Sensor data as a measure of native freshwater mussel impact on nitrate formation and food digestion in continuous-flow mesocosms

Bril, Jeremy; Durst, Jonathan; Hurley, Brion M.; Just, Craig L.; Newton, Teresa J.

doi:10.1086/675448

Cited by 16 publications

(32 citation statements)

References 39 publications

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“…Complete mixing in each mesocosm was provided by 1,500 L h −1 submersible pumps, and two 1,000-watt solar simulators provided illumination on a 12:12 h light–dark cycle. Additional details regarding the mussel mesocosm system are available elsewhere (Bril et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

“…Sample measurements for org N were estimated by subtracting the sum of NH, NO, and NO from the total N measurements. A more detailed description of the mesocosm sampling and analysis setup is available (Bril et al, 2014). …”

Section: Methodsmentioning

confidence: 99%

“…Seven days of the 107-d experiment were intensively sampled and previously reported (Bril et al, 2014) for food, NH, NO, NO, org N, and temperature; these values were used as model calibration inputs. Linear interpolation between discrete samples was used where 30-min measurements were unavailable (org N, NO, and total N), and ranges for unmeasured model variables (e.g., nitrification rate, denitrification rate) were obtained from the literature (Table 1).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous work showed that native freshwater mussels directly elevate NH and indirectly elevate nitrate (NO) and nitrite (NO) concentrations in lab-based mesocosms (Bril et al, 2014). The increase in NH concentrations by mussels has been associated with ingestion of food (e.g., algae, phytoplankton, bacteria, and fungi), digestion, and subsequent NH excretion (Thorp et al, 1998; Vaughn, Nichols & Spooner, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The model was calibrated using literature values and water chemistry measurements from a separate, 7-d mesocosm sampling period reported in our previous work (Bril et al, 2014). The mussel species Amblema plicata and Lampsilis cardium were selected due to their abundance in the Iowa River (Zohrer, 2006) and throughout the UMR Basin (Newton et al, 2011), where N runoff from industrial agriculture severely impacts the aquatic N cycle.…”

Section: Introductionmentioning

confidence: 99%

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Simulated mussel mortality thresholds as a function of mussel biomass and nutrient loading

Bril

Langenfeld

Just

et al. 2017

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A freshwater “mussel mortality threshold” was explored as a function of porewater ammonium (NH4+) concentration, mussel biomass, and total nitrogen (N) utilizing a numerical model calibrated with data from mesocosms with and without mussels. A mortality threshold of 2 mg-N L−1 porewater NH4+ was selected based on a study that estimated 100% mortality of juvenile Lampsilis mussels exposed to 1.9 mg-N L−1 NH4+ in equilibrium with 0.18 mg-N L−1 NH3. At the highest simulated mussel biomass (560 g m−2) and the lowest simulated influent water “food” concentration (0.1 mg-N L−1), the porewater NH4+ concentration after a 2,160 h timespan without mussels was 0.5 mg-N L−1 compared to 2.25 mg-N L−1 with mussels. Continuing these simulations while varying mussel biomass and N content yielded a mortality threshold contour that was essentially linear which contradicted the non-linear and non-monotonic relationship suggested by Strayer (2014). Our model suggests that mussels spatially focus nutrients from the overlying water to the sediments as evidenced by elevated porewater NH4+ in mesocosms with mussels. However, our previous work and the model utilized here show elevated concentrations of nitrite and nitrate in overlying waters as an indirect consequence of mussel activity. Even when the simulated overlying water food availability was quite low, the mortality threshold was reached at a mussel biomass of about 480 g m−2. At a food concentration of 10 mg-N L−1, the mortality threshold was reached at a biomass of about 250 g m−2. Our model suggests the mortality threshold for juvenile Lampsilis species could be exceeded at low mussel biomass if exposed for even a short time to the highly elevated total N loadings endemic to the agricultural Midwest.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simulated mussel mortality thresholds as a function of mussel biomass and nutrient loading

Bril

Langenfeld

Just

et al. 2017

PeerJ

Self Cite

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Metagenomic analysis of nitrogen‐cycling genes in upper Mississippi river sediment with mussel assemblages

2018

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We investigated the impact of native freshwater mussel assemblages (order Unionoida) on the abundance and composition of nitrogen‐cycling genes in sediment of an upper Mississippi river habitat. We hypothesized that the genomic potential for ammonia and nitrite oxidation would be greater in the sediment with mussel assemblages, presumably due to mussel biodeposition products, namely ammonia and organic carbon. Regardless of the presence of mussels, upper Mississippi river sediment microbial communities had the largest genomic potential for nitrogen fixation followed by urea catabolism, nitrate metabolism, and nitrate assimilation, as evidenced by analysis of nitrogen cycling pathway abundances. However, genes encoding nitrate and nitrite redox reactions, nar GHI and nxr AB , were the most abundant functional genes of the nitrogen cycling gene families. Using linear discriminant analysis ( LDA ), we found nitrification genes were the most important biomarkers for nitrogen cycling genomic potential when mussels were present, and this presented an opposing effect on the abundance of genes encoding nitric oxide reduction. The genes involved in nitrification that increased the most were amoA associated with comammox Nitrospira and nxr homologs associated with Nitrospira . On the other hand, the most distinctive biomarkers of microbial communities without mussels were norB and nrfA , as part of denitrification and dissimilatory nitrate reduction to ammonium pathways, respectively. Ultimately, this research demonstrates the impact of native mollusks on microbial nitrogen cycling in an aquatic agroecosystem.

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Ecosystem services provided by freshwater mussels

2017

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Sensor data as a measure of native freshwater mussel impact on nitrate formation and food digestion in continuous-flow mesocosms

Cited by 16 publications

References 39 publications

Simulated mussel mortality thresholds as a function of mussel biomass and nutrient loading

Simulated mussel mortality thresholds as a function of mussel biomass and nutrient loading

Metagenomic analysis of nitrogen‐cycling genes in upper Mississippi river sediment with mussel assemblages

Ecosystem services provided by freshwater mussels

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