Senyawa Fenolik dari Daun Tanaman Kalanchoe prolifera (Crassulaceae)

Yun, Yenny Febriani; Aisyah, Lilis Siti; Saputra, Tri Reksa; Hakim, Arif Rahman; Purbaya, Sari; Herlina, Tati; Julaeha, Euis; Zainuddin, Achmad; Supratman, Unang

doi:10.15408/jkv.v0i0.5037

Cited by 3 publications

(3 citation statements)

References 2 publications

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“…Kaempferol and quercetin with pelargonidin, cyanidin, peonidin, delphinidin, petunidin, and malvidin-derived compounds were analyzed in K. blossfeldiana flowers [49]. Epigallocatechin gallate and gallic acid have already been detected in the leaves of K. blossfeldiana [50,51]. In turn, referring to the chemical composition of K. daigremontiana water fraction described in the previously published work [22], this extract contained mostly flavonol glycosides-quercetin, kaempherol, patuletin, myricetin and isorhamnetin derivatives-and isoflavones.…”

Section: Discussionmentioning

confidence: 89%

Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities

Stefanowicz–Hajduk

Hering

Kowalczyk

et al. 2023

Plants

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Kalanchoe species are succulents occurring in tropical regions. They have many biological and pharmacological properties. In this study, the cytotoxic and antimicrobial activities of water and dichloromethane Kalanchoe fractions obtained from ethanol extracts of three Kalanchoe species—K. daigremontiana, K. pinnata, and K. blossfeldiana were estimated. The cytotoxic effect was assessed on human cancer cell lines—ovarian SKOV-3, cervical HeLa, breast MCF-7, and melanoma A375—by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antimicrobial activity was estimated on selected Gram-positive and Gram-negative bacteria strains and on Candida albicans. The phytochemical analysis of selected Kalanchoe extracts was conducted by LC-QTOF-MS. The obtained results showed that the water fraction of K. blossfeldiana was active both on the tested cancer cells (IC50 values were 28.28 ± 2.76 and 32.51 ± 0.69 µg/mL on HeLa and SKOV-3, respectively) and bacteria strains (MIC values were 16 and 32 µg/mL on S. epidermidis and S. aureus, respectively). The water fraction of K. pinnata also had a significant effect on S. epidermidis and S. aureus, with MIC values of 32 and 64 µg/mL, respectively. The water fraction of K. blossfeldiana triggered a decrease in mitochondrial membrane potential (MMP) and induced cell cycle arrest in the G2/M phase in the SKOV-3 and HeLa cells. This fraction did not significantly increase cellular oxidative stress level. The DPPH and ABTS assays revealed that the water fraction of K. blossfeldiana had a strong antioxidant effect (IC50 was 9.44 ± 0.06 and 3.17 ± 0.1 µg/mL, respectively). The phytochemical analysis of the extracts of K. blossfeldiana and K. pinnata revealed the presence of at least 218 main components. The most frequently occurring were flavonol glycosides (31 metabolites), phenylpropanoids (13 metabolites), gallic acid derivatives (13 compounds), benzoic acid derived compounds (14 metabolites), and acyclic alcohol glycosides (16 compounds). In addition, proanthocyanidins were detected mainly in K. blossfeldiana. The study indicates that the water fraction of K. blossfeldiana has significant biological potential and can be further investigated towards anticancer and antimicrobial application.

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Section: Discussionmentioning

confidence: 89%

Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities

Stefanowicz–Hajduk

Hering

Kowalczyk

et al. 2023

Plants

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“…Sepuluh sinyal proton ini merupakan kerangka alkil (Salempa et al, 2016). Pergeseran tersebut kemudian dibandingkan dengan senyawa β-sitosterol (Yun et al, 2017) dan memiliki banyak kesamaan. Maka dapat disimpulkan bahwa senyawa tersebut merupakan senyawa β-sitosterol.…”

Section: Hasil Dan Pembahasan Ekstraksi Dan Isolasi Senyawaunclassified

Senyawa Steroid dari Cocor Bebek (Kalanchoe tomentosa) sebagai Antibakteri Pseudomonas aeruginosa

