Sensitization of vitellogenin gene expression by low doses of octylphenol is mediated by estrogen receptor autoregulation in the Bombina orientalis (Boulenger) male liver

Park, Chan Jin; Gye, Myung Chan

doi:10.1016/j.aquatox.2014.08.013

Cited by 9 publications

(3 citation statements)

References 65 publications

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“…Previous studies showed that the mRNA levels of ER/AR could lead to reproductive and developmental toxicity. , Steroidogenesis might explain the endocrine disruption caused by CBZ . Endogenous estrogens/androgen can regulate the expression of nuclear hormone receptors in fish, , and endocrine disrupters can induce nuclear receptor transcription. , In the present study, CBZ induced significant upregulation of the expression of both er and ar in the gonads and liver, which indicated that CBZ might exert adverse effects associated with ER/AR signaling . In conclusion, CBZ has endocrine-disrupting effects in Chinese rare minnows as demonstrated by changes in gene expression levels.…”

Section: Resultssupporting

confidence: 54%

“…48,49 Steroidogenesis might explain the endocrine disruption caused by CBZ. 50 Endogenous estrogens/androgen can regulate the expression of nuclear hormone receptors in fish, 51,52 and endocrine disrupters can induce nuclear receptor transcription. 53,54 In the present study, CBZ induced significant upregulation of the expression of both er and ar in the gonads and liver, which indicated that CBZ might exert adverse effects associated with ER/AR signaling.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Environmentally Relevant Concentrations of Carbamazepine Caused Endocrine-Disrupting Effects on Nontarget Organisms, Chinese Rare Minnows (Gobiocypris rarus)

Yan

Wang²,

Zha³

et al. 2018

Environ. Sci. Technol.

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In the present study, Chinese rare minnows (Gobiocypris rarus) were exposed to 1, 10, and 100 μg/L of carbamazepine (CBZ) under flow-through conditions for 28 d. A hepatic-specific custom microarray identified 111 and 71 differentially expressed genes in the livers of females and males, respectively, exposed to 100 μg/L of CBZ (ratio ≥ 2, p ≤ 0.05). The levels of five differentially expressed genes associated with the hypothalamic-pituitary-gonadal (HPG) axis were quantified by qPCR, and the results indicated the feasibility of screening endocrine-disrupting chemicals using a custom microarray. The mRNA levels of genes related to the HPG axis differed significantly in different organs of Chinese rare minnows (p < 0.05). Significant differences were observed in the 11-ketotestosterone and plasma vitellogenin levels in all treatments and in the 17β-estradiol (E2) levels in the 100 μg/L CBZ treatment. In contrast, the gonadosomatic index was significantly higher in females and slightly higher in males without significant differences. A pathological analysis determined that 10 and 100 μg/L of CBZ could lead to ova-testis in males and significantly promoted ovum maturation in females. Therefore, our results demonstrate that environmentally relevant concentrations of CBZ have homologous estrogenic activity and induce reproductive toxicity in Chinese rare minnows.

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Section: Resultssupporting

confidence: 54%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Environmentally Relevant Concentrations of Carbamazepine Caused Endocrine-Disrupting Effects on Nontarget Organisms, Chinese Rare Minnows (Gobiocypris rarus)

Yan

Wang²,

Zha³

et al. 2018

Environ. Sci. Technol.

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show abstract

“…The expression of nuclear hormone receptors is regulated by endogenous estrogens/androgen in fish (Bagamasbad and Denver, 2011;Park and Gye, 2014). However, endocrine disrupters, such as nitrophenol, nonylphenol, octylphenol and BPA, also have been shown to induce nuclear receptor transcription (Osachoff et al, 2013;Qiu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Chen

Cao

Yuan

et al. 2016

Environmental Pollution

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a b s t r a c t 2,4-Dichloro-6-nitrophenol (DCNP) is an environmental transformation product of 2,4-dichlorophenol that has been identified as widespread in effluent wastewater, but little is known about its toxicity because this compound is not regulated. Therefore, to investigate the endocrine disruption potency of DCNP in Chinese rare minnows (Gobiocypris rarus), adult and juvenile fish were exposed to various concentrations of DCNP (2, 20, and 200 mg/L) for 28 d. After 28 d exposure, the plasma vitellogenin (VTG) levels were reduced in females while increased in males and juvenile fish considerably, as compared with the control. These results suggested that DCNP affects the HPG-axis in a sex-dependent way. Testosterone (T) levels in the plasma were significantly lower in adult and juvenile fish and were accompanied by an increased estradiol (E2)/T ratio. Histopathological observation revealed hypertrophy of the hepatocytes and nuclear pyknosis in the liver, the inhibition of spermatogenesis in the testes, and the degeneration of oocytes in the ovaries after DCNP exposure. The expression pattern of selected genes indicated that the nuclear receptor, steroidogenesis and gonadotropin regulation pathways were perturbed after DCNP exposure. Above all, our results demonstrated that DCNP clearly had anti-androgenic activity in both adult and juvenile fish and can therefore be considered as an endocrine-disrupting chemical.

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Nickel affects gill and muscle development in oriental fire-bellied toad ( Bombina orientalis ) embryos

et al. 2017

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Sensitization of vitellogenin gene expression by low doses of octylphenol is mediated by estrogen receptor autoregulation in the Bombina orientalis (Boulenger) male liver

Cited by 9 publications

References 65 publications

Environmentally Relevant Concentrations of Carbamazepine Caused Endocrine-Disrupting Effects on Nontarget Organisms, Chinese Rare Minnows (Gobiocypris rarus)

Environmentally Relevant Concentrations of Carbamazepine Caused Endocrine-Disrupting Effects on Nontarget Organisms, Chinese Rare Minnows (Gobiocypris rarus)

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Nickel affects gill and muscle development in oriental fire-bellied toad ( Bombina orientalis ) embryos

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