1997
DOI: 10.1016/s0304-4165(96)00106-7
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Sensitization of skin fibroblasts to UVA by excess iron

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Cited by 40 publications
(22 citation statements)
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“…Several lines of evidence strengthen this model. Firstly, UVA-mediated membrane damage in human skin fibroblasts could be prevented in cells pre-treated with iron chelators, and conversely could be enhanced in ironloaded cells (Morliere et al, 1991(Morliere et al, , 1997Punnonen et al, 1991;. Secondly, UVA-mediated activation of NF-kB appeared to be correlated with membrane damage, since it could be prevented by the membrane antioxidants BHT and a-tocopherol acetate as previously demonstrated by and as shown in Fig 6, this study.…”
Section: Figuresupporting
confidence: 80%
“…Several lines of evidence strengthen this model. Firstly, UVA-mediated membrane damage in human skin fibroblasts could be prevented in cells pre-treated with iron chelators, and conversely could be enhanced in ironloaded cells (Morliere et al, 1991(Morliere et al, , 1997Punnonen et al, 1991;. Secondly, UVA-mediated activation of NF-kB appeared to be correlated with membrane damage, since it could be prevented by the membrane antioxidants BHT and a-tocopherol acetate as previously demonstrated by and as shown in Fig 6, this study.…”
Section: Figuresupporting
confidence: 80%
“…4). In contrast, vitamin E not only inhibits lipid peroxidation in UVAexposed fibroblasts [9], but also impedes membrane injury [9,10,30] and can partly restore cell viability after UVA irradiation. Indeed, the exposure of cultured human skin fibroblasts from four different donors to 60 J/cm 2 dropped the colony-forming ability to 5.0Ϯ 0.9, 9.6Ϯ 4.3, 7.3 Ϯ 1.3 and 19.4Ϯ 4.9% of the control, respectively (data not shown).…”
Section: Discussionmentioning
confidence: 99%
“…The neutral red uptake assay was performed according to the method of Lasarow [29] and as earlier detailed [30]. Absorbances measured at 550 nm in a 96-well microplate reader, are the mean ϮSD of triplicates and are normalized to the absorbance of untreated sham-irradiated cells taken as 100.…”
Section: Methodsmentioning
confidence: 99%
“…These low-molecular-mass complexes can initiate free radical damage as they supply iron ions in a form capable of accelerating free radical reactions and lipid peroxidation (25). In this regard, iron sensitizes cultured human skin fibroblasts to the ultraviolet A (UVA) radiation when cells are treated with low molecular mass complexes or exposed to high ferritin and holotransferrin concentrations with evidence of increased membrane lipid peroxidation and cytotoxicity (26) and activation of MAP kinases (27). The present study, showing that the extracellular space is the major site of excess iron deposition in the skin of patients with HH (Figs IB and 2), might provide evidence about the lack of notable UVA-induced skin sensitization.…”
Section: Discussionmentioning
confidence: 99%