et al. 2021

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Plak adalah pembentukan komunitas bakteri yang terorganisir pada permukaan gigi yang berupa lapisan tipis tidak berwarna. Pseudomonas aeruginosa adalah salah satu bakteri yang sangat berperan pada pembentukan plak. Metabolit sekunder yang terkandung dalam tanaman Kalanchoe tomentosa dapat digunakan sebagai antibakteri. Tahapan penelitian diawali dengan maserasi menggunakan pelarut n-heksan dan diklorometana. Ekstrak diklorometana dipisahkan menggunakan Kromatografi Cair Vakum (KCV) dengan n-heksana dan etil asetat sebagai pelarut bergradien selanjutnya direkristalisasi dengan n-heksan, hasil rekristalisasi didapatkan isolat 1. Isolat 1 dikarakterisasi dengan spektroskopi infra-red (IR), ultraviolet (UV) dan nuclear magnetic resonance hydrogen (1H NMR) serta dibandingkan dengan literatur. Isolat 1 diketahui merupakan stigmast-5-en-3ꞵ-ol atau ꞵ-sitosterol. Pada ekstrak diklorometana dan senyawa ꞵ-sitosterol kemudian ditentukan aktivitas antibakteri secara mikrodilusi didapatkan hasil bahwa ekstrak diklorometana dan senyawa ꞵ-sitosterol bukan hanya menunjukan sifat bakteriostatik tetapi juga bakterisid kuat terhadap bakteri Pseudomonas aeruginosadengan nilai Konsentrasi Hambat Minimum (KHM) <100 µg/mL.Steroid Compounds from Cocor Bebek (Kalanchoe tomentosa) as Antibacterial Pseudomonas aeruginosa. Plaque is a thin, colorless layer that adheres tightly to the surface of the teeth and contains a collection of bacteria. One of the bacteria that can form dental plaque is Porphyromonas aeruginosa. Kalanchoe tomentosa contain secondary metabolite which can be used as antibacterial. Stages of the study begin with maceration using n-hexane and dichloromethane. Dichloromethane extract was separated using Vacuum Liquid Chromatography (KCV) using n-hexane and ethyl acetate solvents and then recrystallized with n-hexane, the recrystallization results were obtained by isolate 1. Isolate 1 was marked by IR spectroscopy, and 1HNMR and compared with literature. Isolate 1 is known as stigmast-5-en-3ꞵ-ol or ꞵ-sitosterol. In dichloromethane extracts and ꞵ-sitosterol is determined by microdilution by microdilution. The results showed that dichloromethane extract and ꞵ-sitosterol compound not only showed strong bacteriostatic but also bacterisid activity against P. aeruginosa with Minimum Inhibiroty Concentration (MIC) <100 μg / mL.

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“…Salah satu jenis Garcinia yang sudah banyak diteliti yaitu Garcinia mangostana atau dikenal sebagai buah manggis. 19 Garcinia spp. telah terbukti menjadi sumber yang kaya senyawa yang memiliki efek terapeutik.…”

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Skrining Fitokimia dan Profil KLT Ekstrak dan Fraksi Daun Manggu Leuweung (Garcinia celebica L.)

Peratiwi,

Tahara,

Mustikawati

et al. 2023

ijbp

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Tanaman manggu leuweung (Garcinia celebica L.) merupakan tanaman yang termasuk genus Garcinia danfamili Clusiaceae. Genus Garcinia spp. telah banyak diteliti karena memiliki potensi untuk dikembangkansebagai alternatif pengobatan namun belum banyak penelitian yang dilakukan pada ekstrak danfraksi daun G. celebica. Dilaporkan ekstrak dari daun G. celebica memiliki aktivitas sebagai antioksidan,antimikroba, antikanker dan antiplasmodial. Tujuan dari penulisan ini untuk mengetahui kandungansenyawa metabolit sekunder dan profil KLT yang pada ekstrak etanol, fraksi n-heksan dan fraksi etilasetat daun G. celebica. Penelitian dilakukan melalui tahap ekstraksi dengan metode maserasi, fraksinasisecara Ekstraksi Cair-Cair (ECC), pemeriksaan ekstrak dan fraksi dengan Kromatografi Lapis Tipis(KLT) dan skrining fitokimia. Berdasarkan hasil skrining fitokimia ekstrak daun G. celebica mengandungsenyawa flavonoid, saponin, polifenol, monoterpen/seskuiterpen. Fraksi n-Heksana daun G. celebicamengandung senyawa flavonoid, polifenol dan monoterpen/seskuiterpen sedangkan fraksi etil asetatdaun G. celebica L. mengandung senyawa flavonoid, saponin, polifenol, monoterpen/seskuiterpen.

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Senyawa Fenolik dari Daun Tanaman Kalanchoe prolifera (Crassulaceae)

Cited by 3 publications

References 2 publications

Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities

Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities

Senyawa Steroid dari Cocor Bebek (Kalanchoe tomentosa) sebagai Antibakteri Pseudomonas aeruginosa

Skrining Fitokimia dan Profil KLT Ekstrak dan Fraksi Daun Manggu Leuweung (Garcinia celebica L.)

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