Separation and determination of active components in Radix Salviae miltiorrhizae and its medicinal preparations by nonaqueous capillary electrophoresis

Abstract

A nonaqueous capillary electrophoresis (NACE) method was developed for simultaneous assay of three bioactive components (1: cryptotanshinone; 2: tanshinone IIA, and 3: tanshinone I) in Radix Salviae miltiorrhizae and in its herbal preparations for the first time. After optimization of separation conditions, a buffer of 250 mmol L(-1) ammonium acetate containing 30% acetonitrile and 1.0% acetic acid (V:V) in methanol was selected for separating the three analytes, but baseline separation of tanshinon I and tanshinone IIA was not obtained. Therefore second-order derivative electropherograms were applied for resolving overlapping peaks. Regression equations revealed good linear relationships (correlation coefficients 0.9943-0.9991) between peak heights in second-order derivative electropherograms and concentrations of the three analytes. The relative standard deviations (RSD) of the migration times and the peak height of the three constituents were in the range of 0.81 -0.88% and 0.34-1.13% (intra-day), 1.57-1.86% and 3.05-5.52% (inter-day), respectively. The recoveries of three constituents ranged from 90.2 to 108.5%. The results indicated that baseline separation of the analytes was sometimes hard to obtain and second-order derivative electropherograms were applicable for the resolving and analysis of overlapping peaks.

Tanshinone IIA decreases the migratory ability of AGS cells by decreasing the protein expression of matrix metalloproteinases, nuclear factor κB-p65 and cyclooxygenase-2

“…Tanshinone IIA (Tan-IIA; C 19 H 18 O 3 ) is extracted from Danshen (Salviae miltiorrhizae radix) (1,2). Tan-IIA exerts antitumor activity in a variety of human cancer cells, such as lung (3), breast (4,5), hepatic (6), pancreatic (7) and colon (8).…”

Tanshinone IIA inhibits the growth of pancreatic cancer BxPC-3 cells by decreasing protein expression of TCTP, MCL-1 and Bcl-xL

“…Traditional herbs are widely accepted as a valid method of treatment of various forms of human cancer and a considerable effort to develop alternative medicines is currently underway (3). Tanshinone IIA (Tan-IIA; C 19 H 18 O 3 ) is one of the active constituents of Danshen (4,5). Tan-IIA is toxic to numerous human cancer cells, including Colo205 colon cancer (6), MDA-MB-231 breast cancer (7), A-549 non-small cell lung cancer (8), H-146 small cell lung cancer (9) and Hep-J5 hepatocellular carcinoma cells (10).…”

Tanshinone IIA potentiates the efficacy of 5-FU in Colo205 colon cancer cells in vivo through downregulation of P-gp and LC3-II

“…Danshen (Salviae miltiorrhizae Radix) is widely prescribed in traditional Chinese medicine for cardiovascular diseases (6,7). Tanshinone IIA (Tan-IIA; C 19 H 18 O 3 ) is extracted from Danshen (8,9), and possesses anti-inflammatory (10,11) and antioxidant properties (12,13). Our previous studies have shown that Tan-IIA inhibits growth and induces apoptosis in Colo205 human colon cancer cells (14) and MDA-MB-231 breast cancer cells in vitro (15).…”