the mouse MLD at all temperatures.

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“…Botulism is a paralytic disease mainly caused by C. botulinum and rarely C. boturicum and C. barrati [1]. The disease is more evident in children under one year of age because there is more chance to germinate spore in the gastro-intestinal tract (GIT) and so productions of more exotoxins [2].…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Clostridium botulinum in Four Species of Fish in the Persian Gulf

Sarvestani¹,

Poormontaseri²,

Fazeli³

et al. 2016

Int Arch Med

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Background: Food poisoning (FP) caused by Clostridium botulinum is the most serious feature of FP in man consuming the contaminated food stuffs specifically seafood. The occurrence of the contamination to different species of the bacteria in four species of fish in Persian Gulf was investigated using selective culture and multiplex PCR (mPCR) assays. Methods: The samples were initially enriched in cooked meat broth for 7 days at 30 °C in an anaerobic condition followed by subculture onto blood agar. DNA of the samples were then extracted for further mPCR assay using three species-specific pair of primers to amplify the 782, 205 and 389 bp fragments corresponding to the A, B and E types of the microorganism (MO). Results: Out of 120 specimens, C. botulinum was detected in 5 samples (4.2%). Altogether, five and two samples were respectively positive in the mPCR and culture assays which were significantly different (p < 0.05). Conclusions: No evidence of type E was shown; which, was possibly resulted from the limitation of our study. Our results have suggested a reliable molecular assay to employ in the food Laboratories for rapid identification and differentiation of various kinds of C. botulinum.

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Sensitivity of Tilapia (Oreochromis mossambicus ) to Clostridium botulinum toxins

Cited by 7 publications

References 8 publications

Toxicity of Clostridium Botulinum Type E Neurotoxin to Great Lakes Fish: Implications for Avian Botulism

Toxicity of Clostridium Botulinum Type E Neurotoxin to Great Lakes Fish: Implications for Avian Botulism

Susceptibility of coho salmon, Oncorhynchus kisutch (Walbaum), to different toxins of Clostridium botulinum

Occurrence of Clostridium botulinum in Four Species of Fish in the Persian Gulf

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