Separation and Detection of Cyanine-Labeled Amino Acids by Micellar Electrokinetic Chromatography Combined with Fluorescence Detection Using Diode-Based Solid-State Lasers

Kaneta, Takashi; Komatsubara, T. K.; Shiba, Hiroki; Imasaka, Totaro

doi:10.2116/analsci.14.1017

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…There were a few labeling reagents with fundamental molecular structures of cyanine dyes and thionine dyes that could be excited by deep red lights. Cyanine dyes were successfully employed in the determination of amino acids and their enantiomers . As light sources, a diode laser emitting at 635 nm and a diode laser‐pumped Nd:YAG laser emitting at 532 nm (second harmonic wave) were employed in the detection of amino acids labeled with cyanine dyes …”

Section: Overview Of Ce‐lifmentioning

confidence: 99%

Laser‐Induced Fluorometry for Capillary Electrophoresis

Kaneta

2018

The Chemical Record

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Laser-induced fluorometry (LIF) has achieved the detection of single molecules, which ranks it among the most sensitive of detection techniques, whereas capillary electrophoresis (CE) is known as a powerful separation method with resolution that is beyond the reach of many other types of chromatography. Therefore, a coupling of LIF with CE has established an unrivaled analytical technique in terms of sensitivity and resolution. CE-LIF has demonstrated excellent performance in bioanalytical chemistry for the high-resolution separation and highly sensitive detection of DNAs, proteins, and small bioactive molecules. This review describes the CE-LIF methods developed by the author's group that include indirect and direct detection using diode lasers, post-column derivatization, and Hadamard transformation, as well as applications to the binding assays of specific DNA immunoassays of proteins and to the determination of anticancer drugs.

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Section: Overview Of Ce‐lifmentioning

confidence: 99%

Laser‐Induced Fluorometry for Capillary Electrophoresis

Kaneta

2018

The Chemical Record

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“…In earlier papers, Cy5 had been used for investigating the fluorescence labeling of proteins (BSA) [8][9][10][11] and diagnosing the 21-hydroxylase deficiency [12]. Amino acids [13,14], amantadine [15], mAb [16], monoamine neurotransmitters [17], and phosphorus-containing amino acid herbicides [18] have also been determined by Cy5 with diode LIF detection. To date, despite several methods have been established, few of them are involved in the quantification of biologically significant analytes derivatized by Cy5.…”

Section: Introductionmentioning

confidence: 99%

Quantification of polyamines in human erythrocytes using a new near‐infrared cyanine 1‐(ϵ‐succinimidyl‐hexanoate)‐ 1′‐methyl‐3,3,3′,3′‐tetramethyl‐ indocarbocyanine‐5,5′‐disulfonate potassium with CE‐LIF detection

Zhang

et al. 2007

Electrophoresis

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A novel near-infrared (NIR) cyanine 1-(epsilon-succinimidyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium (MeCy5-OSu) has been developed in our laboratory. Simultaneous determination of MeCy5-OSu-derivatized polyamines spermine (Spm), spermidine (Spd), cadaverine (Cad), and putrescine (Put) based on the separation by CE combined with diode LIF detection has been accomplished. The highest derivatization efficiency was achieved in 0.2 mol/L borate buffer (pH 8.8) for 20 min at 25 degrees C. Polyamine derivatives were separated within 14 min in the phosphate running buffer (pH 3) containing 50 mmol/L phosphoric acid, 40 mmol/L SDS, and 35% methanol v/v. Linearity of response was obtained in the range of 10-200 nmol/L. The detection limits (S/N = 3) for Spm, Spd, Cad, and Put were 0.8, 1, 3, and 2 nmol/L, respectively. The proposed method has been successfully applied to the analysis of polyamines in erythrocytes of two healthy persons and one cancer patient. Average recoveries for erythrocyte samples were 93.6-106% and coefficients of variation ranged from 1.8 to 5.4%. The analysis of polyamines in erythrocytes can be used for studying the relationship between their changes and the carcinogenesis process involved in erythrocytes.

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“…An indocyanine fluorescence dye, sulfoindocyanine succinimidyl ester (Cy5), has recently been developed and used for investigating the fluorescence labeling of proteins (BSA) [20][21][22][23] and diagnosing the 21-hydroxylase deficiency [24]. Amino acids [25,26], amantadine [27], mAb [28], and monoamine neurotrasmitters such as noradrenaline and dopamine [29] have also been determined with high sensitivity by diode laser LIF combined with CZE [25,28] and MEKC [26,27,29].…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive determination of phosphorus-containing amino acid herbicides in soil samples by capillary zone electrophoresis with diode laser-induced fluorescence detection

Orejuela

Silva

2005

Electrophoresis

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A straightforward and sensitive method has been developed for the analysis of phosphorus-containing amino acid herbicides (glufosinate and aminomethylphosphonic acid, the major metabolite of glyphosate) in soil samples. For this purpose, the analytical features of two indocyanine fluorescent dyes, sulfoindocyanine succinimidyl ester (Cy5) and 1-ethyl-1-[5-(N-succinimidyl-oxycarbonyl)pentyl]-3,3,3,3-tetramethyl-indodicarbocyanine chloride, as labeling reagents for the determination of these herbicides by CZE with diode LIF detection were investigated. Practical aspects related to the labeling chemistry and CZE separation showed that the two probes behave similarly, Cy5 being the best choice for the determination of these herbicides on account of its higher sensitivity. The optimum procedure includes a derivatization step of the pesticides at 25 degrees C for 30 min and direct injection to CZE analysis, which is conducted within about 14 min using ACN in the running buffer. The lowest detectable analyte concentration ranged from 0.025 to 0.18 microg/L with a precision of 3.6-5.4%. These results indicate that indocyanine fluorescence dyes are useful as rapid and sensitive labels for the determination of these herbicides when compared with typical fluorescein dyes such as FITC and 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein, because they provide faster labeling reactions even at room temperature and the excess of reagent practically does not interfere the determination. Finally, the Cy5 method was successfully applied to soil samples without a preliminary clean-up procedure, and the herbicides were measured without any interference from coexisting substances. The recoveries of these compounds in these samples at fortification levels of 100-500 ng/g were 90-93%.

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Separation and Detection of Cyanine-Labeled Amino Acids by Micellar Electrokinetic Chromatography Combined with Fluorescence Detection Using Diode-Based Solid-State Lasers

Abstract: Keywords Micellar electrokinetic chromatography, laser-induced fluorescence detection, diode laser, diode laserpumped YAG laser, amino acid † To whom correspondence should be addressed.

Cited by 11 publications

References 14 publications

Laser‐Induced Fluorometry for Capillary Electrophoresis

Laser‐Induced Fluorometry for Capillary Electrophoresis

Quantification of polyamines in human erythrocytes using a new near‐infrared cyanine 1‐(ϵ‐succinimidyl‐hexanoate)‐ 1′‐methyl‐3,3,3′,3′‐tetramethyl‐ indocarbocyanine‐5,5′‐disulfonate potassium with CE‐LIF detection

Rapid and sensitive determination of phosphorus-containing amino acid herbicides in soil samples by capillary zone electrophoresis with diode laser-induced fluorescence detection

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