Separation and Cultivation of Hematopoietic Stem Cells from Umbilical Cord Blood by Permeation through Membranes with Nano-Segments

Higuchi, Akon; Chen, Li Ying; Shiao, Jui Shiang; Ling, Qing; Ko, Yi An; Chang, Yung; Yu, Chang; Bing, Jun Tang; Hsu, Shih Tien

doi:10.2174/157341311798220637

Cited by 4 publications

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“…Higuchi et al polymerized GMA on PS dishes or polyurethane foaming membranes where the epoxy group was generated on the surface of the dishes and membranes. [206][207][208] Subsequently, the amine group or peptide could be conjugated on the surface with the reaction of the deoxy group and ammonia or amine group of the peptide (CS1, EILDVPST) in aqueous solution for human hematopoietic stem cell (HSC) culture. 209 Furthermore, methacrylated peptides can be prepared by reacting the amino groups of peptides and GMA.…”

Section: Chemical Reaction For the Immobilization Of Peptidesmentioning

confidence: 99%

Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

et al. 2023

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Section: Chemical Reaction For the Immobilization Of Peptidesmentioning

confidence: 99%

Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

et al. 2023

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The combined influence of substrate elasticity and surface-grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells

et al. 2013

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Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenvironment on HSPC expansion, we investigated the ex vivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. Polyvinylalcohol-co-itaconic acid (PVA-IA)-coated dishes with different stiffnesses ranging from a 3.7 kPa to 30.4 kPa storage modulus were used. Fibronectin or an oligopeptide (CS1, EILDVPST) was grafted onto the PVA-IA substrates. High ex vivo fold expansion of HSPCs was observed in the PVA-IA dishes grafted with fibronectin or CS1, which displayed an intermediate stiffness ranging from 12.2 kPa to 30.4 kPa. The fold expansion was more than 1.4 times higher than that cultured in tissue culture polystyrene dishes (TCPS, 12 GPa). Furthermore, HSPCs cultured in fibronectin or CS1-grafted PVA-IA-coated dishes with a stiffness of 12.2-30.4 kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the ex vivo expansion of HSPCs.

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Stem Cell: Hematopoietic Stem Cell Culture, Materials for

Higuchi¹,

Yang²,

Li³

et al. 2016

Encyclopedia of Biomedical Polymers and Polymeric Biomaterials

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Separation and Cultivation of Hematopoietic Stem Cells from Umbilical Cord Blood by Permeation through Membranes with Nano-Segments

Cited by 4 publications

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Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

Cell-binding peptides on the material surface guide stem cell fate of adhesion, proliferation and differentiation

The combined influence of substrate elasticity and surface-grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells

Stem Cell: Hematopoietic Stem Cell Culture, Materials for

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