Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Hirata, Hirokuni; Yoshida, Naruo; Watanabe, Mineaki; Sugiyama, Kumiya; Arima, Masafumi; Ishii, Yoshiki

doi:10.1016/j.alit.2014.08.008

Cited by 4 publications

(5 citation statements)

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“…The minority of patients with wasp and hornet allergy (8/39) had sIgE‐reactivity to rVes v 5 and rVes v 1. The results of several studies have confirmed that rVes v 5 is sufficient in most patients for serological confirmation of wasp or hornet allergy 15,26,39 . When a differential diagnosis between wasp and hornet allergy is required a history of insect sting allergy is very important, in addition of sIgE‐reactivity to rVes v 5.…”

Section: Discussionmentioning

confidence: 93%

“…The results of several studies have confirmed that rVes v 5 is sufficient in most patients for serological confirmation of wasp or hornet allergy. 15,26,39 When a differential diagnosis between wasp and hornet allergy is required a history of insect sting allergy is very important, in addition of sIgE-reactivity to rVes v 5. In addition, the sensitization rate of honeybee allergic patients to rApi m 1 is 57% to 83.3%, and sensitization rate of wasp allergic patients to rVes v 5 is 86.55%-96.7% measured by ImmunoCAP.…”

Section: Discussionmentioning

confidence: 99%

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Molecular diagnostics and inhibition of cross‐reactive carbohydrate determinants in Hymenoptera venom allergy

Jovanovic

Perić-Popadić

Djuric

et al. 2023

Clinical & Translational All

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Background The composition of venom extracts, cross‐reactive carbohydrate determinants (CCD) and the component‐resolved diagnostics (CRD) are important fields of investigation. IgE‐reactivity to CCD complicates the interpretation of IgE to Hymenoptera venoms, especially in patients with multiple‐positivity. We analyzed the clinical importance of CRD and CCD‐inhibition for selection of allergens for venom immunotherapy (VIT). Methods In 71 patients, we measured specific IgE (sIgE) to honeybee venom (HBV), wasp venom (WV), hornet venom (HV), CCD, and recombinant allergens: phospholipase A2 (rApi m 1), hyaluronidase (rApi m 2), icarapin (rApi m 10), antigen 5 (rVes v 5), and phospholipase A1 (Immunoblot). In 29/71 HBV/WV/HV/CCD‐positive patients CCD‐inhibition was performed. According to CRD and CCD‐inhibition, we identified true sensitization and defined groups of multiple‐positive patients who needed CCD‐inhibition before starting VIT. Results sIgE‐rApi m 1, sIgE‐rApi m 2, and sIgE‐rApi m 10 were detected in 65.7%, 68.4%, and 58%, respectively. In HBV allergic patients, CRD sensitivity was 86.8%. In WV allergic patients, sensitivity of sIgE‐rVes v 5 was 94%. True multiple‐sensitization was found in 44.8% of HBV/WV/HV/CCD‐positive patients after CCD‐inhibition. Patients with multiple venom‐ and CCD‐positivity had more frequent severe allergic reactions (p < 0.001). CCD‐inhibition was helpful in HBV/WV/HV/CCD‐positive patients who were negative to all tested recombinant honeybee allergens. Persistence of HBV‐positivity after CCD‐inhibition requires CRD to other honeybee recombinant allergens. Conclusion CRD, using a profile of five most important recombinant allergens and CCD, has a high sensitivity for the diagnosis of venom allergy, especially in patients positive to several venom extracts. CRD and CCD‐inhibition are helpful to reveal the clinically relevant, true sensitization and improve the selection of venoms for long‐lasting VIT.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Molecular diagnostics and inhibition of cross‐reactive carbohydrate determinants in Hymenoptera venom allergy

Jovanovic

Perić-Popadić

Djuric

et al. 2023

Clinical & Translational All

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“…Elevated sIgE to CCDs explain the finding of increased sIgE levels against the native bee, wasp and hornet venoms (Table 1) detected by the ELISA 2,3,7,15 . A high percentage, even up to 93% of subjects sensitized to the hornet venom have elevated sIgE to rVes v5 [16][17][18] . Crossreactivity that occurs between the venoms of different Vespidae (Vespula vulgaris, Vespa crabo -European hornet) is strong, due to the similarities of venom composition and structure of allergens.…”

