Separation and colorimetric detection of Escherichia coli by phage tail fiber protein combined with nano-magnetic beads

Separation mixed semen of two individuals using magnetic beads coupled ABH blood group antibody

“…Immunomagnetic beads, which are uniform and suitable‐size polystyrene microspheres, are effective in isolating cells through magnetization and their connection with antibodies [20, 21]. Their main characteristics include: (i) fast separation speed, high efficiency, and good repeatability; (ii) simple operation without the need for expensive equipment; (iii) minimal impact on the biological characteristics and functions of the isolated cells; (iv) specific separation achieved by coupling specific antibodies and probes [22]. Magnetic bead‐based separation has been widely used in clinical diagnosis, isolation and detection of various tumor cells, bacteria, and other microorganisms due to its high efficiency, rapidity, simplicity, non‐toxicity, low cost, high separation purity, and retention of cell activity [23–25].…”

Optical Sensors for Bacterial Detection

“…The capabilities of a colorimetric method for detecting bacteria (E. coli) in water that is based on the magnetic separation of proteins of the tail fibers of a T7 phage were shown in [30]. The capture efficiency for E. coli ranged from 88.70 to 95.65%, and 10 2 CFU/mL of E. coli could be detected by the naked eye.…”

Rapid and sensitive detection of glucocorticoids using engineered magnetosomes functionalized protein A conjugated broad-spectrum monoclonal antibody

“…By harnessing their unique composition, function, and magnetic properties, immunomagnetic beads offer unprecedented benefits for separation and analysis of targets ( Hong et al, 2023 ; Tao et al, 2023 ). Therefore, the future of immunomagnetic bead technology holds tremendous promise for further advancement in multiple domains.…”