Sensor and biosensor application of a new redox mediator: Rosmarinic acid modified screen-printed carbon electrode for electrochemical determination of NADH and ethanol

Bilgi, Melike; Sahin, Elif Merve; Ayrancı́, Erol

doi:10.1016/j.jelechem.2018.02.012

Cited by 47 publications

(23 citation statements)

References 49 publications

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“…When the literature data are examined, it is understood that the Epa value of LU observed our study was much lower. The reason for the Epa value of LU being observed at a lower potential in this study may be due to electrochemical pretreatment procedure applied to SPCEs [17]. This result is an advantage for using LU as a mediator in the electrochemical analysis of NADH.…”

Section: Electrochemical Behavior Of Lu At Spce/aunpmentioning

confidence: 68%

“…The difference between the anodic and cathodic peak potentials at scan rate of 10 mV.s -1 was calculated as 36 mV. ∆Ep value is close to 30 mV which is reaction mechanism of a reversible twoelectron [17,20,21,25]. Figure 2 (a) shows CVs of 1 mM LU at SPCE/AuNP electrode with different scanning rates.…”

Section: Electrochemical Behavior Of Lu At Spce/aunpmentioning

confidence: 86%

“…Dilgin et al used pencil graphite electrode (PGE) modified with quercetin for electrocatalytic oxidation of NADH [16]. In recently work, SPCE modified with RA was used for amperometric determination of NADH [17]. Luteolin (LU), 3,4,5,7-tetrahydroxyflavone, is one of the most important flavonoid compounds that has an electroactive catechol group in the B ring [18,19].…”

Section: Introductionmentioning

confidence: 99%

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Mediator effect of luteolin on electrooxidation of NADH

Kamaç

Gylyjova

2021

Sakarya University Journal of Science

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The effects of luteolin (LU) as a new mediator on the electrooxidation of the reduced nicotinamide adenine dinucleotide (NADH) were investigated and developed a new disposable NADH sensor. Firstly, screen printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP), and then, LU was deposited on SPCE modified with AuNP (SPCE/AuNP) using cyclic voltammetry. Electrochemical behavior of LU on SPCE/AuNP was investigated and, the redox proses of LU on SPCE/AuNP was found to be adsorption controlled. The number of cycles was optimized for the electrochemical deposition of LU and determined to be 15 cycles. LU modified SPCE/AuNP (SPCE/AuNP/LU) was found to show the electrocatalytic effect on electrooxidation of NADH and acts as a mediator. Working potential and pH were optimized for electrochemical detection of NADH with the proposed SPCE/AuNP/LU sensor and found to be +0.225 V and 7.25, respectively. The proposed NADH sensor showed a well linear response from 15.90 to 925 μM with a detection limit of 4.57 μM and a sensitivity of 11.19 μAmM −1 . The repeatability of the NADH sensor was tested +0.225 V with 50 µM NADH solution. The relative standard deviation (RSD) was calculated as 3.68% (for n=10). The operational stability studies have shown that the initial amperometric response of sensor to NADH decreased by 62.1% at the 30th day. The analysis of NADH in artificial human serum samples was successfully performed with SPCE/AuNP/LU sensor.

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Section: Electrochemical Behavior Of Lu At Spce/aunpmentioning

confidence: 68%

Section: Electrochemical Behavior Of Lu At Spce/aunpmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

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Mediator effect of luteolin on electrooxidation of NADH

Kamaç

Gylyjova

2021

Sakarya University Journal of Science

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“…Therefore, the linear range, sensitivity and LOD of the developed biosensor were obtained to be 1-29 μM, 0.1025 μA μM À 1 and 0.09 μM respectively. Figure of merits of the developed biosensor were compared with those of the reports and the results were given in Table 1 which reveal comparable and/or better results [37][38][39][40] which suggests it for routine and clinical applications.…”