Section: Discussionmentioning

confidence: 98%

Triple IgE-positivity to hornet, wasp and bee venom in the patient with anaphylaxis: Diagnostic and therapeutic approach

et al. 2019

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Introduction. Triple-positivity (TP) or double-positivity (DP) for serum-specific immunoglobulin E (sIgE) antibodies against hornet venom (HV), wasp venom (WV) and/or honeybee venom (BV) causes significant problem in a selection of appropriate venom immunotherapy. However, DP/TP can be caused by cross-reactions resulting either from partial sequence identity of protein allergens in the venoms, or may be related to cross-reacting carbohydrate determinants (CCDs). Case report. A 60-year-old man was stung by a wasp and two days later by hornet. In both cases, within 15 minutes he developed hypotension and generalized urticaria and he was successfully treated with epinephrine, corticosteroids and fluids. After eight weeks, the examination revealed the negative skin prick test for all three venoms, but the sIgE-determination (ELISA, Biopharm) showed triple sensitization to native BV (0.55 IU/mL), WV (3.35 IU/mL) and HV (0.37 IU/mL). He was receiving the venom immunotherapy with venom mixtures for one year.

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“…The authors did not specify the species responsible for the reaction nor the species of hornet used for the sIgE determination. Some 92.6% of these patients presented sIgE to rVes v 5, and the authors concluded that Ves v 5 allergens are the most potent allergens in hornet venom 59 . These results are not surprising, given that antigen 5 was proven to be a dominant allergen in vespid venoms other than that of V. velutina (not included in previous studies), 60–63 showing a varying degree of sequence homology 63 .…”

Section: Allergic Reactions To Vespa Velutina Venommentioning

confidence: 88%

“…Some 92.6% of these patients presented sIgE to rVes v 5, and the authors concluded that Ves v 5 allergens are the most potent allergens in hornet venom. 59 These results are not surprising, given that antigen 5 was proven to be a dominant allergen in vespid venoms other than that of V. velutina (not included in previous studies), [60][61][62][63] showing a varying degree of sequence homology. 63 The sequence identity described between Vesp c 5 from…”

Section: Vespa Velutinamentioning

confidence: 90%

The Asian wasp Vespa velutina nigrithorax: Entomological and allergological characteristics

Vidal

2021

Clin Experimental Allergy

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The Asian wasp, also known as the Asian predatory wasp or the Asian hornet (Vespa velutina, of the Vespa genus, Vespidae family and Hymenoptera order), was first described in 1836 by the French entomologist Amédée Louis Michel Lepeletier. This species of wasp originated from South-East Asia, in particular the region between Northern India and the Indochinese Peninsula, Taiwan and Indonesia. Due to its extreme success in colonizing new areas, V. velutina is now widespread in other continents and countries where it is considered an invasive species. [1][2][3][4][5][6][7][8][9][10] Although V. velutina Lepeletier 1836 is a well-defined wasp, up to 12 different colour variants geographically distributed throughout Asia have been identified. 11 These colouration patterns and their geographic variations are determined by genes, as have been proven with specific mitochondrial and microsatellite markers; however, the influence of developmental constraints related to crypsis, thermoregulation and aposematism has also been suggested. 11 In general, variations in colouration are a genuine visual aposematic signal that species employ to warn potential predators as a defence against being attacked or eaten. The warning signals displayed by wasps include the colour patterns of the various body segments, using light and black stripes on the abdomen, with yellow, red, black and white the most effective colours. The coloration of V. velutina varies among populations from almost entirely yellow or orange to extensively black. 12,13

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Sensitization of specific IgE-positive Japanese who have experienced Hymenoptera stings to recombinant versions of the Ves v 1 and Ves v 5 allergens in hornet venom

Cited by 4 publications

References 8 publications

Molecular diagnostics and inhibition of cross‐reactive carbohydrate determinants in Hymenoptera venom allergy

Molecular diagnostics and inhibition of cross‐reactive carbohydrate determinants in Hymenoptera venom allergy

Triple IgE-positivity to hornet, wasp and bee venom in the patient with anaphylaxis: Diagnostic and therapeutic approach

The Asian wasp Vespa velutina nigrithorax: Entomological and allergological characteristics

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