Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis

Hashimoto, Mitsuru; Nigi, Hideo; Sakaguchi, Masahiro; Inouye, Sakae; Imaoka, Koichi; Miyazawa, Hiroshi; Taniguchi, Yoshifumi; Kurimoto, Masashi; Yasueda, Hiroshi; Ogawa, Teruhiro

doi:10.1111/j.1365-2222.1995.tb00027.x

Cited by 89 publications

(51 citation statements)

References 7 publications

(5 reference statements)

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“…It has been reported that patients with Japanese cedar pollinosis exhibit a high titer allergen-specific IgE response (38). Allergenspecific IgE antibodies have been shown to play a major biological role for the induction of pollen-associated allergic responses (38, 39).…”

Section: Discussionmentioning

confidence: 99%

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

206

137

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Peptide immunotherapy using multiple predominant allergen-specific T cell epitopes is a safe and promising strategy for the control of type I allergy. In this study, we developed transgenic rice plants expressing mouse dominant T cell epitope peptides of Cry j I and Cry j II allergens of Japanese cedar pollen as a fusion protein with the soybean seed storage protein glycinin. Under the control of the rice seed storage protein glutelin GluB-1 promoter, the fusion protein was specifically expressed and accumulated in seeds at a level of 0.5% of the total seed protein. Oral feeding to mice of transgenic rice seeds expressing the T cell epitope peptides of Cry j I and Cry j II before systemic challenge with total protein of cedar pollen inhibited the development of allergen-specific serum IgE and IgG antibody and CD4 ؉ T cell proliferative responses. The levels of allergen-specific CD4 ؉ T cell-derived allergy-associated T helper 2 cytokine production of IL-4, IL-5, and IL-13 and histamine release in serum were significantly decreased. Moreover, the development of pollen-induced clinical symptoms was inhibited in our experimental sneezing mouse model. These results indicate the potential of transgenic rice seeds in production and mucosal delivery of allergen-specific T cell epitope peptides for the induction of oral tolerance to pollen allergens.Japanese cedar pollinosis ͉ peptide immunotherapy ͉ seed-specific expression

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Section: Discussionmentioning

confidence: 99%

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Takagi

Hiroi

Yang

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

206

137

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“…Two major allergenic proteins, Cry j 1 and Cry j 2, have been isolated [1, 2]and both are thought to be important in the immunopathology of Japanese cedar pollinosis, since IgE specific to Cry j 1 was detected in up to 95% of the patients, while that to Cry j 2 in about 70% [2, 3, 4, 5]. Determination of primary structures of the Cry j 1 [6]and Cry j 2 [3, 7]was followed by the identification of multiple T–cell epitopes in both sequences [8, 9, 10].…”

Section: Introductionmentioning

confidence: 99%

Peptide Specificity, HLA Class II Restriction, and T–Cell Subsets of the T–Cell Clones Specific to Either Cry j 1 or Cry j 2, the Major Allergens of Japanese Cedar (Cryptomeria japonica) Pollen

Sone¹,

Morikubo²,

Shimizu³

et al. 1999

Int Arch Allergy Immunol

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Background: Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T–cell epitopes in both allergens and their role in induction of T–cell subsets are not well known. Methods: CD4+ T (Th)–cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were generated. HLA class II restrictions were determined by their reactivity to the T–cell epitope in the presence of antigen presenting cells sharing matched types. Interleukin (IL)–2, interferon–γ, IL–4, and IL–5 contents in the supernatants of TCCs were estimated using enzyme immunoassay. Results: Peripheral blood mononuclear cells (PBMC) from patients induced proliferation with 100 μg/ml Cry j 1 or 3–10 μg/ml rCry j 2 stimulation. T–cell epitopes in Cry j 1 were presented to Th cells by the gene products of DRA1*01/DRB1*0901, DRA1*01/DRB5*0101, DQA1* 0102/DQB1*0602, and DPA1*01/DPB1*0501; those in Cry j 2 were restricted by DRA1*01/DRB1*0901, DRA1* 01/DRB1*1501, DRA1*01/DRB4*01, DRA1*01/DRB5* 0101, DQA1*0102/DQB1*0602, DPA1*01/DPB1*0201, and DPA1*01 and *0202/DPB1*0501. Type 2–like cells were preferentially induced in Cry j 1 stimulation, while an almost equal number of type 2– and type 1–like cells was induced in rCry j 2. Conclusions: No clear correlation existed between peptide specificity, HLA class II restriction and induction of Th–cell subsets, suggesting that the requirement of different dose of Cry j 1 or Cry j 2 to induce proliferation in PBMC may lead to distinguishable difference in induction of Th subsets between TCCs specific to Cry j 1 and Cry j 2.

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“…The following allergens have been characterized in this source: Cry j 1, a 45-50 kDa protein, a pectate lyase, is considered a major allergen (Yasueda et al 1983;Taniai et al 1988;Griffith et al 1993;Sone et al 1994;Taniguchi et al 1995;Hashimoto et al 1995; (Ibrahim et al 2010b), and Cry j LTP, a Lipid Transfer Protein (Ibrahim et al 2010c). Moreover, a number of other antigenic proteins have been isolated but not characterized, including proteins of 7, 15 and 20 kDa (Matsumura et al 2006).…”

Section: Japanese Cedar (Cryptomeria Japonica Synonym: Cupressus Japmentioning

confidence: 99%

Pollen Allergenicity is Highly Dependent on the Plant Genetic Background: The “Variety”/“Cultivar” Issues

Alché¹,

Zafra²,

Jiménez-López³

et al. 2012

Current Insights in Pollen Allergens

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Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis

Abstract: Cry j II is an as important a major allergen as Cry j I.

Cited by 89 publications

References 7 publications

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses

Peptide Specificity, HLA Class II Restriction, and T–Cell Subsets of the T–Cell Clones Specific to Either Cry j 1 or Cry j 2, the Major Allergens of Japanese Cedar (Cryptomeria japonica) Pollen

Pollen Allergenicity is Highly Dependent on the Plant Genetic Background: The “Variety”/“Cultivar” Issues

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