Sensor chip design for increasing surface-plasmon-assisted fluorescence enhancement of the V-trench biosensor

Ashiba, Hiroki; Fujimaki, Makoto; Wang, Xiaomin; Awazu, Kunio; Tamura, Takamasa; Shimizu, Yoshiyuki

doi:10.7567/jjap.55.067001

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…The sensor is equipped with a V-shaped microfluidic channel, which functions as a prism to excite the SPR, and it can perform sensitive biosensing based on SPRF. SPR-induced fluorescence emission on the V-trench was confirmed for fluorescent molecules and quantum dot fluorescent labels in previous studies [ 19 , 20 , 21 ]. Herein, the evaluation of a CNT fluorescent label using a V-trench biosensor designed for CNTs is presented.…”

Section: Introductionsupporting

confidence: 69%

“…In this study, the performance of CNTs as a fluorescent label for target analyte detection using SPRF was evaluated. A V-trench biosensor, which was developed as a miniature and simple SPRF apparatus [ 19 , 20 , 21 ], was used for the evaluation. The sensor is equipped with a V-shaped microfluidic channel, which functions as a prism to excite the SPR, and it can perform sensitive biosensing based on SPRF.…”

Section: Introductionmentioning

confidence: 99%

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Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay

Ashiba

Iizumi

Okazaki

et al. 2017

Sensors

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The photoluminescence properties of carbon nanotubes (CNTs), including the large Stokes shift and the absence of fluorescent photobleaching, can be used as a fluorescent label in biological measurements. In this study, the performance of CNTs as a fluorescent label for surface plasmon resonance (SPR)-assisted fluoroimmunoassay is evaluated. The fluorescence of (8, 3) CNTs with an excitation wavelength of 670 nm and an emission wavelength of 970 nm is observed using a sensor chip equipped with a prism-integrated microfluidic channel to excite the SPR. The minimum detectable concentration of a CNT dispersed in water using a visible camera is 0.25 μg/mL, which is equivalent to 2 × 1010 tubes/mL. The target analyte detection using the CNT fluorescent labels is theoretically investigated by evaluating the detectable number of CNTs in a detection volume. Assuming detection of virus particles which are bound with 100 CNT labels, the minimum number of detectable virus particles is calculated to be 900. The result indicates that CNTs are effective fluorescent labels for SPR-assisted fluoroimmunoassay.

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Section: Introductionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay

Ashiba

Iizumi

Okazaki

et al. 2017

Sensors

Self Cite

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“…Although it is of interest to know details of the molecular structure of the applied SAM, the supplier gave no information concerning this matter. It has to be noted that this type of SAM is assigned especially for biotin–streptavidin assays and was already used . The deposition of the SAM terminated with biotin, followed by streptavidin, and biotinylated horseradish peroxidase onto the sensor surface shows impressively that the biomolecule stack only binds to the gold electrode and not to the PPy:PSS electrode (Figure ).…”

Section: Discussionmentioning

confidence: 99%

“…Although BSA with ≈66 kDa is a large molecule compared with biotin (≈244 Da) and streptavidin (≈52 kDa), control experiments revealed that subsequently applied proteins are not hindered when binding to the biotin of the SAM. Kazuma et al pointed out that BSA does not bind to the SAM itself by means of localized surface plasmon resonance [17b]. Omitting the SAM in the MTP experiments led to absorption values in the photometric measurements of 0.19 without BSA blocking and 0.02 with blocking, respectively.…”

Section: Discussionmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy Using Interdigitated Gold–Polypyrrole Electrode Combination

Kremers

Menzel

Freitag

et al. 2020

Physica Status Solidi (a)

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A novel electrochemical impedance spectroscopy (EIS) sensor design, based on a standard interdigitated electrode arrangement in which the working electrode consists of gold and the combined counter and reference electrodes of polypyrrole doped with polystyrene sulfonate (PPy:PSS), is evaluated for biosensing applications. The performance is successfully proved by immobilization of a thiolated biotin as a self‐assembled monolayer (SAM), followed by streptavidin and a biotinylated horseradish peroxidase. It is shown that specific binding of biomolecules takes place only at the gold electrode. The binding activities are not influenced by the addition of small amounts of the nonionic surfactant Pluronic F‐68. The immobilization process is monitored online with EIS showing an excellent repeatability of the EIS signals in comparison with Au–Au electrode configuration even after electrode regeneration.

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Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels

Ashiba

Sugiyama

Wang

et al. 2017

Biosensors and Bioelectronics

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A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.

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Sensor chip design for increasing surface-plasmon-assisted fluorescence enhancement of the V-trench biosensor

Cited by 4 publications

References 17 publications

Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay

Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay

Electrochemical Impedance Spectroscopy Using Interdigitated Gold–Polypyrrole Electrode Combination

Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels

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