Section: Figure Of Merits and Analytical Studiesmentioning

confidence: 92%

Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples

Mohammad‐Rezaei

Golmohammadpour

2019

Electroanalysis

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In this study, a sensitive nicotinamide adenine dinucleotide (NADH) biosensor based on Au‐Copper oxide nanocomposite modified carbon ceramic electrode (Au−CuO/CCE) was introduced. The developed NADH biosensor was prepared by controlled electrodeposition of copper and Au nanoparticles on the surface of a renewable CCE and was turned to Au−CuO/CCE by cycling the potential in alkaline media. The prepared electrode was carefully characterized with scanning electron microscopy, X‐ray diffraction, atomic force microscopy and cyclic voltammetry techniques. According to scan rate study, surface coverage (Γ) of the fabricated Au−CuO/CCE was calculated to be 1.54×10−8 mol cm−2 which was 3 time more than CuO/CCE. The fabricated electrode is well stable which could be reliably utilized for the determination of NADH with amperometry technique over the concentration range of 1–29 μM with sensitivity and detection limit (S/N=3) of 0.1025 μA μM−1 and 0.09 μM respectively. The prepared biosensor was used for NADH determination in serum samples with fast response time and satisfactory analytical results.

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“…The great limitation of dehydrogenase-based systems in general, and NAD + -dehydrogenase-based systems, in particular, is that, in contrast to oxidases, which have redox cofactors tighly bound to the enzyme, the cofactors are not bound to the enzyme, and it is necessary to add them to perform each determination [1]. In fact, most of dehydrogenase-based biosensors involve the addition of NAD + cofactor to the supporting electrolyte [5][6][7][8][9][10], but it makes the determination time-consuming and expensive. Therefore, new strategies are required for the construction of reagentless dehydrogenase-based electrochemical biosensors in which both, the enzyme and NAD + , are effectively immobilized onto the electrode in such a way that the cofactor has easy acces to the enzyme.…”

Section: Introductionmentioning

confidence: 99%

Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD⁺/Dehydrogenase‐based Ethanol Biosensor

2019

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This work reports for the first time the development of a reagentless enzymatic amperometric biosensor for ethanol based on the use of a glassy carbon electrode (GCE) modified with multi‐walled carbon nanotubes (MWCNTs) non‐covalently functionalized with polyarginine (Polyarg) as platform for the robust immobilization of alcohol dehydrogenase (ADH) and NAD+. The new strategy allows to obtain an integrated GCE/MWCNTs‐Polyarg/NAD+‐ADH ethanol biosensor with important advantages compared to the existing ethanol biosensors: avoids the external addition of the cofactor for each measurement, ensures a fast and sensitive quantification of ethanol due to the intimate interaction of the components, and allows the detection at considerably lower potentials due to the catalytic activity of the carbon nanostructures. These unique properties have made possible a very efficient ethanol quantification with a sensitivity of (1487±6) μA M−1, detection limit of 0.65 μM, response time of 8 s, and reproducibility of 5.5 % with a very successful application for the quantification of ethanol in different commercial beverages.

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Sensor and biosensor application of a new redox mediator: Rosmarinic acid modified screen-printed carbon electrode for electrochemical determination of NADH and ethanol

Cited by 47 publications

References 49 publications

Mediator effect of luteolin on electrooxidation of NADH

Mediator effect of luteolin on electrooxidation of NADH

Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples

Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD⁺/Dehydrogenase‐based Ethanol Biosensor

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Sensor and biosensor application of a new redox mediator: Rosmarinic acid modified screen-printed carbon electrode for electrochemical determination of NADH and ethanol

Cited by 47 publications

References 49 publications

Mediator effect of luteolin on electrooxidation of NADH

Mediator effect of luteolin on electrooxidation of NADH

Controlled Electrodeposition of Au‐Copper Oxide Nanocomposite on a Renewable Carbon Ceramic Electrode for Sensitive Determination of NADH in Serum Samples

Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD+/Dehydrogenase‐based Ethanol Biosensor

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Product

Resources

About

Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD⁺/Dehydrogenase‐based Ethanol Biosensor