Separation and analysis of Monascus yellow pigment produced on durian seed substrate

Srianta, Ignatius; Nugerahani, Ira; Ristiarini, Susana

doi:10.26656/fr.2017.4(4).020

Cited by 16 publications

(26 citation statements)

References 14 publications

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“…Actinomycetes, a subgroup of filamentous Gram-positive bacteria within the class Actinomycetales , have provided many important clinical drugs [ 1 – 2 ], agrochemicals [ 3 ], food additives [ 4 – 5 ], and biochemical reagents [ 6 – 9 ], and continue to be a core source of bioactive molecules [ 10 ]. While most of the actinomycetes-derived compounds have been reported from the genus Streptomyces [ 11 ], non- Streptomyces actinomycetes, commonly referred to as rare actinomycetes [ 12 ], are attracting considerable attention as less tapped taxa for drug discovery.…”

Section: Introductionmentioning

confidence: 99%

Unsaturated fatty acids and a prenylated tryptophan derivative from a rare actinomycete of the genus Couchioplanes

Saito¹,

Indo²,

Oku³

et al. 2021

Beilstein J. Org. Chem.

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A genome mining survey combined with metabolome analysis of publicly available strains identified Couchioplanes sp. RD010705, a strain belonging to an underexplored genus of rare actinomycetes, as a producer of new metabolites. HPLC-DAD-guided fractionation of its fermentation extracts resulted in the isolation of five new methyl-branched unsaturated fatty acids, (2E,4E)-2,4-dimethyl-2,4-octadienoic acid (1), (2E,4E)-2,4,7-trimethyl-2,4-octadienoic acid (2), (R)-(−)-phialomustin B (3), (2E,4E)-7-hydroxy-2,4-dimethyl-2,4-octadienoic acid (4), (2E,4E)-7-hydroxy-2,4,7-trimethyl-2,4-octadienoic acid (5), and one prenylated tryptophan derivative, 6-(3,3-dimethylallyl)-N-acetyl-ʟ-tryptophan (6). The enantiomer ratio of 4 was determined to be approximately S/R = 56:44 by a recursive application of Trost’s chiral anisotropy analysis and chiral HPLC analysis of its methyl ester. Compounds 1–5 were weakly inhibitory against Kocuria rhizophila at MIC 100 μg/mL and none were cytotoxic against P388 at the same concentration.

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Section: Introductionmentioning

confidence: 99%

Unsaturated fatty acids and a prenylated tryptophan derivative from a rare actinomycete of the genus Couchioplanes

Saito¹,

Indo²,

Oku³

et al. 2021

Beilstein J. Org. Chem.

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“…Microbial secondary metabolites have been used as therapeutic drugs [1], veterinary medicines [2], agrochemicals [3], food preservatives/colorings [4,5], medium supplements for selective microbial/cell culture [6][7][8], or biochemical reagents for pharmacological/chemical biology studies [9] and continue to be indispensable to support and improve human welfare and social life. In recent years, in accordance with the advancement of genome and in silico analytical technologies, the searching process for new microbial secondary metabolites became faster and more efficient [10].…”

Section: Introductionmentioning

confidence: 99%

A cyclopeptide and three oligomycin-class polyketides produced by an underexplored actinomycete of the genus Pseudosporangium

Saito¹,

Atsumi²,

Zhou³

et al. 2020

Beilstein J. Org. Chem.

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Aside from the well-studied conventional actinomycetes such as Streptomyces, the less investigated genera of actinomycetes also represent a promising source of natural products. Genome mining indicated that members of the underexplored genus Pseudosporangium, from which no secondary metabolites have been reported to date, may harbor the biosynthetic machinery for the formation of novel natural products. The strain RD062863, that is available at a public culture collection, was obtained and subjected to metabolite analysis, which resulted in the discovery of a novel cyclopeptide, pseudosporamide (1), along with three new oligomycin-class polyketides, pseudosporamicins A–C (2–4). The unusual structure of compound 1, featured by a biaryl-bond bridging across a tripeptide scaffold, N-acetyl-ʟ-Tyr-ʟ-Pro-ʟ-Trp, was determined by a combination of spectroscopic analyses, chemical derivatization, ECD calculation, and DFT-based theoretical chemical shift calculation, revealing the presence of an (S a)-axial chirality around the biaryl bond. Compounds 2–4 lacked hydroxylation on the side chain of the spiroacetal rings, which showed clear contrast to other oligomycin congeners and related polyketides with ring-truncation or expansion. The new macrolides 2–4 displayed potent antimicrobial activity against the Gram-positive bacterium Kocuria rhizohpila and the plant pathogenic fungus Glomerella cingulata. All compounds showed moderate cytotoxicity against P388 murine leukemia cells with IC50 values in the micromolar to submicromolar ranges. These results exemplified the validity of phylogeny-focused strain selection combined with biosynthetic gene-directed genome mining for the efficient discovery of new natural products.

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“…It is reported that Monascus yellow pigment have encouraging potential for application in food and pharmaceutical industries based on their bioactivities. Anti-atherosclerosis effect, antiobesity activity [26,27], antitumor activity [28,29], memory and learning ability [30] have also been reported for monascin and ankaflavin.…”

Section: Introductionmentioning

confidence: 86%

“…The HPTLC method is used as a valuable method for evaluating the emerging types of natural products [31]. The Monascus pigments were identified in HPTLC plate as visible bands of red, orange and yellow colour when scanned at different wavelengths [32]. The most recent HPTLC method enables useful fingerprint analysis to determine the identity and composition of natural phytomolecules.…”

Section: Introductionmentioning

confidence: 99%

Biopigments Produced by Monascus purpureus Through Solid State Fermentation: HPTLC Finger Printing Analysis

Panda¹,

Ahmad²

2023

SVOAMB

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The current study aims to conduct HPTLC finger printing analysis of various extracts of Monascus purpureus biopigments (water extract, acetone extract, chloroform extract, acetonitrile extract, petroleum ether extract, methanol extract, ethanol extract, hexane extract). Natural and synthetic pigments both play an important roles as biocolourants in the food and pharmaceutical industries. The most recent HPTLC technique helps useful fingerprint analysis to determine the identity and composition of natural phytomolecules. Solid state fermentation was carried out with the help of a production medium contains long grain, unripe basmati rice. Water, acetone, chloroform, petroleum ether, acetonitrile, ethanol, methanol, and hexane were used to extract fermented rice (5g), which was then analysed at 485 nm. For scanning at different wavelengths of 370 nm (yellow pigment), 420 nm (red pigment), 470 nm (orange pigment), 500 nm, and 530 nm (red pigment), various mobile phases have been developed. When chromatographed with mobile phase 5 (ethyl acetate: isopropanol: water-8.0:1.0:1.0 v/v), an acetone extract of Monascus biopigment showed good resolution.

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Separation and analysis of Monascus yellow pigment produced on durian seed substrate

Cited by 16 publications

References 14 publications

Unsaturated fatty acids and a prenylated tryptophan derivative from a rare actinomycete of the genus Couchioplanes

Unsaturated fatty acids and a prenylated tryptophan derivative from a rare actinomycete of the genus Couchioplanes

A cyclopeptide and three oligomycin-class polyketides produced by an underexplored actinomycete of the genus Pseudosporangium

Biopigments Produced by Monascus purpureus Through Solid State Fermentation: HPTLC Finger Printing Analysis

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