Sensorial, structural and functional response of rats subjected to hind limb immobilization

Júnior, Francisco Fleury Uchôa Santos; Pires, Alana de Freitas; Ribeiro, Natália Matos; Mendonça, Vanessa; Alves, Juliana Osório; Soares, Paula Matias; Ceccatto, Vânia Marilande; Assreuy, Ana Maria Sampaio

doi:10.1016/j.lfs.2015.07.020

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…Another limitation is that pain-related behaviors and pathological mechanisms associated with limb immobilization might differ across immobilization models [6][7][8][9][10][11][12][13][14]. As shown in Fig.…”

Section: Study Limitationsmentioning

confidence: 99%

“…In rodents, long-term cast immobilization of a limb following fracture and tissue injury leads to nociceptive hypersensitivity similar to that seen in patients with complex regional pain syndrome [ 2 ], muscular atrophy [ 3 ], osteoporosis [ 4 ], and joint contracture [ 5 ]. To date, several models of limb immobilization-induced pain have been reported using different induction methods (cast [ 6 – 8 ], waterproof tape [ 9 ], wire [ 10 ], and suspension of tail/hindlimb [ 11 , 12 ]), extremities (forelimb [ 8 , 13 ] and hindlimb [ 6 , 7 ]), and immobilization periods (2–8 weeks [ 6 , 7 , 13 , 14 ]).…”

Section: Introductionmentioning

confidence: 99%

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Responses of cutaneous C-fiber afferents and spinal microglia after hindlimb cast immobilization in rats

et al. 2021

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Previous studies have shown that persistent limb immobilization using a cast increases nociceptive behavior to somatic stimuli in rats. However, the peripheral neural mechanisms of nociception remain unclear. Using single-fiber electrophysiological recordings in vitro, we examined the general characteristics of cutaneous C-fiber afferents in the saphenous nerve and their responsiveness to mechanical and heat stimuli in a rat model of immobilization-induced pain by subjecting the rats to hindlimb cast immobilization for 4 weeks. The mechanical response of C-fibers appeared to increase in the model; however, statistical analysis revealed that neither the response threshold nor the response magnitude was altered. The general characteristics and heat responses of the C-fibers were not altered. The number of microglia and cell diameters significantly increased in the superficial dorsal horn of the lumbar spinal cord. Thus, activated microglia-mediated spinal mechanisms are associated with the induction of nociceptive hypersensitivity in rats after persistent cast immobilization.

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Section: Study Limitationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Responses of cutaneous C-fiber afferents and spinal microglia after hindlimb cast immobilization in rats

et al. 2021

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“…Sensory changes, such as hyperalgesia, can occur after immobilization. In addition, there are changes in the neural system associated with motor disuse by the activation of primary nociceptive neurons [5][6][7] . The longer the inactivity or immobilization, the greater the effects on the body's systems.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of nociception induced by whole-body vibration remobilization in Wistar rats

Wutzke¹,

Peretti²,

Ribeiro³

et al. 2020

Brazilian Journal of Pain

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BACKGROUND AND OBJECTIVES:The vibrating platform can attenuate the deleterious effects of immobilization related to muscle atrophy. However, there is still a gap regarding the effect of this modality on hyperalgesia related to immobilization. The objective of this study was to analyze the effect of remobilization with whole-body vibration on the nociception of Wistar rats. METHODS: Sixteen rats were randomly distributed into two groups: the FRG group -immobilization and free remobilization and the VPRG -immobilization and remobilization with the vibrating platform. For remobilization with the vibrating platform, the frequency of 60Hz for 10 minutes, five days a week for two weeks was used. The nociception was evaluated on the right paw by a digital analgesiometer, before and at the end of the immobilization, and after two weeks of remobilization. RESULTS: There were differences between evaluations but not between groups, indicating that immobilization reduced the nociceptive threshold and free remobilization, and the remobilization associated with vibration improved the nociceptive threshold compared to the post-immobilization moment. However, they were not able to return to the initial parameters. CONCLUSION: Joint immobilization reduced the nociceptive threshold; however, two weeks of whole-body vibration remobilization were not able to revert the threshold in the immobilized groups.

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“…Both situations may limit the patients' full abilities such as their locomotion and some activities of daily life 1,2 . Small periods of movement restriction, even short-term ones, can lead to several damages to the immobilized region 3 , including disorders in various body segments and organic systems, such as circulatory impairments 1 , ligament alterations 4 , increase of the connective issue 5 , edema 6 , articular rigidity 4 , muscle hypertrophy and atrophy 7 and reduction in bone mineral density 3,8 .…”

Section: Introductionmentioning

confidence: 99%

“…These damages in the mechanical structure of the diaphragm and the consequent reduction of diaphragmatic movement and thoracic excursion can cause an increase in the mechanical resistance and a decrease of the pulmonary ventilation, leading to atelectasies and pneumonias 9 . Several studies approach the impact of immobilization on the locomotor system [1][2][3]6,7 ; however, little is known about the respiratory system regarding the effects of devices that constrain the torso movement. In this context, the present study examined the impact that the restriction of abdominal movement from an experimental model containing multiple motor limitations can promote on the structure and function of the respiratory system and whether they are reversible with recruitment maneuver.…”

Section: Introductionmentioning

confidence: 99%

Análise da função pulmonar e estrutura micromecânica após 14 dias de restrição de movimento em ratas

Júnior¹,

Souza

Serra

et al. 2017

Fisioter. Pesqui.

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| Immobilization is a condition that affects several segments and organic systems, including the respiratory system, leading to structural and functional alterations. The purpose of this study was to analyze pulmonary function and micromechanical structure after 14 days of movement restriction in rats. Fourteen female Wistar rats with body mass between 210±50 g were used, divided into two groups, composed of (n=7) each group: Control (C) and Immobilized (I).The immobilization procedure involved the abdomen (and last ribs), pelvis, hip and knee extension and the ankle in plantar flexion in the two week period. After the immobilization period, an analysis of the pulmonary function was performed using a mechanical ventilator for small animals, flexVent, and alveolar recruitment maneuvers. Subsequently, lung strips were removed from each animal for pulmonary micromechanics analysis.

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Sensorial, structural and functional response of rats subjected to hind limb immobilization

Cited by 8 publications

References 28 publications

Responses of cutaneous C-fiber afferents and spinal microglia after hindlimb cast immobilization in rats

Responses of cutaneous C-fiber afferents and spinal microglia after hindlimb cast immobilization in rats

Evaluation of nociception induced by whole-body vibration remobilization in Wistar rats

Análise da função pulmonar e estrutura micromecânica após 14 dias de restrição de movimento em ratas

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