Sensory stimulus evokes inhibition rather than excitation in cerebellar Purkinje cells in vivo in mice

Chu, Chun-Ping; Bing, Yan-Hua; Qiu, De-Lai

doi:10.1016/j.neulet.2010.10.018

Cited by 37 publications

(62 citation statements)

References 28 publications

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“…Anesthetical and surgical procedures have been described previously [21]. The experimental procedures were approved by the Animal Care and Use Committee of Yanbian University and they were in accordance with the animal welfare guidelines of the U.S. National Institutes of Health.…”

Section: Anesthesia and Surgical Proceduresmentioning

confidence: 99%

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Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo

Pan

Bing

et al. 2018

Pharmacology

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Etomidate is an imidazole, nonbarbiturate hypnotic agent that is increasingly used in procedural sedation. However, the effects of etomidate on the spontaneous activity of cerebellar Purkinje cells (PCs) in living mouse have not been fully understood. In this study, we investigated the effects of etomidate on the spontaneous simple spike (SS) activity of PCs in urethane-anesthetized mice by cell-attached recording and pharmacological methods. Cerebellar surface application of etomidate (50 μmol\L) reduced the SS firing rate in a concentration-dependent manner (IC50: 43.4 µmol\L). Application of either a γ-aminobutyric acid type A (GABA_A) receptor antagonist, SR95531 (20 μmol\L) or a glycine receptor antagonist strychnine (10 μmol\L) significantly attenuated but not abolished the etomidate-induced decrease in PC SS firing rate. However, co-application of SR95531 (20 μmol\L) and strychnine (10 μmol\L) abolished the etomidate-induced decrease in PC SS firing rate. Moreover, intraperitoneal injection of etomidate (3 mg/kg body weight) also induced a significant depression in PC SS firing rate, which was blocked by the co-application of SR95531 and strychnine on the cerebellar surface. These results indicate that both GABA_A and glycine receptors are involved in the etomidate-induced decrease in PC SS firing rate in vivo in mice.

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Section: Anesthesia and Surgical Proceduresmentioning

confidence: 99%

“…Recording electrodes were filled with ACSF and had resistances of 3-5 MΩ. PCattached recordings were performed at depths of 250-350 μm under the pia mater membrane and identified by regular spontaneous simple spikes (SSs) accompanied by irregularly complex spikes (CSs) [21].…”

Section: Cell-attached Recordingsmentioning

confidence: 99%

Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo

Pan

Bing

et al. 2018

Pharmacology

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“…citatory component, N2 ( fig. 4 a), which represents the sensory stimulation-evoked PC excitation [12] . Co-application of gabazine and propofol (300 μmol/l) induced less effect on the amplitude of N2 (p > 0.05; fig.…”

Section: Effects Of Propofol On Facial Stimulation-evoked Field Potenmentioning

confidence: 99%

“…With the exception of GCs, cerebellar cortical neurons are primarily GABAergic inhibitory neurons [10] . PCs exhibit 2 distinct types of spike firing patterns, simple spikes and complex spikes, which enable their identification by in vivo recording [11,12] . The MLIs receive excitatory input from PFs and inhibitory input from other interneurons, and they exert GABAergic inhibition on PCs [10,13,14] .…”

Section: Introductionmentioning

confidence: 99%

“…The MLIs receive excitatory input from PFs and inhibitory input from other interneurons, and they exert GABAergic inhibition on PCs [10,13,14] . We recently found that sensory stimulation evokes a GABA A receptor-mediated field potential response in the ML, and induces rapid excitation in MLIs and inhibition occurring at later latencies in PCs in the Crus II in the mouse cerebellar cortex [12,15,16] . In addition, the MLI network acts as a low-pass filter in sensory information processing, and plays a critical role in modulating sensory-related outputs from PCs in the mouse cerebellar cortex [17] .…”

Section: Introductionmentioning

confidence: 99%

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Effects of Propofol on the Dynamic Properties of Sensory Information Processing in the Mouse Cerebellar Cortical Molecular Layer in vivo

Jin

Shi²,

Liu³

et al. 2015

Pharmacology

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Propofol is a global central nervous system depressant that affects information processing in the central nervous system. However, the effects of propofol on sensory information processing in the cerebellar cortical molecular layer are unknown. In this study, we examined the effects of propofol on the dynamics of sensory stimulation-evoked responses in the cerebellar molecular layer in urethane-anesthetized mice, using electrophysiological and pharmacological methods. Our results showed that cerebellar surface perfusion of propofol (10-1,000 μmol/l) significantly decreased amplitude and area under the curve (AUC) of the sensory stimulation-evoked inhibitory component (P1) but increased the rise time and decay time of P1. In contrast, administration of propofol significantly enhanced the sensory stimulation-evoked excitatory component (N1), which exhibited increases in amplitude and AUC, as well as increases in rise time and decay time. By blocking the GABA_A receptor activity, propofol failed to increase the amplitude and the AUC of the excitatory postsynaptic component (N2) of PCs. Our present results suggest that propofol modulates the dynamic properties of the sensory information processing in the cerebellar molecular layer through the modulation of GABA_A receptors activity in the adult mouse.

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Computational Structure of the Cerebellar Molecular Layer

Bower

2021

Handbook of the Cerebellum and Cerebellar Disorders

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Theories regarding the computational structure of the cerebellar molecular layer have been more strongly influenced by biological data than is the case for many and perhaps even most other vertebrate brain regions. Yet, or perhaps for this reason, the computational significance of this circuitry remains a very active subject of discussion and debate, with even basic assumptions regarding the physiological organization of this circuit now being questioned. Specifically, while most current models of molecular layer circuitry assume that parallel fiber excitatory synaptic input directly drives Purkinje cells to fire, there is growing experimental evidence that this might not be the case. Recent modelbased efforts to better understand the functional role of the parallel fiber system suggest that molecular layer inhibition may play a larger and more complex

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Sensory stimulus evokes inhibition rather than excitation in cerebellar Purkinje cells in vivo in mice

Cited by 37 publications

References 28 publications

Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo

Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo

Effects of Propofol on the Dynamic Properties of Sensory Information Processing in the Mouse Cerebellar Cortical Molecular Layer in vivo

Computational Structure of the Cerebellar Molecular Layer

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