Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: Relationship with Fas, caspase-8, and Bid

Hougardy, Brigitte M.T.; Zee, Ate G.J. van der; Heuvel, Fiona A.J. van den; Timmer, Tineke; Vries, Elisabeth G.E. de; Jong, Steven de

doi:10.1016/j.ygyno.2005.01.036

Cited by 39 publications

(48 citation statements)

References 45 publications

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“…HeLa was used as a positive control (Hougardy et al, 2005). As shown in Figure 4, protein products corresponding to the predicted molecular mass of B36 kDa for FasL were detected in HeLa, GIST882, and in all six GISTs.…”

Section: Fas and Fasl Expression In Gist By Western Blot Analysismentioning

confidence: 97%

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

et al. 2008

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Although the tyrosine kinase inhibitor imatinib has been shown to be an active agent in patients with gastrointestinal stromal tumours (GIST), complete remissions are almost never seen and most patients finally experience disease progression during their course of treatment. An alternative therapeutic option is to target death receptors such as Fas. We showed that a panel of imatinib-sensitive (GIST882) and imatinib-resistant (GIST48, GIST430 and GIST430K-) cell lines expressed Fas. MegaFasL, a recently developed hexameric form of soluble Fas ligand (FasL), appeared to be an active apoptosis-inducing agent in these cell lines. Moreover, MegaFasL potentiated the apoptotic effects of imatinib. Immunohistochemical evaluations, in 45 primary GISTs, underscored the relevance of the Fas pathway: Fas was expressed in all GISTs and was expressed strongly in 93%, whereas FasL was expressed at moderate and strong levels in 35 and 53% of GISTs, respectively. Fas and FasL expression were positively correlated in these primary GISTs, but there was no association between Fas or FasL expression and primary site, histological subtype, tumour size, mitotic index, risk classification, and KIT mutation status. The abundant immunohistochemical Fas and FasL expression were corroborated by western blot analysis. In conclusion, our data implicate Fas as a potential therapeutic target in GIST.

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Section: Fas and Fasl Expression In Gist By Western Blot Analysismentioning

confidence: 97%

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

et al. 2008

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“…Transcripts of the gene for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as an internal control. Sequences of the PCR primers were as follows: CuZn-SOD forward 5'-AAG GCC GTG TGC GTG CTG AA-3', reverse 5'-CAG GTC TCC AAC ATG CCT CT-3' (246 bp product) (El Mouatassim et al, 1999); CAT forward 5'-GCA GAT ACC TGT GAA CTG TC-3', reverse 5'-GTA GAA TGT CCG CAC CTG AG-3' (229 bp product) (El Mouatassim et al, 1999); and, GAPDH forward 5'-GAC CCC TTC ATT GAC CTC AAC T-3', Reverse 5'-GTT TGT GAT GGG TGT GAA CCA-3' (200 bp product) (Hougardy et al, 2005). The PCR protocol consisted of denaturation at 94 °C for 1 min, 35 cycles of denaturation at 94 °C for 45 s, annealing at 56 °C (CuZn-SOD) or 55 °C (CAT) for 1 min, extension at 72 °C for 1 min, and final extension at 72 °C for 15 min.…”

Section: Reverse Transcription-polymerase Chain Reaction (Rt-pcr) Anamentioning

confidence: 99%

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo

Wang

Sheng

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:The present study is designed to investigate the protection by ferulic acid against the hepatotoxicity induced by diosbulbin B and its possible mechanism, and further observe whether ferulic acid augments diosbulbin Binduced anti-tumor activity. The results show that ferulic acid decreases diosbulbin B-increased serum alanine transaminase/aspartate transaminase (ALT/AST) levels. Ferulic acid also decreases lipid peroxide (LPO) levels which are elevated in diosbulbin B-treated mice. Histological evaluation of the liver demonstrates hydropic degeneration in diosbulbin B-treated mice, while ferulic acid reverses this injury. Moreover, the activities of copper-and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) are decreased in the livers of diosbulbin B-treated mice, while ferulic acid reverses these decreases. Further results demonstrate that the mRNA expressions of CuZn-SOD and CAT in diosbulbin B-treated mouse liver are significantly decreased, while ferulic acid prevents this decrease. In addition, ferulic acid also augments diosbulbin B-induced tumor growth inhibition compared with diosbulbin B alone. Taken together, the present study shows that ferulic acid prevents diosbulbin B-induced liver injury via ameliorating diosbulbin B-induced liver oxidative stress injury and augments diosbulbin B-induced anti-tumor activity.

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“…6,7 The other is IFN-␥, which directly increases Fas expression. 8,9 Both are highly expressed in activated CD4 ϩ T cells. 10 However, the possibility that the proapoptotic effects of these 2 molecules might be shadowed by that of FasL highly expressed on CIKs should not be excluded in Fas-sensitive Raji cells.…”

Section: Introductionmentioning

confidence: 99%

CD4⁺T Cells in CIKs (CD4⁺CIKs) Reversed Resistance to Fas-Mediated Apoptosis Through CD40/CD40L Ligation Rather Than IFN-γStimulation

Zhang

Jiang

et al. 2008

Cancer Biotherapy and Radiopharmaceuticals

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Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: Relationship with Fas, caspase-8, and Bid

Cited by 39 publications

References 45 publications

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo

CD4⁺T Cells in CIKs (CD4⁺CIKs) Reversed Resistance to Fas-Mediated Apoptosis Through CD40/CD40L Ligation Rather Than IFN-γStimulation

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Sensitivity to Fas-mediated apoptosis in high-risk HPV-positive human cervical cancer cells: Relationship with Fas, caspase-8, and Bid

Cited by 39 publications

References 45 publications

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

Abundant Fas expression by gastrointestinal stromal tumours may serve as a therapeutic target for MegaFasL

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo

CD4+T Cells in CIKs (CD4+CIKs) Reversed Resistance to Fas-Mediated Apoptosis Through CD40/CD40L Ligation Rather Than IFN-γStimulation

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Product

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CD4⁺T Cells in CIKs (CD4⁺CIKs) Reversed Resistance to Fas-Mediated Apoptosis Through CD40/CD40L Ligation Rather Than IFN-γStimulation