Bioanalytical chemistry and biosensor research have achieved great success since the first biosensor development in the 1960s. Biosensor has its unique advantages to achieve fast, real‐time analysis at a low cost with small integrated and portable device compared with conventional analytical techniques. It represents a promising tool for clinic diagnosis, environmental monitoring, and many other areas. While only small part of biosensors have been applied for practical and commercial applications, technical challenges and economic barrier are needed to be overcome for many biosensors toward practical applications. This article covers principles, basic types, construction of biosensor, current situation, and research trends of biosensors.