The integration of Digital Twins (DTs) is becoming increasingly important in various industries. This entails the need for a comprehensive and practical IT infrastructure framework. This paper presents a modifiable medium-level architecture that serves as a link between established reference architectures such as RAMI 4.0 and the pragmatic implementation of Digital Twins. The functionalities of an IT infrastructure are considered, and functional hardware and software components for fulfilling these are described. The proposed architecture is suitable for various deployment scenarios, including local, cloud and hybrid cloud–edge configurations. In order to improve the applicability of the medium-level architecture, a step-by-step procedure is also proposed, which helps to transfer the overarching requirements for a Digital Twin into a suitable IT infrastructure. Finally, the results are demonstrated by an exemplary application to a two-stage industrial gearbox.