Separation and determination of peptide metabolite of <i>Bacillus licheniformis</i>
 in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

Wang, Wei; Bai, Ruiguang; Cai, Xiaoyu; Lin, Ping; Ma, Lihong

doi:10.1002/jssc.201700656

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…A high‐speed capillary electrophoresis (HSCE) technique, proposed by Fang et al in recent years, could achieve very high separation efficiency and needed less separation time (<5 min) and sample volume (picoliters). Our previous work also introduced manual sample introduction devices for HSCE to separate amino acids . HSCE is also an ideal means for the separation of miRNAs.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of microRNAs by high‐speed capillary sieving electrophoresis

Wang

Cai

Lin

et al. 2018

J of Separation Science

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In this work, high-speed capillary sieving electrophoresis with laser-induced fluorescence detection was applied to simultaneously determine three microRNAs. A developed manual sample introduction device for the high-speed capillary electrophoresis system was applied to perform sample injection. Strategies, including field-amplified sample injection and electrokinetic injection, were studied to improve the detection sensitivity. Under the optimal conditions, the limit of detection for DNA-159 could be lowered to 5.10 × 10 mol/L. In order to achieve enough separation resolution, two DNA probes were designed to have extra sequences that acted as the drag tails. Under the optimized conditions, the three DNA probes and the complexes of microRNA-156, microRNA-159, and microRNA-166 could be completely separated within 3.2 min in background electrolyte (pH 8.7) containing 2.0% m/m polyvinyl pyrrolidone and 0.4% m/m hydroxyethyl cellulose. The limits of detection for the three microRNAs were 0.051, 0.11, and 0.25 nmol/L, respectively. Then the method was applied to analyze the microRNAs spiked in the samples extracted from banana leaves. The recoveries ranged from 114.3 to 121.1% (n = 3). The results showed that the method developed in this work was an effective means for microRNA assay.

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Section: Introductionmentioning

confidence: 99%

Separation and determination of microRNAs by high‐speed capillary sieving electrophoresis

Wang

Cai

Lin

et al. 2018

J of Separation Science

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“…The metabolism process of bacteria highly depends on the supply of the culture mediums . Bacteria can avail specific substrate to produce certain AAs . To analyze the AA metabolites produced by C. glutamicum fed with different culture mediums is significant to realize the metabolism process of this bacterium.…”

Section: Introductionmentioning

confidence: 99%

“…Coupled with sensitive LIF detection, HSCE had become a powerful means for the analysis of low‐concentration AAs (1–100 nM). In our previous works , HSCE–LIF was successfully applied for the separation and detection of AAs and peptides in laver and MFCs.…”

Section: Introductionmentioning

confidence: 99%

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

et al. 2019

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Corynebacterium glutamicum (C. glutamicum) is a well‐known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high‐speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum, which is fed with different culture mediums. Corynebacterium glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. In the CE system, a microliter‐scale sample reservoir was designed and applied to perform tiny volume sample injection. With the assistance of microwave, the derivatization time for amino acids with FITC was greatly shortened to 6 min. Under the optimized condition, the eight candidate amino acids of metabolites could be rapidly separated within 2 min. The whole analysis process for real samples, from sampling to determination, could be shortened to less than 10 min. The results showed that C. glutamicum could produce additional l‐lysine and l‐valine as the metabolites when fed with glucose and l‐methionine, respectively. The method proved that culture mediums used to feed C. glutamicum had great effect on the bacterium's metabolites.

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“…Micellar electrokinetic capillary chromatography (MEKC) is one of the modes of CE, which is frequently used for analysis of uncharged or partially charged chemical compounds. Moreover, the separation efficiency of MEKC can be improved by adding different additives such as surfactants, cyclodextrins (CDs) and organic modifiers . Cyclodextrin‐modified micellar electrokinetic capillary chromatography (CD‐MEKC) is a MEKC method which used CDs as additives, that demonstrated an efficient way for separation and determination of chemical compounds from crude drug.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the separation efficiency of MEKC can be improved by adding different additives such as surfactants, cyclodextrins (CDs) and organic modifiers. [14][15][16][17] Cyclodextrin-modified micellar electrokinetic capillary chromatography (CD-MEKC) is a MEKC method which used CDs as additives, that demonstrated an efficient way for separation and determination of chemical compounds from crude drug. For example, Eder et al developed a CD-MEKC method, consisting of γ-CD and sodium dodecyl sulphate (SDS), to separate doxorubicin and doxorubicinol.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin‐modified micellar electrokinetic capillary chromatography

Guo

Wang

Zhang

et al. 2019

Phytochemical Analysis

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Introduction Hirsutine and hirsuteine are the main pharmacological activity ingredients of Uncaria rhynchophylla (UR), playing an important role in treating mental and cardiovascular diseases, such as Alzheimerʼs disease, hypertension, Parkinson's disease, potential anti‐cancer activities and so on. Objective To develop a cyclodextrin‐modified micellar electrokinetic capillary chromatography (CD‐MEKC) method for the simultaneous separation and determination of hirsutine and hirsuteine from UR and its formulations. Methodology The optimal method was developed by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of hirsutine and hirsuteine in UR and its formulations. Results The optimal background electrolyte (BGE) consisted of 40 mM sodium dihydrogen phosphate (pH 7.0), 150 mM 2,6‐dimethyl‐β‐cyclodextrin (DM‐β‐CD), 3 mM mono‐(6‐ethylenediamine‐6‐deoxy)‐β‐cyclodextrin (ED‐β‐CD), and 30 mM sodium cholate (SC). Under these conditions, hirsutine and hirsuteine were successfully separated within 13 min at the separation voltage of 15 kV, temperature of 25°C and the detection wavelength of 224 nm. For the analytes, linear calibration curves were performed within the range 5.0–160.0 μg/mL. The limit of detection (LOD, S/N = 3) and the limit of quantitation (LOQ, S/N = 10) were 0.41, 1.42 μg/mL for hirsutine and 0.60, 2.17 μg/mL for hirsuteine, respectively. The recoveries of three samples were from 97.9% to 102.3%. Conclusion The method was successfully applied to the determination of hirsutine and hirsuteine in UR and its formulations. Meanwhile, it provides an effective reference of the quality control of UR and its formulations.

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Separation and determination of peptide metabolite of Bacillus licheniformis in a microbial fuel cell by high-speed capillary micellar electrokinetic chromatography

Cited by 12 publications

References 31 publications

Separation and determination of microRNAs by high‐speed capillary sieving electrophoresis

Separation and determination of microRNAs by high‐speed capillary sieving electrophoresis

Study of the effect of culture mediums on the amino acid metabolites for Corynebacterium glutamicum using high‐speed micellar electrokinetic chromatography

Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin‐modified micellar electrokinetic capillary chromatography

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