Separation and characterization of gold nanoparticle mixtures by flow-field-flow fractionation

Calzolai, Luigi; Gilliland, Douglas; García, César Pascual; Rossi, François

doi:10.1016/j.chroma.2011.01.017

Cited by 102 publications

(66 citation statements)

References 26 publications

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“…The comparatively large value reported for NM-300 by Nanoparticle Tracking Analysis System can be related to the limit of detection of the method, which is not reliable for nanoparticles typically below 30-50 nm depending on the NPs optical properties [27][28][29]. Results obtained for NM-300 size distribution by DLS were comparable with those previously observed [30] and indicate a polydispersity of the sample, and therefore an unreliable value of the size measured by DLS [31]. These results were complemented by TEM size distribution analysis and CPS, which indicate both an average diameter of 15-17 nm (see supplementary information).…”

Section: Ag Nps Characterizationsupporting

confidence: 77%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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Section: Ag Nps Characterizationsupporting

confidence: 77%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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“…All these methods have limitations; SEM because the amount of particles measured is limited; DLS is more sensitive to bigger particles, while the sensitivity of disc centrifuging is very much density dependant. However, the agreement of the three methods allows us to discard aggregation, or the existence of smaller species masked by larger ones, a typical phenomena occurring with DLS measurements 14 . Therefore, by using three complementary techniques we carefully characterized the AuNPs according to size, which is critical for the interpretation of results obtained using cellular interactions.…”

Section: Resultsmentioning

confidence: 99%

Microscopic Analysis of the Interaction of Gold Nanoparticles with Cells of the Innate Immune System

García

Sumbayev

Gilliland

et al. 2013

Sci Rep

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Gold nanoparticles (AuNPs) can interfere with some of the biochemical processes of macrophage cells but the mechanisms of action of these potentially medically-relevant effects are still unclear. Here we study the fate of AuNPs interacting with cells derived from the innate immune system. To visualize AuNPs with nanometer resolution without losing sight of the whole morphology of cells we developed a convenient approach that uses electron and ion microscopy techniques. The inspection using an ion beam to selectively cut where the AuNPs were found, allows for determining their intracellular localizations. We studied the cellular uptake of AuNPs, with or without exposure of the cells to Latrunculin-A, a phagocytosis inhibitor. Results indicate a size dependence of the internalization mechanisms for THP-1 cells. The internalization of larger AuNPs was blocked in the presence of Latrunculin-A, although they could attach to the membrane. Smaller AuNPs though were not blocked by actin depending processes.

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“…The small gold nanospheres had a very regular shape (Fig. 1), narrow size distribution (2r = 15.6 ± 1.6 nm), and narrow absorption peak (518 nm), while the gold nanoflowers were of irregular shape, larger size, and highly polydisperse (40-120 nm), with an absorption well extended into the NIR region [48,49]. Big regularly shaped gold nanospheres with a hydrodynamic diameter of 86 nm were employed to identify effects that are caused by large particle size.…”

Section: Characterization Of Gold Nanospheres and Gold Nanoflowersmentioning

confidence: 99%

Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia

Kodiha

Hutter

Boridy

et al. 2014

Cell. Mol. Life Sci.

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nanospheres, damaged the nucleus at normal growth temperature, several of these defects were further exacerbated by mild hyperthermia. Taken together, the toxicity of gold nanoparticles correlated with changes in nuclear organization and function. These results emphasize that the cell nucleus is a prominent target for gold nanoparticles of different morphologies. Moreover, we demonstrated that RNA synthesis in nucleoli provides quantitative information on nuclear damage and cancer cell survival.

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Separation and characterization of gold nanoparticle mixtures by flow-field-flow fractionation

Cited by 102 publications

References 26 publications

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Microscopic Analysis of the Interaction of Gold Nanoparticles with Cells of the Innate Immune System

Gold nanoparticles induce nuclear damage in breast cancer cells, which is further amplified by hyperthermia

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