Separation and Determination of Trace Dinitropyrenes by Means of Off-Line Reduction-HPLC-Chemihiminescence Detection. Application to Assessing Atmospheric Environment

Abstract

A flow injection analysis (FIA) system using a bis(2,4,6-trichlorophenyl)oxalate (TCPO)-hydrogen peroxide (H2O2) chemiluminescence (CL) system was developed for the determination of small amounts of polycyclic aromatic hydrocarbons (PAHs). The system allowed us to determine PAH over a wide range of concentrations with the detection limits (S/ N=5) of 0.05 pg (perylene), 65 pg (anthracene), and 75 pg (pyrene). On the basis of the results, an off-line reduction-HPLC-CL detection system was developed for separation and determination of dinitropyrenes (DNPs) as the specific PAH. DNPs were reduced to diaminopyrenes (DAPs), which were then analyzed by the HPLC equipped with the CL detector. A metal-free system was developed by use of plastic-frame column and all Teflon-made line. These new devices have improved the sensitivity and the reproducibility of determination; DNPs were determined over a wide range of concentration with the detection limit (S/N=5) of 0.025 pg (1,8-, 1,6-DNP) and 0.05 pg (1,3-DNP). The present off-line reduction-HPLC system was applied to the determination of individual DNP at trace levels in the atmosphere.

Determination of 3,6-dinitrobenzo[e]pyrene in Surface Soil and Airborne Particles, and Its Possible Sources, Diesel Particles and Incinerator Dusts

“…In addition, the particle-size-distribution of 3,6-DNBeP was compared to those of 1,3-, 1,6-, and 1,8-DNP isomers which are known as representative airborne contaminants. [18][19][20] Diesel particles and incinerator dusts, which were anticipated as sources of 3,6-DNBeP, were collected. 3,6-DNBeP and DNP isomers in diesel particles and incinerator dusts were analyzed to reveal their sources, and their amounts were compared.…”

Analytical Applications of Liquid Phase Chemiluminescence Reactions — A Review

“…Polycyclic aromatic hydrocarbons (PAHs), e.g., benzo[a]pyrene and chrysene, and substituted derivatives, e.g., diaminopyrenes and nitropyrenes have been detected at fg-pg levels using peroxyoxalate CL (134)(135)(136)(137)(138)(139). Methods for their determination involved reversed-phase separation with an acetonitrile mobile phase prior to CL detection.…”

“…Nitro-PAHs have been determined by LC-CL using a variety of reduction techniques to convert the nitro compounds into the corresponding amines. Both on-line (137.138) and off-line (134)(135)(136)139), pre-column reduction techniques have been reported. One particular method involved on-line reduction in a water-methanol mobile phase by a catalyst column packed with material originating from a grained automobile three-way catalyst (137).…”